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Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation

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Abstract

Recombinant human interleukin-3 (rhIL-3) production by a Streptomyces lividans strain is subject to severe proteolytic degradation. Human interleukin-3 (rhIL-3) concentration in the culture supernatant typically reaches 100–120 mg l−1 about 20 h after inoculation, but the titer drops rapidly as the result of proteolysis. Reducing the culture temperature at this point has a dramatic effect on product yield, increasing the final concentration in the supernatant at least two-fold.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1

    Soon-Il Yun, Ahmad R.M. Yahya, William A. Anderson, Jeno M. Scharer & Murray Moo-Young

  2. Cangene Corporation, Mississauga, ON, Canada

    Doug Cossar

Authors
  1. Soon-Il Yun
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  2. Ahmad R.M. Yahya
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  3. Doug Cossar
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  4. William A. Anderson
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  5. Jeno M. Scharer
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  6. Murray Moo-Young
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Yun, SI., Yahya, A.R., Cossar, D. et al. Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation. Biotechnology Letters 23, 1903–1905 (2001). https://doi.org/10.1023/A:1013798129637

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  • DOI: https://doi.org/10.1023/A:1013798129637

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