Abstract
Application of various carbon materials as substrates for immobilizing hydrogenase and creating a hydrogen enzyme electrode is studied. A stable, highly-active electrocatalyst for mediatorless oxidation–evolution of hydrogen is obtained. The electrocatalyst uses hydrogenase from Th. roseopersicina immobilized on an inactive carbon material (based on carbon cloth TVSh-300M) and retains more than 50% of its initial activity after a half year in storage. An equilibrium hydrogen potential is established on enzyme electrodes made of a graphitized cloth and graphite rods with hydrogenase immobilized on them.
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Morozov, S.V., Karyakina, E.E., Zadvornyi, O.A. et al. Bioelectrocatalysis by Hydrogenase Th. Roseopersicina Immobilized on Carbon Materials. Russian Journal of Electrochemistry 38, 97–102 (2002). https://doi.org/10.1023/A:1013746613962
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DOI: https://doi.org/10.1023/A:1013746613962