Molecular Breeding

, Volume 8, Issue 2, pp 119–127 | Cite as

Pathogen-derived transgenic resistance to soybean mosaic virus in soybean

  • Xinyu Wang
  • Alan L. Eggenberger
  • Forrest W. NutterJr.
  • John H. Hill
Article

Abstract

Development of transgenic disease resistance in soybeans, despite progress in other important crop plants, has advanced slowly. In this study, transgenic soybean plants resistant to soybean mosaic virus (SMV) were obtained by transforming with the coat protein gene and the 3′-UTR from SMV. Four insertion events were detected in a T0 plant obtained by using Agrobacterium tumefaciens-mediated transformation. Self-pollination of T0 progeny yielded four homozygous transgenic lines with a single insertion event or combinations of two insertion events in the T3 generation. A single coat protein gene transcript was detected in all four transgenic lines, and virus coat protein was detected in three transgenic lines. Two transgenic lines were highly resistant to the virus. These constitute the first example of stable genetically engineered disease resistance in soybean.

Pathogen-derived resistance Soybean mosaic virus Soybean transformation 

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References

  1. Altenbach S.B., Pearson K.W., Meeker G., Staraci L.C. and Sun S.S.M. 1989. Enhancement of methionine content of seed proteins by expression of a chimeric gene encoding a methioninerich protein in transgenic plants. Plant Mol. Biol. 13: 513–522.Google Scholar
  2. Barnett O.W. 1992. Potyvirus taxonomy. Arch. Virol. Supplem. 5: 435–450.Google Scholar
  3. Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.Google Scholar
  4. Brakke M.K. and van Pelt N. 1970. Properties of infectious ribonucleic acid from wheat streak mosaic virus. Virology 42: 699–706.Google Scholar
  5. Bryant G.R., Hill J.H., Bailey T.B., Tachibana H., Durand D.P. and Benner H.I. 1982. Detection of soybean mosaic virus in seed by solid-phase radioimmunoassay. Plant Dis. 66: 693–695.Google Scholar
  6. Buzzel R.I. and Tu J.C. 1984. Inheritance of soybean resistance to soybean mosaic virus. J. Hered. 75: 82.Google Scholar
  7. Cervera M.T., Reichmann J.L., Martin M.T. and Garcia J.A. 1993. 3′-terminal sequence of the plum pox virus PS and 06 isolates: evidence for RNA recombination within the potyvirus group. J. Gen. Virol. 74: 329–334.Google Scholar
  8. Cho E.K. and Goodman R.M. 1979. Strains of soybean mosaic virus: classification based on virulence in resistant soybean cultivars. Phytopathology 69: 467–470.Google Scholar
  9. Cho E.K., Chung B.J. and Lee S.H. 1977. Studies on identification and classification of soybean virus disease in Korea. II. Etiology of a necrotic disease of Glycine max. Plant Dis. Rep. 61: 313–317.Google Scholar
  10. Chomczynski P. and Mackey K. 1994. One-hour downward capillary blotting of RNA at neutral pH. Anal. Biochem. 221: 303–305.Google Scholar
  11. Demski J.W. and Jellum M.D. 1975. Single and double virus infection of soybean: plant characteristics and chemical composition. Phytopathology 65: 1154–1156.Google Scholar
  12. Dhingra K.L. and Chenulu V.V. 1980. Effect of soybean mosaic virus on yield and nodulation of soybean cv. Bragg. Ind. Phytopath. 33: 586–590.Google Scholar
  13. Di R., Purcell V., Collins G.B. and Ghabrial S.A. 1996. Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene. Plant Cell Rep. 15: 746–750.Google Scholar
  14. Dougherty W.G. and Carrington J.C. 1988. Expression and function of potyviral gene products. Annu. Rev. Phytopath. 26: 123–143.Google Scholar
  15. Eggenberger A.L., Stark D.M. and Beachy R.N. 1989. The nucleotide sequence of a soybean mosaic virus coat protein-coding region and its expression in Escherichia coli, Agrobacterium tumefaciens and tobacco callus. J. Gen. Virol. 70: 1853–1860.Google Scholar
  16. El-Amrety A.A., El-Said H.M. and Salem D.E. 1985. Effect of soybean mosaic virus infection on quality of soybean seed. Agric. Res. Rev. 63: 155–164.Google Scholar
  17. Fehr W.R. and Caviness C.E. 1977. Stages of soybean development. Iowa Agric. Home Econ. Exp. Stn. Spec. Rep. 80: 1–12.Google Scholar
  18. Fitchen J.H. and Beachy R.N. 1993. Genetically engineered protection against viruses in transgenic plants. Annu. Rev. Microbiol. 47: 739–763.Google Scholar
