Abstract
Several plant species are infected by different species of the genus Agrobacterium. One problem is that no rapid and sensitive method is available for the identification of isolates of Agrobacterium at the species level. The usefulness of LMW RNA profiles for the identification of Agrobacterium species was examined. The profiles of strains belonging to the proposed species were identical to those of the type strain of each species except in two cases. In A. radiobacter, two groups of strains with different tRNA profiles were detected and in A. rhizogenes two groups with different 5S rRNA zones were found. Nevertheless, with the LMW RNA profiles it was possible to assign any isolate to one of each group within these species. The results obtained showed that all isolates studied here can be assigned to a species of Agrobacterium and hence that LMW RNA profiles offer a suitable method for the identification of tumor-inducing bacteria.
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References
Bergey's Manual of Determinative Bacteriology (1994). Williams and Wilkins, Baltimore
Bergersen FJ (1961) The growth of Rhizobium in synthetic media. Australian Journal of Biology 14: 349–360
Bidle KD and Fletcher M (1995) Comparison of free-living and particle-associated bacterial communities in the Chesapeake Bay by stable low-molecular-weight RNA analysis. Applied Environmental Microbiology 61: 944–952
Bouzar H, Chilton WS, Nesme X, Dessaux Y, Vaudequin V, Petit A, Jones JB and Hodge NC (1995) A new Agrobacterium strain isolated from aerial tumours on Ficus benjamina L. Applied Environmental Microbiology 61: 65–73
Bouzar H and Jones JB (2001) Agrobacterium larrymoorei sp. nov. a pathogen isolated from aerial tumours of Ficus benjamina. International Journal of Systematic and Evolutionary Microbiology 51: 1023–1026
Cruz-Sánchez JM, Velázquez E, Mateos P and Martínez-Molina E (1997) Enhancement of resolution of low molecular weight RNA profiles by staircase electrophoresis. Electrophoresis 18: 1909–1911
de Lajudie P, Laurent-Fullele E, Willems A, Torck U, Coopman R, Collins CD, Kersters K, Dreyfus B and Gillis M (1998) Allorhizobium undicola gen. nov., sp. nov., nitrogen-fixing bacteria that efficiently nodulate Neptunia natans in Senegal. International Journal of Systematic Bacteriology 48: 1277–1290
de Lajudie P, Willems A, Nick G, Mohamed SH, Torck U, Coopman R, Filali-Maltouf A, Kersters, K, Dreyfus B, Lindström K and Gillis M (1999) Agrobacterium bv. 1 strains isolated from nodules of tropical legumes. Systematic and Applied Microbiology 22: 119–132
Haas H, Budowle B and Weiler G (1994) Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments. Electrophoresis 15: 153–158
Höfle MG (1988) Identification of bacteria by low molecular weight RNA profiles: a new chemotaxonomic approach. Journal of Microbiological Methods 8: 235–248
Kersters K and de Ley J (1984) Genus III. Agrobacterium. In: Krieg NR and Holt JG (eds) Bergey's Manual of Systematic Bacteriology, Vol 1 (pp 244–254) Williams and Wilkins Co, Baltimore
Ophel K and Kerr A (1990) Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines. International Journal of Systematic Bacteriology 40: 236–241
Palomo JL, Velázquez E, Mateos PF, García-Benavides P and Martínez-Molina E (2000) Rapid identification of Clavibacter michiganensis subspecies sepedonicus based on the stable low molecular weight RNA (LMW RNA) profiles. European Journal of Plant Pathology 106: 789–793
Paulawska J, Maes M, Willems A and Sobiczewski P (2000) Phylogenetic analysis of 23S rRNA gene sequences of Agrobacterium, Rhizobium and Sinorhizobium strains. Systematic and Applied Microbiology 23: 238–244
Plazinski J, Chen YH and Rolfe BG (1985) General method for the identification of plasmid species in fast-growing soil microorganisms. Applied and Environmental Microbiology 48: 1001–1003
Rüger HJ and Höfle M (1992) Marine star-shaped-aggregateforming bacteria: Agrobacterium atlanticum sp. nov., Agrobacterium meteori sp. nov., Agrobacterium ferrugineum sp. nov., nom. rev., Agrobacterium gelatinovorum sp. nov. nom. rev.; and A. stellulatum sp. nov. nom. rev. International Journal of Systematic Bacteriology 42: 133–143
Ridé M, Ridé S, Petit A, Bollet C, Dessaux Y and Gardan L (2000) Characterization of plasmid-borne and chromosomeencoded traits of Agrobacterium biovar 1, 2 and 3 strains from France. Applied and Environmental Microbiology 66: 1818–1825
Sawada H, Ieki H, Oyaizu H and Matsumoto S (1993) Proposal for rejection of Agrobacterium tumefaciens and revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes. International Journal of Systematic and Evolutionary Microbiology 43: 694–702
Sprinzl M, Moll J, Meissner F and Hatmann T(1985) Compilation of tRNA sequences. Nucleic Acid Research 13: 1–49
Toro N and Olivares J (1986) Characterization of a large plasmid of Rhizobium meliloti involved in enhancing nodulation. Molecular General Genetics 202: 331–335
Velázquez E, Cervantes E, Igual JM, Peix A, Mateos PF, Benamar S, Moiroud A, Wheeler CT, Dawson JO, Labeda D, Rodríguez-Barrueco C and Martínez-Molina E (1998a) Analysis of LMW RNA profiles of Frankia strains by staircase electrophoresis. Systematic and Applied Microbiology 21: 539–545
Velázquez E, Cruz-Sánchez JM, Mateos PF and Martínez-Molina E (1998b) Analysis of stable Low Molecular Weight RNA Profiles of members of the family Rhizobiaceae. Applied and Environmental Microbiology 64: 1555–1559
Velázquez E, Calvo O, Cervantes E, Mateos PF, Tamame M and Martínez-Molina E (2000a) Staircase electrophoresis profiles of stable low-molecular-weight RNA — a new technique for yeast fingerprinting. International Journal of Systematic and Evolutionary Microbiology 50: 917–923
Velázquez E, Igual JM, Willems A, Fernández MP, Muñoz E, Mateos PF, Abril A, Toro N, Normand P, Cervantes E, Gillis M and Martínez-Molina E (2001a) Description of Mesorhizobium chacoense sp. nov. that nodulates prosopis alba in the Chaco Arido region (Argentina). International Journal of Systematic and Evolutionary Microbiology 51: 1011–1021
Velázquez E, Martínez-Romero E, Rodríguez-Navarro DN, Trujillo ME, Daza A, Mateos PF, Martínez-Molina E and van Berkum P (2001b) Characterization of rhizobial isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low Molecular Weight RNA. Applied and Environmental Microbiology 67: 1008–1010
Young JM, Kuykendall LD, Martínez-Romero E, Kerr A and Sawada H (2001) A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al., 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. International Journal of Systematic and Evolutionary Microbiology 51: 89–103
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Velázquez, E., Luis Palomo, J., Lastra, B. et al. Rapid Identification of Agrobacterium Species By Staircase Electrophoresis of Low Molecular Weight RNA Profiles. European Journal of Plant Pathology 107, 931–938 (2001). https://doi.org/10.1023/A:1013140709873
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DOI: https://doi.org/10.1023/A:1013140709873