Abstract
Vibrational IR-emission spectra of bacteriorhodopsin (bR) were recorded under continuous illumination with visible light at room temperature. They contain selective information about the chromophore, Schiff base, and opsin. The spectral bands were identified by comparing the data with resonance Raman and IR absorption data. The IR-emission spectra were shown to contain a set of bands characteristic for both all-trans (bR568) and 13-cis conformations (K610-like intermediate) simultaneously. Variation of spectral composition and the intensity of visible light illumination influenced the spectral traces and intensity distribution between them. Greater intensity of deformational vibrations suggests distorted retinal structure in the vibrationally excited ground electronic state. The origin of the emitting species of bR is discussed.
Similar content being viewed by others
REFERENCES
Stoeckenius, W., Lozier, R. H., and Bogomolni, R. A. (1979) Biochim. Biopys. Acta, 505, 215–278.
Mathies, R. A., Lin, S. W., Ames, J. B., and Pollard, W. T. (1991) Annu. Rev. Biophys. Biophys. Chem., 20, 491–518.
Lanyi, J. K. (1992) J. Bioenerg. Biomembr., 24, 169–179.
Govinjee, R., Balashov, S. P., and Ebrey, T. G. (1990) Biophys. J., 58, 597–608.
Lozier, R. H., Bogomolni, R. A., and Stoeckenius, W. (1975) Biophys. J., 15, 614–622.
Braiman, M. A., and Mathies, R. A. (1982) Proc. Natl. Acad. Sci. USA, 79, 403–407.
DeBlase, F. J., and Compton, S. (1991) Appl. Spectrosc., 45, 611–622.
Mink, J., and Keresztury, G. (1993) Appl. Spectrosc., 47, 1446–1459.
Willis, H. A., van der Maas, J. H., and Miller, R. G. J. (eds.) (1987) Laboratory Methods in Vibrational Spectroscopy, J. Willey and Sons, New York.
Terpugov, E. L., and Degtyareva, O. V. (2000) Proc. SPIE, 4129, 97–81.
Terpugov, E. L., and Degtyareva, O. V. (2001) J. Mol. Struct., 565/566, 287–292.
Oesterhelt, D., and Stoechenius, W. (1974) Meth. Enzymol., 31, 667.
Balashov, A. A., Vagin, V. A., Viskovatich, A. V., Lazarev, Yu. A., Terpugov, E. L., and Grishkovshi, G. A. (1991) Proc. SPIE, 1575, 182–183.
Terpugov, E. L., Viskovatykh, A. V., Degtyareva, O. V., and Fesenko, E. E. (1998) Biofizika, 43, 1002–1011.
Genzel, L. L., and Kuhl, J. (1978) Infrared Phys., 18, 113–120.
Aton, B., Doukas, A. G., Callender, R. H., Becher, B., and Ebrey, T. G. (1977) Biochemistry, 16, 2995–2999.
Lewis, A., Spoonhower, J. P., Bogomolni, R. A., Lozier, R. H., and Stoeckenius, W. (1974) Proc. Natl. Acad. Sci. USA, 76, 4462–4466.
Smith, S. O., Braiman, M. S., Myers, A. B., Pardoen, J. A., Courtin, J. M. L., Winkel, C., Lugtenburg, J., and Mathies, R. A. (1987) J. Am. Chem. Soc., 109, 3108–3125.
Terner, J., Hsieh, C.-L., Burns, A. R., and El-Sayed, M. A. (1979) Proc. Natl. Acad. Sci. USA, 76, 3046–3050.
Dioumaev, A. K., and Braiman, M. S. (1997) J. Phys. Chem. B, 101, 1655–1662.
Hage, W., Kim, M., Frei, H., and Mathies, R. A. (1996) J. Chem. Phys., 100, 16026–16033.
Smith, S. J., Pardoen, J. A., Lugtenburg, J., and Mathies, R. A. (1987) J. Am. Chem. Soc., 91, 804–819.
Van den Berg, R., Jang, Du-J., Bitting, H. C., and El-Sayed, M. (1990) Biophys. J., 58, 135–141.
Siebert, F., and Mantele, W. (1983) Eur. J. Biochem., 130, 565–573.
Bagley, K., Dollinger, G., Eisenstein, L., Singh, A. K., and Zimanyi, L. (1982) Proc. Natl. Acad. Sci. USA, 79, 4972–4976.
Smith, S. O., Pardoen, J. A., Mulder, P. P. J., Curry, B., Lugtenburg, J., and Mathies, R. A. (1983) Biochemistry, 22, 6141–6148.
Curry, B., Palings, H., Brock, A. D., Padroen, J. A., Lughenburg, J., and Mathies, R. A. (1985) in Advances in Infrared and Raman Spectroscopy (Clark, R. J. H., and Hester, R. E., eds.) Vol. 12, J. Willey and Sons, N. Y., pp. 115–177.
Ormos, P. (1991) Proc. Natl. Acad. Sci. USA, 88, 473–477.
Clareda, J., Sabes, M., and Padros, E. (1993) Biochemistry, 31, 12363–12368.
Gerwert, K., Hess, B., and Engelhard, M. (1990) FEBS Lett., 261, 449–454.
Rothshild, K., Gray, D., Mogi, T., Marti, T., Braiman, M. A., Stern, L. G., and Khorana, H. G. (1989) Biochemistry, 28, 7052–7059.
Rothshild, K. J., He, Y. W., Gray, D., Roepe, P., Pelltien, S. L., Brown, R. S., and Herzfeld, J. (1987) Proc. Natl. Acad. Sci. USA, 84, 5221–5225.
Gerwert, K., Hess, B., Soppa, J., and Oesterhelt, D. (1989) Proc. Natl. Acad. Sci. USA, 86, 4943–4949.
Zhou, F., Windemuth, A., and Schulten, K. (1993) Biochemistry, 32, 2291–2306.
Alfano, R. R., Govindjee, R., Becker, B., and Ebrey, T. G. (1976) Biophys. J., 16, 541–545.
Sineshchekov, V. A., and Litvin, F. F. (1976) Biofizika, 21, 313–320.
Dencher, N., and Wilms, M. (1975) Biophys. Struct. Mechanism, 1, 259–271.
Kouyma, T., Bogomolni, R. A., and Stoeckenius, W. (1985) Biophys. J., 48, 201–208.
Balashov, S. P., and Litvin, F. F. (1981) Biofizika, 26, 557–568.
Sineshchekov, V. A., Balashov, S. P., and Litvin, F. F. (1981) Biofizika, 26, 964–972.
Terpugov, E. L., and Degtyareva, O. V. (2001) Pisma v ZhETF (Moscow), 6, 320–323.
Balashov, S. P. (1995) Isr. J. Chem., 35, 415–428.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Terpugov, E.L., Degtyareva, O.V. FTIR Emission Spectra of Bacteriorhodopsin in a Vibrational Excited-State. Biochemistry (Moscow) 66, 1315–1322 (2001). https://doi.org/10.1023/A:1013195605416
Issue Date:
DOI: https://doi.org/10.1023/A:1013195605416