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A gene located at 71F in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2

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Abstract

Drosophila melanogaster protein kinase CK2 (DmCK2) is a Ser/Thr protein kinase composed of catalytic α and regulatory β subunits associated as an α2α2 heterotetramer. Using the two-hybrid system, we have screened a Drosophila embryo cDNA library in order to identify proteins that interact with DmCK2α. One of these cDNAs encodes a novel previously undescribed zinc-finger protein, which we call ZFP47. ZFP47 interacts with DmCK2α but not with DmCK2β, indicating that this interaction is specific for the catalytic subunit of CK2. In situ hybridization to polytene chromosomes indicates that the corresponding gene is located at the 72A interval of chromosome III. Sequence analysis indicates that ZFP47 contains a consensus site for phosphorylation by CK2, 4 C2H2-type zinc-fingers, and a bipartite nuclear localization signal. Consistent with the prediction of a site for phosphorylation by CK2, we demonstrate that ZFP47 is phosphorylated by CK2 purified from Drosophila embryos. These studies demonstrate that ZFP47 is a new physiological partner and substrate of CK2.

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Authors and Affiliations

  1. Department of Biology, West Virginia University, Morgantown, WV, 26506, USA

    Madhavi Kalive & Regina L. Trott

  2. Department of Biology and Mary Babb Randolph Cancer Center, West Virginia University, P.O. Box 6057, Brooks Hall, Morgantown, WV, 26506-6057, USA

    A.P. Bidwai

Authors
  1. Madhavi Kalive
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  2. Regina L. Trott
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  3. A.P. Bidwai
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Correspondence to A.P. Bidwai.

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Kalive, M., Trott, R.L. & Bidwai, A. A gene located at 71F in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2. Mol Cell Biochem 227, 99–105 (2001). https://doi.org/10.1023/A:1013185528298

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