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Efficient microbial elimination of methanol inhibition for Naproxen resolution by a lipase

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Abstract

To eliminate methanol inhibition of the activity of a lipase, methanotrophic bacteria, which can convert methanol into water and CO2, were introduced to the reaction of enantioselective hydrolysis of Naproxen methyl ester catalysed by lipase from Candida rugosa. Both the activity and stability of lipase were improved by the removal of methanol by the bacteria.

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Authors and Affiliations

  1. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, P.R. China

    Yi Xu, Jianbo Chen, Jiaying Xin, Shuben Li, Chungu Xia & Junru Cui

Authors
  1. Yi Xu
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  2. Jianbo Chen
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  3. Jiaying Xin
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  4. Shuben Li
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  5. Chungu Xia
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  6. Junru Cui
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Corresponding author

Correspondence to Shuben Li.

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Xu, Y., Chen, J., Xin, J. et al. Efficient microbial elimination of methanol inhibition for Naproxen resolution by a lipase. Biotechnology Letters 23, 1975–1979 (2001). https://doi.org/10.1023/A:1013738400504

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  • DOI: https://doi.org/10.1023/A:1013738400504

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