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Characterization of Aspergillus sydowii (Thom et Church), a fungal pathogen of Caribbean sea fan corals

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Abstract

In the Caribbean, the fungus Aspergillus sydowii is currently causing an epizootic among sea fan corals (Gorgonia spp.). To elucidate potential factors that may have facilitated the emergence of this disease, we characterized and compared temperature requirements, susceptibility to coral crude extracts, and metabolic profiles of pathogenic (marine) and non-pathogenic (terrestrial) strains of A. sydowii. Growth of all A. sydowii strains were observed at all temperatures tested (22–36 °C) with an optimum of approximately 30 °C. Sea fan crude extracts inhibited growth of A. sydowii but were less effective at higher temperatures. Thus, temperature is likely to have a strong influence on the dynamics of the Gorgonia–Aspergillus interaction by promoting the growth of the pathogen while reducing the efficacy of host resistance. Metabolically, marine A. sydowii strains pathogenic to sea fans were distinct from non-pathogenic terrestrial strains.

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References

  • Abrell, L. M., B. Borgeson & P. Crews, 1996. Chloro polyketides from the cultured fungus (Aspergillus) separated from a marine sponge. Tet. Let. 37: 2331–2333.

    Google Scholar 

  • Antonius, A., 1981. The ‘band’ diseases in coral reefs. Int. coral reef Symp. 2: 7–14.

    Google Scholar 

  • Aronson, R. B. & W. E. Precht, 1997. Stasis, biological disturbance and community structure of a Holocene coral reef. Paleobiology 23: 326–346.

    Google Scholar 

  • Arkoosh, M. R., E. Casillas, E. Clemons, A. N. Kagley, R. Olson, P. Reno & J. E. Stein, 1998. Effect of pollution on fish diseases: Potential impacts on salmonid populations. J. Aquat. Anim. Health 10: 182–190.

    Google Scholar 

  • Belofsky, G., P. R. Jensen, M. K. Renner & W. Fenical, 1998. New cytotoxic sesquiterpenoid nitrobenzoyl esters from a marine strain of the fungus Aspergillus versicolor. Tetrahedron 54: 1715–1724.

    Google Scholar 

  • Bochner, B. R., 1989. Sleuthing out bacterial identities. Nature 339: 157–158.

    Google Scholar 

  • Brown, B. E., 1997. Coral bleaching: causes and consequences. Coral Reefs. 16: S129–138.

    Google Scholar 

  • Carlton, R. G. & L. L. Richardson, 1995. Oxygen and sulfide dynamics in a horizontally migrating cyanobacterial mat: black band disease of corals. FEMS microbiol. Ecol. 18: 155–162.

    Google Scholar 

  • Clarke, R., 1996. Population shifts in two competing fish species on a degrading coral reef. Mar. Ecol. Prog. Ser. 137: 51–58.

    Google Scholar 

  • Colwell, R. R., 1996. Global climate and infectious disease: the cholera paradigm. Science 274: 2025–2031.

    Google Scholar 

  • Cuppers, H., S. Oomes & S. Brul, 1997. A model for the combined effects of temperature and salt concentration on the growth rate of food spoilage. Appl. envir. Microbiol. 63: 3764–3769.

    Google Scholar 

  • Dixon, D. M. & T. J. Walsh, 1992. Human pathogenesis. In Bennett, J. & M. Klich (eds), Aspergillus: Biology and Industrial Applications. Butterworth-Heinemann, Boston: 249–267.

    Google Scholar 

  • Espinel-Ingroff, A., M. Bartlett, R. Bowden, N. Chin, C. Cooper, A. Fothergill, M. McGinnis, P. Menezes, S. Messer, P. Nelson, F. Odds, L. Pasarell, J. Peter, M. Ofaller, J. Rex, M. Rinaldi, G. Shankland, T. Walsh & I. Weitzman, 1997. Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi. J. clin. Microbiol. Jan. 1997: 139–143.

    Google Scholar 

  • Feingold, J. S., 1988. Ecological studies of a cyanobacterial infection of the Caribbean sea plume Pseudopterogorgia acerosa (Coelenterata: Octocorallia). Int. coral reef Sym. 3: 157–162.

    Google Scholar 

  • Ford, S. E., 1996. Range extension by the oyster parasite Perkinsus marinus into the northeastern United States: response to climate change? J. shell. Res. 15: 45–56.

    Google Scholar 

  • Geiser, D., J. Taylor, K. Ritchie & G. Smith, 1998. Cause of sea fan death in the West Indies. Nature 394: 137–138.

    Google Scholar 

  • Gharelb, M. & M. Nour El Dein, 1990. Growth and enzyme activities of Aspergillus sydowii grown on alkali pretreated sawdust as influenced by some culture conditions. Egypt. J. Bot. 33: 191–200.

