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Role Played by Water–Protein Surrounding of Cofactors in Picosecond Electron Transport Steps in Photosynthesis Reaction Centers

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Abstract

Effect of the water–protein environment of cofactors on the rates and efficiency of conversion of light energy into the energy of a photochemical potential in reaction centers of purple bacterium Rhodobacter sphaeroides is studied. The environment is modified by isotopic replacement D2O → H2O or by adding glycerol and dimethyl sulfoxide (DMSO). The replacement D → H is shown to makes no impact on the midpoint potential E m of the electron donor, whereas addition of 70 vol % of glycerol or 35 vol % of DMSO raises E m by 30 and 45 mV, respectively. Rate constants of charge separation k e and electron transport onto quinone k Q remained unchanged following glycerol addition, while deuteration and DMSO addition diminished k e and k Q by two to three times. In addition to the known component with a characteristic time of about 10 ns, a component with a duration of 0.5–0.8 ns appears in the recombination kinetics of charges of deuterated and DMSO-treated preparations of reaction centers. The mechanism of the environment response on the emergence of nonequilibrium states of cofactors is analyzed theoretically. The energy model proposed for primary processes of photosynthesis accounts for the contribution made by the environment in the realization of a highly effective electron transport.

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Authors and Affiliations

  1. Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    V. Z. Pashchenko, N. P. Grishanova, P. P. Knox, B. N. Korvatovskii & A. B. Rubin

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  1. V. Z. Pashchenko
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  2. N. P. Grishanova
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  3. P. P. Knox
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  4. B. N. Korvatovskii
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  5. A. B. Rubin
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Pashchenko, V.Z., Grishanova, N.P., Knox, P.P. et al. Role Played by Water–Protein Surrounding of Cofactors in Picosecond Electron Transport Steps in Photosynthesis Reaction Centers. Russian Journal of Electrochemistry 38, 88–96 (2002). https://doi.org/10.1023/A:1013794529892

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