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Regulation of ammonium-induced proline accumulation in detached rice leaves

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Abstract

Accumulation of proline in response to NH4Cl was studied indetached leaves of rice (Oryza sativa cv. Taichung Native1). Increasing concentrations of NH4Cl from 50 to 200mMprogressively increased proline content and this was correlated with theincrease in ammonium content. Proline accumulation induced by NH4Clwas related to proteolysis, an increase in ornithine-δ-aminotransferaseactivity, a decrease in proline dehydrogenase activity, and a decrease inproline utilisation and could not be explained by NH4Cl-inducedmodification in Δ1-pyrroline-5-carboxylate reductase activity.The content of glutamic acid was decreased by NH4Cl, whereas theincrease in arginine and ornithine contents was found to be associated with theincrease in proline content in NH4Cl-treated detached rice leaves.

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References

  • Aziz A., Martin-Tanguy J. and Larher F. 1998. Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride. Physiol. Plant. 104: 195–202.

    Google Scholar 

  • Bassi R. and Sharma S.S. 1993. Changes in proline content accompanying the uptake of zinc and copper by Lemna minor. Ann. Bot. 72: 151–154.

    Google Scholar 

  • Bates L.S., Waldren S.P. and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39: 205–207.

    Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Google Scholar 

  • Brown D.H. and Fowden L. 1966. Metabolism of _-acetylornithine in two Leguminous species. Phytochemistry. 5: 887–892.

    Google Scholar 

  • Charest C. and Phan C.T. 1990. Cold acclimation of wheat: Properties of enzymes involved in proline metabolism. Physiol. Plant. 80: 159–168.

    Google Scholar 

  • Chen L.-M. and Kao C.H. 1998. Relationship between ammonium accumulation and senescence of detached rice leaves caused by excess copper. Plant Soil. 200: 169–173.

    Google Scholar 

  • Chen S.L. and Kao C.H. 1995. Cd induced changes in proline content and peroxidase activity in roots of rice seedlings. Plant Growth Regul. 17: 67–71.

    Google Scholar 

  • Chen S.J., Hung K.T. and Kao C.H. 1997. Ammonium accumulation is associated with senescence of rice leaves. Plant Growth Regul. 21: 195–201.

    Google Scholar 

  • Chiang H.-H. and Dandekar A.M. 1995. Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and in response to desiccation. Plant Cell Environ. 18: 1280–1290.

    Google Scholar 

  • Chien H.-F. and Kao C.H. 2000. Accumulation of ammonium in rice leaves in response to excess cadmium. Plant Sci. 156: 111–115.

    Google Scholar 

  • Chou I.T., Chen C.T. and Kao C.H. 1990. Regulation of proline accumulation in detached rice leaves. Plant. Sci. 70: 43–48.

    Google Scholar 

  • Coleman R.G. and Hegarty M.P. 1957. Metabolism of D, L-ornithine-214C in normal and potassium-defficient barley. Nature. 179: 376–377.

    Google Scholar 

  • Delauney A.J. and Verma D.P.S. 1993. Proline biosynthesis and osmoregulation in plants. Plant. J. 4: 215–223.

    Google Scholar 

  • Guerrier G. 1995. Effect of salt-stress on proline metabolism in calli of Lycopersicon esculentum, Lycopersicon pennellii, and their interspecific hybrid. Can. J. Bot. 73: 1939–1946.

    Google Scholar 

  • Hare P.D., Cress W.A. and van Staden J. 1999. Proline synthesis and degradation: a model system for elucidating stress-related signal transduction. J. Exp. Bot. 333: 413–434.

    Google Scholar 

  • Ireland A.J. and Lea P.J. 1999. The enzymes of glutamine, glutamate, asparagine, and aspartate metabolism. In: Singh B.K. (ed.), Plant Amino Acids. Mercel Dekker Inc, New York,pp. 49–109.

    Google Scholar 

  • Johnson C.M., Stout P.R., Broyer T.C. and Carlton A.B. 1957. Comparative choline requirements of different plant species. Plant. Soil. 8: 337–353.

    Google Scholar 

  • Lin J.-N. and Kao C.H. 1998. Water stress, ammonium, and leaf senescence in detached rice leaves. Plant Growth Regul. 26: 165–169.

    Google Scholar 

  • Lin J.-N., Wang J.-W. and Kao C.H. 1999. Effect of abscisic acid and water stress on the senescence of detached rice leaves. Biol. Plant. 42: 313–316.