    Google Scholar 

  • Gladfelter, W. B., 1982. White-band disease in Acropora palmata: implications for the structure and growth of shallow reefs. Bull. mar. Sci. 32: 639–643.

    Google Scholar 

  • Goreau, T. J., J. Cervino, M. Goreau, R. Hayes, M. Hayes, L. Richardson, G. Smith, K. DeMeyer, I. Nagelkerken, J. Garzon-Ferrera, D. Gil, G. Garrison, E. H. Williams, L. Bunkley-Williams, G. Quirolo, K. Patterson, J. W. Porter, & K. Porter, 1998. Rapid spread of diseases in Caribbean coral reefs. Rev. biol. trop. 46Suppl. 5: 157–171.

    Google Scholar 

  • Grigg, R. W. & S. J. Dollar, 1990. Natural and anthropogenic disturbance on coral reefs. In Dubinsky, Z. (ed.), Coral Reefs. Elsevier Science Publishers, Amsterdam: 439–452.

    Google Scholar 

  • Harvell, C.D., K. Kim, J. M. Burkholder, R. R. Colwell, P. R. Epstein, D. J. Grimes, E. E. Hofmann, E. K. Lipp, A. D. M. E. Osterhaus, R. M. Overstreet, J. W. Porter, G. W. Smith, & G. R. Vasta, 1999. Emerging marine diseases–climate links and anthropogenic factors. Science 285: 1505–1510.

    Google Scholar 

  • Holmquist, G. U., H. W. Walker & H. M. Stahr, 1983. Influence of temperature, pH, water activity and antifungal agents on growth of Aspergillus flavus and A. parasiticus. J. food Sci. 8: 778–782.

    Google Scholar 

  • Hughes, T. P., 1994. Catastrophes, phase shifts, and large-scale degradation of a Caribbean coral reef. Science 265: 1547–1551.

    Google Scholar 

  • Kendrick, B., M. Risk, J. Michaelides & K. Bergman, 1982. Amphibious microborers: bioeroding fungi isolated from live corals. Bull. mar. Sci. 32: 862–867.

    Google Scholar 

  • Kim, K., C. D. Harvell, P. D. Kim, G. W. Smith & S. M. Merkel, 2000. Fungal disease resistance of Caribbean sea fan corals (Gorgonia spp.). Mar. Biol. 136: 259–267. 111

    Google Scholar 

  • Kushmaro, A., Y. Loya, M. Fine & E. Rosenberg, 1997. Bleaching of the coral Oculina patagonica by Vibrio AK-1. Mar. Ecol. Prog. Ser. 147: 159–165.

    Google Scholar 

  • Kushmaro, A., Y. Loya, M. Fine & E. Rosenberg, 1996. Bacterial infection and coral bleaching. Nature 380: 396.

    Google Scholar 

  • Kushmaro, A., E. Rosenberg, M. Fine, Y. Ben Haim & Y. Loya, 1998. Effect of temperature on bleaching of the coral Oculina patagonica by Vibrio AK-1. Mar. Ecol. Prog. Ser. 171: 131–137.

    Google Scholar 

  • Kuta, K. G. & L. L. Richardson, 1996. Abundance and distribution of black band disease on corals reefs in the northern Florida Keys. Coral Reefs 15: 219–223.

    Google Scholar 

  • Lessios, H. A., 1988. Mass mortality of Diadema antillarum in the Caribbean: what have we learned? Ann. Rev. Ecol. Syst. 19: 371–393.

    Google Scholar 

  • Lenihan, H., F. Micheli, S. Shelton & C. Peterson, 1999. The in-fluence of multiple environmental stressors on susceptibility to parasites: an experimental determination with oysters. Limnol. Oceanogr. 44: 910–924.

    Google Scholar 

  • Nagelkerken, I., K. Buchan, G. W. Smith, K. Bonair, P. Bush, J. Garzón-Ferreira, L. Boltero, P. Gayle, C. Heberer, C. Petrovic, L. Pors & P. Yoshioka, 1996. Widespread disease in Caribbean sea fans: I. Spreading and general characteristics. Int. coral reef Symp. 1: 679–682.

    Google Scholar 

  • Nagelkerken, I., K. Buchan, G. W. Smith, K. Bonair, P. Bush, J. Garzón-Ferreira, L. Botero, P. Gayle, C. D. Harvell, C. Heberer, K. Kim, C. Petrovic, L. Pors & P. Yoshioka, 1997. Widespread disease in Caribbean sea fans: II. Patterns of infection and tissue loss. Mar. Ecol. Prog. Ser. 160: 255–263.

    Google Scholar 

  • Olutiola, P. O. & O. O. Cole, 1977. Some environmental and nutritional factors affecting growth and sporulation of Aspergillus sydowi. Physiol. Plant 39: 239–242.

