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A Specific DNA-Dependent DNA Polymerase Is Associated with Saccharomyces cerevisiae Telomerase

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Abstract

The telomere DNA of most eucaryotes consists of tandem DNA repeats and a number of associated proteins. The synthesis of the G-rich DNA strand is performed by the telomerase complex. The complementary C-strand is synthesized by DNA-dependent DNA polymerases. Using telomerase reverse transcriptase tagging followed by immunoprecipitation cou pled with two-step purification, we have found a specific DNA-dependent DNA polymerase activity associated with telom erase of Saccharomyces cerevisiae. Investigation of the biochemical properties of this DNA polymerase activity confirms the hypothesis of tight interaction of DNA polymerase δ with telomerase.

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Authors and Affiliations

  1. School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia

    A. V. Petrov, P. V. Dmitriev & O. A. Dontsova

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117312, Russia

    K. A. Sokolov

Authors
  1. A. V. Petrov
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  2. P. V. Dmitriev
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  3. K. A. Sokolov
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  4. O. A. Dontsova
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Petrov, A.V., Dmitriev, P.V., Sokolov, K.A. et al. A Specific DNA-Dependent DNA Polymerase Is Associated with Saccharomyces cerevisiae Telomerase. Biochemistry (Moscow) 66, 1361–1367 (2001). https://doi.org/10.1023/A:1013381712357

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