Abstract
A method for determination of a non-methylated deoxycytidine (dC) residue in the recognition site of 5-cytosine DNA-methyltransferases is suggested. The method is based on treatment of methylated DNA by sodium bisulfite and successive reaction of the thus modified DNA with a repair enzyme, uracil-DNA glycosylase. This method was successfully applied to identify NlaX methyltransferase specificity.
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Kubareva, E.A., Walter, J., Vorob'eva, O.V. et al. Determination of a Non-methylated Deoxycytidine Residue in the Recognition Site of DNA-methyltransferases. Biochemistry (Moscow) 66, 1356–1360 (2001). https://doi.org/10.1023/A:1013329728287
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DOI: https://doi.org/10.1023/A:1013329728287