  19. Gal-On A., Antignus Y., Rosner A. and Raccah B. 1992. A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication. J. Gen.Virol. 73: 2183–2187.Google Scholar
  20. Hari V. 1981. The RNA of tobacco etch virus: further characterization and detection of protein linked to RNA. Virology 112: 391–399.Google Scholar
  21. Hill J.H. and Benner H.I. 1980. Properties of soybean mosaic virus and its isolated protein. Phytopath. Z. 97: 272–281.Google Scholar
  22. Hill J.H. and Durand D.P 1986. Soybean mosaic virus. In: Bergmeyer H.U. (ed.), Methods of Enzymatic Analysis. VCH Verlagsgesellschaft, Weinheim, Germany, pp. 455–474.Google Scholar
  23. Hill J.H., Lucas B.S., Benner H.I., Tachibana H., Hammond R.B. and Pedigo L.P. 1980. Factors associated with the epidemiology of soybean mosaic virus in Iowa. Phytopathology 70: 536–540.Google Scholar
  24. Hill J.H., Benner H.I., Permar T.A., Bailey T.B., Andrews R.E., Durand D.P. and van Deusen R.A. 1989. Differentiation of soybean mosaic virus isolates by one-dimensional trypsin peptide maps immunoblotted with monoclonal antibodies. Phytopathology 79: 1261–1265.Google Scholar
  25. Irwin M.E. and Goodman R.M. 1981. Ecology and control of soybean mosaic virus. In: Maramorosch K. and Harris K.F. (eds.), Plant Diseases and Their Vectors: Ecology and Epidemiology. Academic Press, New York, pp. 181–220.Google Scholar
  26. Jayaram Ch., Hill J.H. and Miller W.A. 1992. Complete nucleotide sequences of two soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. J. Gen. Virol. 73: 2067–2077.Google Scholar
  27. Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.Google Scholar
  28. Lain S., Riechmann J.L., Mendez E. and Garcia J.A. 1988. Nucleotide sequence of the 3′ terminal region of plum pox potyvirus RNA. Virus Res. 10: 325–342.Google Scholar
  29. Lim S.M. 1985. Resistance to soybean mosaic virus in soybeans. Phytopathology 75: 199–201.Google Scholar
  30. Logemann J., Schell J. and Willmitzer L. 1987. Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163: 16–20.Google Scholar
  31. Lomonosoff G.P. 1995. Pathogen-derived resistance to plant viruses. Annu. Rev. Phytopath. 33: 323–343.Google Scholar
  32. Nutter F.W. Jr., Schultz P.M. and Hill J.H. 1998. Quantification of within-field spread of soybean mosaic virus in soybean using strain-specific monoclonal antibodies. Phytopathology 88: 895–901.Google Scholar
  33. Padgette S.R., Kolacz K.H., Delannay X., Re D.B., LaValle B.J., Richolz D.A., Peschke V.M., Nida D.L., Taylor N.B. and Kishore G.M. 1995. Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci. 35: 1451–1461.Google Scholar
  34. Powell-Abel P., Nelson R.S., De B., Hoffmann N., Rogers S.G., Fraley R.T. and Beachy R.N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.Google Scholar
  35. Revers F., Le Gall O., Candresse T., Le Romancer M. and Dunez J. 1996. Frequent occurrence of recombinant potyvirus isolates. J. Gen. Virol. 77: 1953–1965.Google Scholar
  36. Ross J.P. 1969. Effect of time and sequence of inoculation of soybean with soybean mosaic and bean pod mottle viruses on yields and seed characters. Phytopathology 59: 1404–1408.Google Scholar
  37. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A. and Allard R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA. 81: 8014–8018.Google Scholar
  38. Sanford J.D. and Johnson S.A. 1985. The concept of parasitederived resistance. J. Theor. Biol. 115: 395–405.Google Scholar
  39. Shah D.M., Rommens C.M.T. and Beachy R.N. 1995. Resistance to diseases and insects in transgenic plants: progress and applications to agriculture. Trends Biotechnol. 13: 362–368.Google Scholar
  40. Townsend J.A. and Thomas L.A. 1996. Method of Agrobacteriummediated transformation of cultured soybean cells. U.S. Patent 5563055.Google Scholar
  41. Waterhouse P.M., Smith N.A. and Wang M.-B. 1999. Virus resistance and gene silencing: killing the messenger. Trends Plant Sci. 4: 452–457.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Xinyu Wang
    • 1
  • Alan L. Eggenberger
    • 1
  • Forrest W. NutterJr.
    • 1
  • John H. Hill
    • 1
  1. 1.Department of Plant PathologyIowa State UniversityAmesUSA

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