Abstract
Photosynthetic activity of leaf disks from chlortoluron (2 µmol per plant) treated and non-treated non-transgenic and transgenic (PGF-6) tobacco plants was measured from 1 up to 21 d after treatment under greenhouse conditions. PGF-6 plants, expressing the fused rat cytochrome P4501A1/yeast reductase genes were used. PGF-6 plants were much more chlortoluron-resistant than control plants. In non-transgenic tobacco plants the electron transport flow to PQ pool was strongly inhibited 1 d after treatment with herbicide whereas it was still existing in PGF-6 plants although some reduction was observed. The quantum yield of photosystem 2 (ΦPS2) which is related to the quantum yield of whole-chain electron transfer was much more inhibited by chlortoluron than the primary PS2 photochemistry, measured by the ratio Fv/Fm. Lower PS2 activity was found for herbicide-treated non-transgenic plants up to the 9th day. Then it started to increase in both control and PGF-6 plants, but more rapidly in PGF-6 ones, and its values were near to the control level at the 21st d after chlortoluron treatment.
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References
Butler, W.L.: Chlorophyll fluorescence: a probe for electron transfer and energy transfer.-In: Trebst, A., Avron, M. (ed.): Photosynthesis I. Pp. 149-167. Springer-Verlag, Berlin-Heidelberg-New York 1977.
Carter, G.A., Cibula, W.G., Miller, R.L.: Narrow-band reflectance imagery compared with thermal imagery for early detection of plant stress.-J. Plant Physiol. 148: 515-522, 1996.
Fernandez, R., Whitwell, T., Riley, M., Bernard, C.: Evaluating semiaquatic herbaceous perennials for use in herbicide phytoremediation.-J. amer. Soc. hort. Sci. 124: 539-544, 1999.
Foes, M., Liu, L., Vigue, G., Stoller, E., Wax, L., Tranel, P.: A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides.-Weed Sci. 47: 20-27, 1999.
Hall, L., Moss, S., Powles, S.: Mechanisms of resistance to chlorotoluron in two biotypes of the grass weed Alopecurus myosuroides.-Pestic. Biochem. Physiol. 53: 180-192, 1995.
Jensen, M., Linke, K., Dickhauser, A., Feige, G.: The effect of agronomic photosystem II herbicides on lichens.-Lichenologist 31: 95-103, 1999.
Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.-Photosynth. Res. 5: 139-157, 1984.
Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991.
Lichtenthaler, H.K., Rinderle, U.: The role of chlorophyll fluorescence in the detection of stress conditions in plants.-CRC crit. Rev. anal. Chem. 19: S29-S85, 1988.
Ohkawa, H., Hiromasa, I., Shiota, N., Inui, H., Ohkawa, Y.: Molecular mechanisms of herbicide resistance.-Rev. Toxicol. 2: 245-252, 1998.
Ohkawa, H., Shiota, N., Inui, H., Sigiura, M., Yabusaki, Y., Ohkawa, Y., Ishige, T.: Herbicide-resistant tobacco and potato plants expressing mammalian P450 monooxygenases.-In: Hatzios, K.K. (ed.): Regulation of Enzymatic System Detoxifying Xenobiotics in Plants. Pp. 307-312. Kluwer Acad. Publ., Dordrecht 1997.
Papageorgiou, G.: Chlorophyll fluorescence: an intrinsic probe of photosynthesis.-In: Govindjee (ed.): Bioenergetics of Photosynthesis. Pp. 319-371. Academic Press, New York-San Francisco-London 1975.
Rintamäki, E., Salo, R., Aro, E.-M.: Rapid turnover of the D1 reaction-center protein of photosystem II as a protection mechanism against photoinhibition in a moss, Ceratodon purpureus (Hedw.) Brid.-Planta 193: 520-529, 1994.
Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986.
Shiota, N., Inui, I., Ohkawa, H.: Metabolism of the herbicide chlortoluron in transgenic tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 and yeast NADPH-cytochrome P450 oxidoreductase.-Pestic. Biochem. Physiol. 54: 190-198, 1996.
Shiota, N., Nagasawa, A., Sakaki, T., Yabasaki, Y., Ohkawa, H.: Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase.-Plant Physiol. 106: 17-23, 1994.
Tyystjärvi, E., Karunen, J.: A microcomputer program and fast analogy to digital converter card for the analysis of fluorescence induction transients.-Photosynth. Res. 26: 127-132, 1990.
Vidal, D., Martinez-Guijarro, J., Simon, E.: Chlorophyll fluorescence and photosynthetic O2 evolution in Vicia faba plants treated with methabenzthiazuron.-Photosynthetica 31: 9-20, 1995.
Yordanova, E., Gorinova, N., Bachvarova, R., Nedkovska, M., Atanassov, A., Ohkawa, H.: Genetic transformation of tobacco with rat cytochrome P4501A1 monooxygenase gene.-Bulg. J. agr. Sci. 6: 1-7, 2000.
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Yordanova, E., Georgieva, K., Gorinova, N. et al. Influence of the Herbicide Chlortoluron on Photosynthetic Activity in Transgenic Tobacco Plants. Photosynthetica 39, 313–316 (2001). https://doi.org/10.1023/A:1013713511643
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DOI: https://doi.org/10.1023/A:1013713511643