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Stem Cell Factor Expressed in Human Gastric Mucosa in Relation to Mast Cell Increase in Helicobacter pylori-Infected Gastritis

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Abstract

We previously reported mast cell increases in H. pylori gastritis. To determine the mechanism, we investigated the kinetics of mast cells and mast cell growth factor (stem cell factor, SCF) in H. pylori-positive and -negative gastric mucosa. Biopsy specimens from 12 H. pylori-negative and 28 positive subjects were examined. Sections were stained for mast cells, proliferating cell nuclear antigen (PCNA), and SCF. Densities of mast cells, PCNA-positive mast cells, and SCF-positive cells were significantly greater in H. pylori-positive than -negative subjects. SCF was expressed in mast cells and fibroblasts. The density of SCF-positive fibroblasts increased in H. pylori-positive gastritis and decreased after cure of infection. SCF mRNA was detected in H. pylori-positive gastric mucosa. Fibroblasts isolated from the normal gastric mucosa expressed SCF mRNA after incubation with H. pylori water extract. SCF may be one of the factors for mast cell increase. Fibroblasts may participate in mast cell increase and inflammation in H. pylori infection.

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REFERENCES

  1. Nakajima S, Krishnan B, Ota H, Segura AM, Hattori T, Graham DY, Genta RM: Mast cell involvement in gastritis with or without Helicobacter pylori infection. Gastroenterology 114:746–754, 1997

    Google Scholar 

  2. Wallace JL, Keenan CM, Mugridge KG: Possible mechanisms and mediators of gastritis associated with Helicobacter pylori infection. Scand J Gastroenterol 26(suppl 187):65–70, 1991

    Google Scholar 

  3. Bertram TA, Krakowka S, Morgan DR: Gastritis associated with infection by Helicobacter pylori: Comparative pathology in humans and swine. Rev Infect Dis 13(suppl 18):S714–S722, 1991

    Google Scholar 

  4. Megraud F, Lamouliatte H: Helicobacter pylori and duodenal ulcer: Evidence suggesting cautions. Dig Dis Sci 37:769–772, 1992

    Google Scholar 

  5. Nakajima S, Graham DY, Hattori T, Bamba T: Strategy for treatment of Helicobacter pylori infection in adults. I. Updated indications for test and eradication therapy suggested in 2000. Curr Pharm Des 6:1503–1514, 2000

    Google Scholar 

  6. Schleimer RP, Fox CC, Neclerio RM: Role of human basophils and mast cells in the pathogenesis of allergic disease. J Allergy Clin Immunol 76:369–374, 1985

    Google Scholar 

  7. Serafiin WE, Austen KF: Current concepts: Mediators of immediate hypersensitivity reactions. N Engl J Med 317:30–34, 1987

    Google Scholar 

  8. Galli SJ: New concepts about the mast cell. N Engl J Med 328:257–265, 1993

    Google Scholar 

  9. Marshall JS, Bienenstock J: The role of mast cells in inflammatory reactions of the airways, skin and intestine. Curr Opin Immunol 6:853–859, 1994

    Google Scholar 

  10. Goetzl EJ. Derian CK, Tauber AL: Novel effects of 1-Ohexadecyle-2-acyle-sn-glycero-3-phosphorylcholine mediators on human leukocyte function: Delineation of the specific roles of the acyle substituents. Biochem Biophys Res Commun 110:1482–1490, 1980

    Google Scholar 

  11. Nisson G, Butterfield JH, Nissson K, Siegbahn A: Stem cell factor is a chemotacticfactor for human mast cells. J Immunol 153:3717–3123, 1994

    Google Scholar 

  12. Gruber BL, Marchese MJ, Kew RR: Transforming growth factor β-1 mediates mast cell chemotaxis. J Immunol 152:5860–5867, 1994

    Google Scholar 

  13. Saito H, Ebisawa M, Sakaguchi N, Onda T, Iikura Y, Yanagida M, Uzumaki H, Nakahata T: Characterization of cord-blood-derived human mast cells cultured in the presence of steel factor and interleukin 6. Int Arch Allergy Immunol 107:63–65, 1995

    Google Scholar 

  14. Denburg JA: Basophil and mast cell lineages in vitro and in vivo. Blood 79:846–860, 1992

    Google Scholar 

  15. Gionchetti P, Vaira D, Campieri M, Holton J: Enhanced mucosal interleukin 6 and 8 in Helicobacter pylori-positive dyspeptic patients. Am J Gastroenterol 29:425–429, 1994

    Google Scholar 

  16. Tufano MA, Rossano F, Catalanotti P, Liguori G, Capasso C, Ceccarelli MT, Marinelli P: Immunobiological activities of Helicobacter pylori porins. Infect Immun 62:1392–1399, 1994

    Google Scholar 

  17. Grabbe J, Welker P, Dippel E, Czarnetzki BM: Stem cell factor, a novel cutaneous growth factor for mast cells and melanocytes. Arch Dermatol Res 287:78–84, 1994

    Google Scholar 

  18. Nakajima S, Arizono N, Hattori T, Bamba T: Increase in mucosal and connective tissue-type mast cells in the stomach with acetic acid-induced ulcer in rat. APMIS 104:19–29, 1996

    Google Scholar 

  19. Dixon MF, Genta RM, Yardley JH, Correa P: Classification and grading of gastritis. Am J Surg Pathol 20:1161–1181, 1996

    Google Scholar 

  20. Kasai K, Yoshikawa T, Yoshida N, Hashiramoto A, Kondou M, Murase H: Helicobacter pylori water extract induces interleukin-8 production by gastric epitherial cells. Dig Dis Sci 44:237–242, 1999

    Google Scholar 

  21. Zhang S, Anderson DF, Bradding P, Coward WR, Baddeley SM, Macleod JAD, Mcgill JI, Church MK, Holgate ST, Roche WR: J Pathol 186:59–66, 1998

    Google Scholar 

  22. Mai UE, Perez Perez GI, Allen JB: Surface proteins from Helicobacter pylori exhibit chemotactic activity for human leukocytes and are present in gastric mucosa. J Exp Med 175:517–525, 1993

    Google Scholar 

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Authors and Affiliations

  1. Second Department of Internal Medicine and Department of Pathology, Shiga, University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga, 520-2192, Japan

    Nobumichi Bamba & Shigemi Nakajima

  2. Second Department of Internal Medicine, Shiga, University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga, 520-2192, Japan

    Akira Andoh & Tadao Bamba

  3. Department of Pathology, Shiga, University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga, 520-2192, Japan

    Masamichi Bamba, Hiroyuki Sugihara & Takanori Hattori

Authors
  1. Nobumichi Bamba
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  2. Shigemi Nakajima
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  3. Akira Andoh
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  4. Masamichi Bamba
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  5. Hiroyuki Sugihara
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  6. Tadao Bamba
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  7. Takanori Hattori
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Bamba, N., Nakajima, S., Andoh, A. et al. Stem Cell Factor Expressed in Human Gastric Mucosa in Relation to Mast Cell Increase in Helicobacter pylori-Infected Gastritis. Dig Dis Sci 47, 274–282 (2002). https://doi.org/10.1023/A:1013701618079

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