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Subcellular Localization of Epidermal Growth Factor in Human Parotid Gland

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Abstract

The intracellular distribution of epidermal growth factor was investigated in human parotid gland by immunogold cytochemistry at the electron-microscopy level. Epidermal growth factor immunoreactivity was demonstrated in both acini and ducts. In acinar cells, secretory granules appeared moderately stained, clearly indicating that parotid gland contributes to salivary epidermal growth factor through granule exocytosis. In ductal cells, gold particles were found to decorate numerous cytoplasmic vesicles, particularly abundant in striated duct cells. Since epidermal growth factor reactive vesicles were seen not only at the cellular apex, but nearby lateral plasma membranes as well, it leads to the hypothesis that epidermal growth factor may be discharged both apically into the saliva, and basally into the interstitium.

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Authors and Affiliations

  1. Dipartimento di Citomorfologia, Università di Cagliari, Cittadella Universitaria, 09042, Monserrato (CA), Italy

    Maria S. Lantini, Marco Piludu & Margherita Cossu

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  1. Maria S. Lantini
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  2. Marco Piludu
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  3. Margherita Cossu
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Lantini, M.S., Piludu, M. & Cossu, M. Subcellular Localization of Epidermal Growth Factor in Human Parotid Gland. Histochem J 33, 427–431 (2001). https://doi.org/10.1023/A:1013780028887

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