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Genotyping of Anatolian doubled-haploid durum lines with SSR markers

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Abstract

In this study, doubled haploid lines generated from the durum wheat varieties, selections from Middle Anatolian landraces, ‘Çakmak-79’, ‘Berkmen-469’ and ‘Kunduru-1149’ (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The average PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars ‘Çakmak-79’ and ‘Kunduru-1149’, were the two most distant populations with (δ μ)2 = 1.42. ‘Berkmen-469’ x ‘Çakmak-79’ and ‘Berkmen-469’ x ‘Kunduru-1149’ yielded similar genetic distances, (δμ)2 of 0.84 and 0.85, respectively. In addition, the genetic relationship between the progenitors of the DH lines together with other durum wheat varieties was analyzed. A meaningful relationship was obtained based on available pedigree information on the cultivars.

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Authors and Affiliations

  1. Department of Chemistry, Biochemistry Division, Middle East Technical University, Ankara, TR-06531

    Erdogan E. Hakki

  2. Nuclear Agriculture and Animal Research Center, Saray, Ankara, TR-06983, Turkey

    Cigdem Savaskan

  3. Department of Chemistry, Biochemistry Division, Middle East Technical University, Ankara, TR-06531, Turkey

    Mahinur S. Akkaya

Authors
  1. Erdogan E. Hakki
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  2. Cigdem Savaskan
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  3. Mahinur S. Akkaya
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Correspondence to Mahinur S. Akkaya.

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Hakki, E.E., Savaskan, C. & Akkaya, M.S. Genotyping of Anatolian doubled-haploid durum lines with SSR markers. Euphytica 122, 257–262 (2001). https://doi.org/10.1023/A:1012955516198

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  • DOI: https://doi.org/10.1023/A:1012955516198

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