Abstract
We developed a novel model for studying migration of keratinocyte colonies over 3D collagen gel. It was shown that keratinocytes previously cultured on plastic and then seeded on the surface of collagen gel proliferate more actively than the primary cells. The gel density could significantly affect the morphology and migration of keratinocyte colonies. Modification of collagen gel by fibronectin-like protein or poly-L-lysine altered the morphometric parameters of colonies without significant changes in the velocity of migration.
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REFERENCES
Assouline, M., Chew, S.J., Thompson, H.W., and Beuerman, R., Effect of Growth Factors on Collagen Lattice Contraction by Human Keratocytes, Invest. Ophthalmol. Visual Sci., 1992, vol. 33, no. 5, pp. 1742-1755.
Bell, E., Ehrlich, H. P., Sher, S., et al., Development and Use of a Living Skin Equivalent, J. Plastic Rec. Surg., 1981, vol. 67, no. 3, pp. 386-392.
Bell, E., Parenteau, N., Gay, R., et al., The Living Skin Equivalent: Its Manufacture, Its Organotypic Properties and Its Responses to Irritants, Toxic. In Vitro, 1991, vol. 5, pp. 591-596.
Choquet, D., Felsenfeld, D.P., and Sheetz, M.P., Extracellular Matrix Rigidity Causes Strengthening of Integrin-Cytoskeleton Linkages, Cell, 1997, vol. 88, pp. 39-48.
Dunn, G.A. and Brown, A.F., Alignment of Fibroblasts on Grooved Surfaces Described by Simple Geometric Transformation, J. Cell Sci., 1986, vol. 83, pp. 313-340.
Emerman, J. and Pitelka, D., Maintenance and Induction of Morphological Differentiation in Dissociated Mammary Epithelium on Floating Collagen Membranes, in vitro, 1977, vol. 13, no. 5, pp. 316-322.
Fluck, J., Querfeld, C, Cremer, A., et al., Normal Human Primary Fibroblasts Undergo Apoptosis in Three-Dimensional Contractile Collagen Gels, J. Invest. Dermatol., 1998, vol. 110, pp. 153-157.
Grinnell, F., The Activated Keratinocyte: Up-Regulation of Cell Adhesion and Migration during Wound Healing, J. Trauma, 1990, vol. 30, no. 12, pp. S144-S149.
Grinnell, F., Toda, K-I., and Lamke-Seymour, C., Reconstitution of Human Epidermis in vitro Is Accompanied by Transient Activation of Basal Keratinocyte Spreading, Exp. Cell Res., 1987, vol. 172, pp. 439-449.
Guo, M., Toda, K.-I., and Grinnell, F., Activation of Human Keratinocyte Migration on Type I Collagen and Fibronectin, J. Cell Sci., 1990, vol. 96, pp. 197-205.
Hirai, Y., Lochter, A., Galosy, S., et al., Epimorphine Functions as a Key Morphoregulator for Mammary Epithelial Cells, J. Cell Biol., 1998, vol. 140, no. 1, pp. 159-169.
Kim, L.T., Ishihara, S., Lee, C.-C., et al., Altered Glycosylation and Cell Surface Expression of β1 Integrin Receptors during Keratinocyte Activation, J. Cell Sci., 1992, vol. 103, pp. 743-754.
Lee, J., Leonard, M., Oliver, T., iet al., Traction Forces Generated by Locomoting Keratinocytes, J. Cell Biol., 1994, vol. 127, pp. 1957-1964.
Mertz, P.M., Davis, S.C., Franzen, L., et al., Effects of Arginine-Glycine-Aspartic Acid Peptide-Containing Artificial Matrix on Epithelial Migration in vitro and Experimental Second-Degree Burn Wound Healing in vivo, J. Burn Care Rehabil., 1996, vol. 17 (3), pp. 199-206.
Mourra, N., Borderie, V., and Laroche, L., Ultrastructural and Immunohistochemical Study of 3-Dimensional Cultures of Human Keratinocytes on a Collagen Gel, J. Fr. Ophthalmol., 1998, vol. 21, no. 4, pp. 287-294.
Nanchahal, J. and Davies, D., Cultured Composite Skin Grafts. Banked Allografts Offer a Prospect of Immediate Wound Coverage, Brit. Med. J., 1990, vol. 301, pp. 1342-1343.
Palecek, S.P., Loftus, J.C., Ginsberg, M.H., et al., Integrin-Ligand Binding Properties Govern Cell Migration Speed through Cell-Substratum Adhesiveness, Nature, 1997, vol. 385, pp. 537-540.
Saga, K. and Takahashi, M., Localization of Anionic Sites in Normal and Psoriatic Epidermis: The Effect of Enzyme Digestion on These Anionic Sites, Br. J. Dermatol., 1995, vol. 132, no. 5, pp. 710-717.
Sarret, Y., Stamm, C., Jullien, D., and Schmitt, D., Keratinocyte Migration Is Partially Supported by the Cell-Binding Domain of Fibronectin and Is RGDS-Dependent, J. Invest. Dermatol., 1992, vol. 99, no. 5, pp. 656-659.
Schuler, G., Pohlin, G., and Fritsch, P.O., Differences of Cell Surface Label Distribution and Redistribution Patterns between Mammalian Keratinocytes and Melanocytes in Culture, J. Invest. Dermatol., 1981, vol. 77, no. 4, pp. 347-352.
Tsai, R.J.-F., Ho, Y.-S., and Chen, J.-K., The Effects of Fibroblasts on the Growth and Differentiation of Human Bulbar Conjunctival Epithelial Cells in an in vitro Conjunctival Equivalent, Invest. Ophthalmol. Visual Sci., 1994, vol. 35, no. 6, pp. 2865-2875.
Vasiliev, A.V., Voloshin, A.V., and Terskikh, V.V., Role of Feeder Cells in Fixation and Growth of Human and Rat Keratinocytes, Tsitologiya, 1991, vol. 33, no. 12, pp. 84-89.
Vasiliev, A.V., Voloshin, A.V., Vorotelyak, E.A., and Terskikh, V.V., Migration of Colonies of Human Epidermal Keratinocytes in Culture, Dokl. Ross. Akad. Nauk, 1993, vol. 329, no. 2, pp. 232-235.
Vorotelyak, E.A., Migration of Human Epidermal cells in Culture, Cand. Sci. (Biol.) Dissertation, Moscow, 1998.
Vorotelyak, E.A., Leonova, O.G., Shinin, V.V., et al., Growth of Human Epidermal Keratinocytes on Collagen Gel, Dokl. Ross. Akad. Nauk, 1999, vol. 369, no. 5.
Weinberg, C.B. and Bell, E., A Blood Vessel Model Constructed from Collagen and Cultured Vascular Cells, Science, 1986, vol. 231, no. 4736, pp. 397-400.
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Vorotelyak, E.A., Shikhverdieva, A.S., Vasiliev, A.V. et al. Simulation of Migration of Human Epidermal Keratinocytes over Three-Dimensional Collagen Gel. Biology Bulletin 29, 24–30 (2002). https://doi.org/10.1023/A:1013233617151
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DOI: https://doi.org/10.1023/A:1013233617151