Plant and Soil

, Volume 237, Issue 1, pp 47–54 | Cite as

Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro

  • M. Sajjad Mirza
  • Waseem Ahmad
  • Farooq Latif
  • Jacqueline Haurat
  • Rene Bally
  • Philippe Normand
  • Kauser A. Malik


We report the isolation of nitrogen fixing, phytohormone producing bacteria from sugarcane and their beneficial effects on the growth of micropropagated sugarcane plantlets. Detection of the nitrogen fixing bacteria by ARA-based MPN (acetylene reduction assay-based most probable number) method indicated the presence of up to 106 bacteria per gram dry weight of stem and 107 bacteria per gram dry weight of root of field-grown sugarcane. Two nitrogen fixing bacterial isolates were obtained from stem (SC11, SC20) and two from the roots (SR12, SR13) of field-grown plants. These isolates were identified as Enterobacter sp. strains on the basis of their morphological characteristics and biochemical tests. The isolate SC20 was further characterized by 16S rRNA sequence analysis, which showed high sequence similarity to the sequence of Enterobacter cloacae and Klebsiella oxytoca. All the isolates produced the phytohormone indoleacetic acid (IAA) in pure culture and this IAA production was enhanced in growth medium containing tryptophan. The bacterial isolates were used to inoculate micro-propagated sugarcane in vitro where maximum increase in the root and shoot weight over control was observed in the plantlets inoculated with strain SC20. By using the15N isotope dilution technique, maximum nitrogen fixation contribution (28% of total plant nitrogen) was detected in plantlets inoculated with isolate SC20.

Enterobacter nitrogen fixation phytohormones 16S rRNA 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • M. Sajjad Mirza
    • 1
  • Waseem Ahmad
    • 1
  • Farooq Latif
    • 1
  • Jacqueline Haurat
    • 2
  • Rene Bally
    • 2
  • Philippe Normand
    • 2
  • Kauser A. Malik
    • 2
  1. 1.Biofertilizer Division, National Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan
  2. 2.Laboratoire d'Ecologie Microbienne du Sol, UMR CNRS 5557, UCB Lyon1Villeurbanne CedexFrance

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