Abstract
The plant pathogen, Xanthomonas campestris NRRL B-1459 was chromosomally tagged with gfp, and the transformant, which was subjected to Southern hybridization showed the presence of gfp in the chromosome. The virulence-related gene of the transformant was not affected by the insertion of gfp. After inoculation into cabbage plants, the infection process was visually studied in planta. Using a fluorescence microscope, the migration and distribution of gfp-labelled bacteria was visualized in real time. As the gfp-labelled cells were easily visualized from the beginning of infection, we observed a time delay of 2 days between distribution of the Xanthomonas cells in cabbage plant and the appearance of visible necrosis.
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So, JS., Lim, H.T., Oh, ET. et al. Visualizing the infection process of Xanthomonas campestris in cabbage using green fluorescent protein. World Journal of Microbiology and Biotechnology 18, 17–21 (2002). https://doi.org/10.1023/A:1013925428500
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DOI: https://doi.org/10.1023/A:1013925428500