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EGb761 Blocks MPP+-Induced Lipid Peroxidation in Mouse Corpus Striatum

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Abstract

EGb761 has been suggested to be an antioxidant and free radical scavenger. Excess generation of free radicals, leading to lipid peroxidation (LP), has been proposed to play a role in the damage to striatal neurons induced by 1-methyl-4-phenylpyridinium (MPP+). We investigated the effects of EGb761 pretreatment on MPP+ neurotoxicity. C-57 black mice were pretreated with EGb761 for 17 days at different doses (0.63, 1.25, 2.5, 5 or 10 mg/kg) followed by administration of MPP+, (0.18, 0.36 or 0.72 mg/kg). LP was analyzed in corpus striatum at 30 min, 1 h, 2 h and 24 h after MPP+ administration. Striatal dopamine content was analyzed by HPLC at the highest EGb761 dose at 2 h and 24 h after MPP+ administration. MPP+-induced LP was blocked (100%) by EGb761 (10 mg/kg). Pretreatment with EGb761 partially prevented (32%) the dopamine-depleting effect of MPP+ at 24 h. These results suggest that supplements of EGb761 may be effective at preventing MPP+-induced oxidative stress.

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Authors and Affiliations

  1. Laboratory of Neurotoxicology, Instituto Nacional de Neurología y Neurocirugía, “Manuel Velasco Suárez” SS, Av. Insurgentes Sur No. 3877, C.P. 14269, México, D.F., México

    Patricia Rojas, Belén Garduño & Norma Serrano-Garcia

  2. Instituto de Investigaciones Biomédicas, Department of Physiology, Universidad Nacional Autónoma de México Apartado, Postal 70228 C.P. 04510, México, D.F., México

    Carolina Rojas

  3. Laboratory of Histomorphology, Instituto Nacional de Pediatría, Av. Insurgentes Sur No. 3700-C, Col. Insurgentes Cuicuilco, C.P. 04530, México, D.F., México

    Rosa María Vigueras & Julio Rojas-Castañeda

  4. Department of Neurochemistry, Instituto Nacional de Neurología y Neurocirugía, “Manuel Velasco Suárez” SS, Av. Insurgentes Sur No. 3877, C.P. 14269, México, D.F., México

    Camilo Ríos

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  1. Patricia Rojas
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Rojas, P., Garduño, B., Rojas, C. et al. EGb761 Blocks MPP+-Induced Lipid Peroxidation in Mouse Corpus Striatum. Neurochem Res 26, 1245–1251 (2001). https://doi.org/10.1023/A:1013971524150

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