    Google Scholar 

  • Pastorok, R. & G. Bilyard, 1985. Effects of sewage pollution on coral-reef communities. Mar. Ecol. Prog. Ser. 21: 175–189.

    Google Scholar 

  • Pontón, J., G. Quindos & R. Cisterna, 1991. Humoral responses of the host to fungi. In Arora, D. K., L. Ajello & K. Mukerji (eds), Handbook of Applied Mycology, Vol. 2. Humans, Animals and Insects. Marcel Dekker, Inc., New York, New York: 289–340.

    Google Scholar 

  • Raghukumar, C. & S. Raghukumar, 1998. Barotolerance of fungi isolated from deep-sea sediments of the Indian Ocean. Aquat. microbiol. Ecol. 15: 153–163.

    Google Scholar 

  • Richardson, L. L., 1998. Coral diseases: what is really known? Trends Ecol. Evol. 13: 438–443.

    Google Scholar 

  • Rinaldi, M. G., 1983. Invasive aspergillosis. Rev. infect. Dis. 5: 1061–1077.

    Google Scholar 

  • Ross, P. S., R. L. De Swart, H. Van Loveren, A. D. M. E. Osterhaus & J. G. Vost, 1996. The immunotoxicity of environmental contaminants to marine wildlife: a review. Annu. Rev. fish Dis. 6: 151–165.

    Google Scholar 

  • Roth, F. J., P. A. Orpurt & D. G. Ahearn, 1964. Occurrence and distribution of fungi in a subtropical marine environment. Can. J. Bot. 42: 375–383.

    Google Scholar 

  • Rützler, K., D. L. Santavy & A. Antonius, 1983. The black band disease of Atlantic reef corals 3. Distribution ecology and development. Mar. Ecol. P. Z. N. I. 4: 329–358.

    Google Scholar 

  • Smith, G. W., C. D Harvell & K. Kim, 1998. Response of sea fans to infection with Aspergillus sp. (Fungi). Rev. biol. trop. 46Supl. 5: 205–208.

    Google Scholar 

  • Smith, G. W., L. D. Ives, I. A. Nagelkerken & K. B. Richie, 1996. Caribbean sea-fan mortalities. Nature 383: 487.

    Google Scholar 

  • Smith, J. M. B., 1989. Aspergillosis. In Opportunistic Mycoses of Man and Other Animals. BPCC Wheatons Ltd, Exeter, Wallingford, U.K.: 81–114.

    Google Scholar 

  • Sweeney, J. C., G. Migaki, P. M. Vainik & R. H. Conklin, 1976. Systemic mycoses in marine mammals. J. am. vet. med. assoc. 169: 946–948.

    Google Scholar 

  • Thompson, J. N., 1998. Rapid evolution as an ecological process. Trends. Ecol. Evol. 13: 329–332.

    Google Scholar 

  • Toren, A., L. Landau, A. Kushmaro, Y. Loya & E. Rosenberg, 1998. Effect of temperature on adhesion of Vibrio strain AK-1 to Oculina patagonica and on coral bleaching. Appl. envir. Microbiol. 64: 1379–1384.

    Google Scholar 

  • Toske, S. G., P. R. Jensen, C. A. Kauffman & W. Fenical, 1998. Aspergillamides A and B: modified cytotoxic tripeptides produced by a marine fungus of the genus Aspergillus. Tetrahed. 54: 13459–13466.

    Google Scholar 

  • Williams, E. H. & L. Bunkley-Williams, 1990. The world-wide coral reef bleaching cycle and related sources of coral mortality. Atoll res. Bull. 335: 1–63.

    Google Scholar 

  • Wilkinson, C., O. Linden, H. Cesar, G. Hodgson, J. Rubens & A. E. Strong, 1999. Ecological and socioeconomic impacts of 1998 coral mortality in the Indian Ocean: An ENSO impact and a warning of future change? Ambio 28:188–196.

    Google Scholar 

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Author notes

  1. Kiho Kim

    Present address: Department of Biology, American University, 4400 Massachusetts Ave., NW Washington, DC, 20016-8007, U.S.A.

Authors and Affiliations

  1. Ecology & Evolutionary Biology, Cornell University, Corson Hall, Ithaca, NY, 14853, U.S.A.

    Alisa P. Alker & Kiho Kim

  2. Department of Biology and Geology, University of South Carolina, Aiken, SC, 29801, U.S.A.

    Garriet W. Smith

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  1. Alisa P. Alker
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Alker, A.P., Smith, G.W. & Kim, K. Characterization of Aspergillus sydowii (Thom et Church), a fungal pathogen of Caribbean sea fan corals. Hydrobiologia 460, 105–111 (2001). https://doi.org/10.1023/A:1013145524136

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