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Corticosteroid-binding globulin synthesis and distribution in rat white adipose tissue

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Abstract

Corticosterone binding (CB) capacity was determined in visceral and subcutaneous white adipose tissue (WAT), as well as in plasma of lean Zucker rats. Perfusion of rats with saline eliminated most liver and kidney corticosterone binding but did not affect CB in WAT. The cytosol extracts of isolated cells, however, did not bind corticosterone in detectable amounts. By means of a RT-PCR procedure it was found that corticosterone-binding globulin (CBG) was expressed in WAT. By immunohistochemical detection in WAT sections, CBG was seen in a thin layer surrounding the cells near the plasma membrane. These data suggest that the CBG layer surrounding the cells may act as a protective barrier limiting the access of glucocorticoids to adipocytes.

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Authors and Affiliations

  1. Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain

    Maria del Mar Grasa, Cristina Cabot, Cristina Adán, Montserrat Esteve, José Antonio Fernández-López, Xavier Remesar & Marià Alemany

  2. Institute of Normal Morphology-Anatomy, University of Ancona, Ancona, Italy

    Rita de Matteis & Saverio Cinti

Authors
  1. Maria del Mar Grasa
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  2. Cristina Cabot
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  3. Cristina Adán
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  4. Rita de Matteis
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  5. Montserrat Esteve
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  6. Saverio Cinti
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  7. José Antonio Fernández-López
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  8. Xavier Remesar
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  9. Marià Alemany
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Correspondence to Marià Alemany.

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Grasa, M.d.M., Cabot, C., Adán, C. et al. Corticosteroid-binding globulin synthesis and distribution in rat white adipose tissue. Mol Cell Biochem 228, 25–31 (2001). https://doi.org/10.1023/A:1013304223967

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  • DOI: https://doi.org/10.1023/A:1013304223967

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