Abstract
In this work it is shown that the bienzyme lipase/lipoxygenase system can function in reversed micelles of bis(2-ethyl)hexyl sulfosuccinate (AOT) in octane. As a lipase substrate, a fish fat preparation (fat of sea mammals) with a high content of polyunsaturated fatty acids was used. It was demonstrated that the bienzyme reaction proceeded in a stationary mode and had a rate-limiting step catalyzed by lipase. Under optimal conditions, the efficacy of functioning of the bienzyme system was by an order of magnitude higher than that in water. The lipase/lipoxygenase bienzyme system can be used as a new method of spectrophotometrical determination of lipase activity.
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Pavlenko, I.M., Kuptsova, O.S., Klyachko, N.L. et al. The Lipase/Lipoxygenase Bienzyme System in AOT Reversed Micelles in Octane. Russian Journal of Bioorganic Chemistry 28, 44–49 (2002). https://doi.org/10.1023/A:1013954219627
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DOI: https://doi.org/10.1023/A:1013954219627