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Digestive Diseases and Sciences

, Volume 47, Issue 1, pp 208–215 | Cite as

Physical and Psychological Stress in Rats Enhances Colonic Epithelial Permeability via Peripheral CRH

  • Paul R. Saunders
  • Javier Santos
  • Nico P.M. Hanssen
  • Derrick Yates
  • Jack A. Groot
  • Mary H. Perdue
Article

Abstract

Stress may be a contributing factor in intestinal inflammatory disease; however, the underlying mechanisms have not been elucidated. We previously reported that acute stress altered jejunal epithelial physiology. In this study, we examined both physical and psychological stress-induced functional changes in colonic mucosa. Colonic mucosal tissue from rats subjected to either 2 hr of cold-restraint stress or 1 hr of water-avoidance stress demonstrated altered ionic transport as well as significantly elevated baseline conductance (ionic permeability) and flux of horseradish peroxidase (macromolecular permeability). Intraperitoneal pretreatment with the corticotropin-releasing hormone (CRH) antagonist, α helical CRH9–41, inhibited the stress-induced abnormalities, while exogenous intraperitoneal administration of CRH, to control rats, mimicked the stress responses and in vitro CRH increased the macromolecular permeability. These results suggest that peripheral CRH mediates stress-induced colonic pathophysiology. We speculate that a stress-induced barrier defect may allow uptake of immunogenic substances into the colonic mucosa, initiating or exacerbating intestinal inflammation.

colonic mucosa epithelium permeability stress corticotropin-releasing hormone 

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Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Paul R. Saunders
    • 1
  • Javier Santos
    • 1
  • Nico P.M. Hanssen
    • 2
  • Derrick Yates
    • 1
  • Jack A. Groot
    • 2
  • Mary H. Perdue
    • 1
  1. 1.Intestinal Disease Research Program, Department of Pathology and Molecular Medicine, Faculty of Health SciencesMcMaster UniversityHamiltonCanada
  2. 2.Institute of NeurobiologyUniversity of AmsterdamAmsterdamNetherlands

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