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Monoclonal Antibodies to Vascular Endothelial Growth Factor (VEGF) And the VEGF Receptor, FLT-1, Inhibit the Growth of C6 Glioma in a Mouse Xenograft

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Abstract

Monoclonal antibodies raised to peptide sequences of vascular endothelial growth factor (VEGF) and the VEGF receptor, FLT-1, inhibited the growth of C6 tumors growing subcutaneously in nude mice. Immunohistochemical analysis demonstrated antibody targeting of blood vessels, tumor cells, and macrophages. A control antibody demonstrated no growth inhibition or tumor uptake. An antibody to FLT-1 impaired microvascular maturation and diminished the accumulation of tumor infiltrating macrophages. The antibodies demonstrated affinity for microvasculature and tumor cells in immunohistochemistry of human glioblastoma multiforme. Targeting VEGF and its receptors has potential in the treatment of tumors of the central nervous system. FLT-1 presents an attractive target due to its presence on multiple cell types.

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Authors and Affiliations

  1. Department of Radiation Oncology, Laurel Highlands Cancer Program, USA

    David F. Stefanik, Laila R. Rizkalla & Paulette P. Stefanik

  2. Department of Neurological Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Wendy K. Fellows & William C. Welch

  3. Department of Pathology, Conemaugh Memorial Medical Center, Johnstown

    Waheeb M. Rizkalla

  4. University of Pittsburgh Cancer Institute, USA

    Albert B. Deleo

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  1. David F. Stefanik
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Stefanik, D.F., Fellows, W.K., Rizkalla, L.R. et al. Monoclonal Antibodies to Vascular Endothelial Growth Factor (VEGF) And the VEGF Receptor, FLT-1, Inhibit the Growth of C6 Glioma in a Mouse Xenograft. J Neurooncol 55, 91–100 (2001). https://doi.org/10.1023/A:1013329832067

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