    Google Scholar 

  • Lingnowski E.M. and Splittstoessor W.E. 1971. The change in arginine levels and metabolism of urea and ornithine in Cucubita moschatta seedlings. Physiol. Plant. 25: 225–229.

    Google Scholar 

  • Lutts S., Majerus V. and Kinet J.M. 1999. NaCl effects on proline metabolism in rice (Oryza sativa L.) seedlings. Physiol. Plant. 105: 450–458.

    Google Scholar 

  • Madan S., Nainawatee H.S., Jain R.K. and Chowdhury J.B. 1995. Proline and proline metabolising enzymes in in-vitro selected NaCl-tolerant Brassica juncea L. under salt stress. Ann. Bot. 76: 51–57.

    Google Scholar 

  • Mattioni C., Lacerenza N.G., Troccoli A., De Leonardis A.M. and Di Fonzo N. 1997. Water and salt stress-induced alterations in proline metabolism of Triticum durum seedlings. Physiol. Plant. 101: 787–792.

    Google Scholar 

  • Postitus C. and Jacobi G. 1976. Dark starvation and plant metabolism. VI. Biosynthesis of glutamic acid dehydrogenase in detached leaves of Cucurbita maxima. Z. Pflanzenphysiol. 78: 133–140.

    Google Scholar 

  • Rabe E. 1990. Stress physiology: The functional significance of the accumulation of nitrogen-containing compounds. J. Hort. Sci. 65: 231–243.

    Google Scholar 

  • Rena A.B. and Splittstoesser W.E. 1975. Proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotyledons. Phytochemistry. 14: 657–661.

    Google Scholar 

  • Roosens N.H.C.J., Thu T.T., Iskandar H.M. and Jacobs M. 1998. Isolation of the ornithine-_-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana. Plant. Physiol. 117: 263–271.

    Google Scholar 

  • Savoure A., Hua X.-J., Bertauche N., van Montagu M. and Verbruggen N. 1997. Abscisic acid-independent and abscisic aciddependent regulation of proline biosynthesis following cold and osmotic stresses in Arabidopsis thaliana. Mol. Gen. Genet. 254: 104–109.

    Google Scholar 

  • Thomas H. 1978. Enzymes of nitrogen mobilization in detached leaves of Lolium temulentum during senescence. Planta. 142: 161–169.

    Google Scholar 

  • Vogel R.H. and Kopac M.J. 1960. Some properties of ornithine-_-transaminase from Neurospore. Biochem. Biphys. Acta. 37: 539–540.

    Google Scholar 

  • Wang C.Y., Cheng S.H. and Kao C.H. 1982. Senescence of leaves VII. Proline accumulation in senescing excised leaves. Plant. Physiol. 69: 1348–1349.

    Google Scholar 

  • Wu J.-T., Chang S.J. and Chou T.-L. 1995. Intracellular proline accumulation in algae exposed to copper and cadmium. Bot. Bull. Acad. Sin. 36: 69–93.

    Google Scholar 

  • Yang C.-W. and Kao C.H. 1999. Ammonium in relation to proline accumulation in detached rice leaves. Plant Growth Regul. 30: 139–144.

    Google Scholar 

  • Yang C.-W., Lin C.C. and Kao C.H. 1999. Endogenous ornithine and arginine contents and dark-induced proline accumulation in detached rice leaves. J. Plant. Physiol. 155: 665–668.

    Google Scholar 

  • Yang C.-W., Wang J.-W. and Kao C.H. 2000. The relation between accumulation of abscisic acid and proline in detached rice leaves. Biol. Plant. 43: 301–304.

    Google Scholar 

  • Yoshiba Y., Kiyosue T., Nakashima K., Yamaguchi-Shinozaki K. and Shinozaki K. 1997. Regulation of levels of proline as an osmolyte in plants under water stress. Plant. Cell Physiol. 38: 1095–1102.

    Google Scholar 

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  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Chuan Chi Lin & Ching Huei Kao

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  1. Chuan Chi Lin
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  2. Ching Huei Kao
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Lin, C.C., Kao, C.H. Regulation of ammonium-induced proline accumulation in detached rice leaves. Plant Growth Regulation 35, 69–74 (2001). https://doi.org/10.1023/A:1013832630830

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