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Zinc metabolism and homeostasis: The application of tracer techniques to human zinc physiology

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Abstract

Tracer kinetic techniques based on zinc stable isotopes have a vital role in advancing knowledge of human zinc physiology and homeostasis. These techniques have demonstrated the complexity of zinc metabolism, and have been critical to estimating the size and interrelationships of those pools of zinc that exchange rapidly with zinc in plasma and which are likely to be especially important for zinc dependent biology. This paper presents findings from recent research linking a steady state compartmental model with non-steady state post-prandial sampling from the intestine, utilizing a combination of intestinal intubation/perfusion and stable isotope tracer kinetic techniques. The gastrointestinal tract has a central role in maintaining whole body zinc homeostasis. While the fractional absorption of zinc from a meal depends on the quantity of exogenous zinc and on such dietary factors as phytic acid, the fractional absorption does not appear to be dependent on the size of the rapidly exchanging pool of the host. In contrast, the quantity of endogenous zinc excreted via the intestine is positively correlated with both the amount of absorbed zinc and the zinc `status' of the host, and thus this process has an equally critical role in maintaining zinc homeostasis. The observed alterations in zinc metabolism in some disease states can be understood in the context of known homeostatic processes. In other conditions, however, such alterations as inflammation-associated hyperzincuria and zinc redistribution, the links between homeostatic perturbation and cellular biology are yet to be explained. Thus the challenge remains for research at the whole body level to carefully characterize zinc distribution and exchange under diverse circumstances, while research at the cellular level must elucidate the regulatory processes and the factors to which they respond.

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References

  • Abou-Mohamed G, Papapetropoulos A, Catravas JD, Caldwell RW. 1998 Zn2+ inhibits nitric oxide formation in response to lipopolysaccharides: implication in its anti-inflammatory activity. Eur J Pharmacol 341, 265-272.

    Google Scholar 

  • Adams CL, Hambridge KM, Raboy V, Dorsch JA, Sian L, Westcott JE, Krebs NF. 2001 Zinc absorption from a low phytic acid maize. Am J Clin Nutr, in press.

  • Adler M, Robberecht P, Mestdagh M, Cremer M, Delcourt A, Christophe J. 1980 The pancreatic secretion of zinc in man and rat. Gastroenterol Clin Biol 4, 441-449.

    Google Scholar 

  • Agren MS. 1990 Studies on zinc in wound healing. Acta Derm Venereol Suppl 154, 1-36.

    Google Scholar 

  • Andrews GK, Kage K, Palmiter-Thomas P, Sarras MP, Jr. 1990 Metal ions induce expression of metallothionein in pancreatic exocrine and endocrine cells. Pancreas 5, 548-554.

    Google Scholar 

  • Antonucci A, Di Baldassarre A, Di Giacomo F, Stuppia L, Palka G. 1997 Detection of apoptosis in peripheral blood cells of 31 subjects affected by Down syndrome before and after zinc therapy. Ultrastruct Pathol 21, 449-452.

    Google Scholar 

  • Askari A, Long CL, Blakemore WS. 1982 Net metabolic changes of zinc, copper, nitrogen, and potassium balances in skeletal trauma patients. Metabolism 31, 1185-1193.

    Google Scholar 

  • Beisel WR. 1995 Herman Award Lecture, 1995: Infection-induced malnutrition-from cholera to cytokines. Am J Clin Nutr 62, 813-819.

    Google Scholar 

  • Bhutta ZA, Black RE, Brown KH, Gardner JM, Gore S, Hidayat A, Khatun F, Martorell R, Ninh NX, Penny ME, Rosado JL, Roy SK, Ruel M, Sazawal S, Shankar A. 1999 Prevention of diarrhea and pneumonia by zinc supplementation in children in developing countries: pooled analysis of randomized controlled trials. Zinc Investigators’ Collaborative Group. J Pediatr 135, 689-697.

    Google Scholar 

  • Birnstingle M, Stone B, Richards V. 1956 Excretion of radioactive zinc (65Zn) in bile, pancratic and duodenal secretions in the dog. Am J Physiol 186, 377-379.

    Google Scholar 

  • Blanchard RK, Cousins RJ. 1997 Upregulation of rat intestinal uroguanylin mRNA by dietary zinc restriction. Am J Physiol 272, G972-G978.

    Google Scholar 

  • Bremner I, Beattie JH. 1990 Metallothionein and the trace minerals. Annu Rev Nutr 10, 63-83.

    Google Scholar 

  • Brown KH, Peerson JM, Allen LH. 1998 Effect of zinc supplementation on children's growth: a meta-analysis of intervention trials. Bibl Nutr Dieta 54, 76-83.

    Google Scholar 

  • Caulfield LE, Zavaleta N, Figueroa A. 1999 Adding zinc to prenatal iron and folate supplements improves maternal and neonatal zinc status in a Peruvian population. Am J Clin Nutr 69, 1257-1263.

    Google Scholar 

  • Caulfield LE, Zavaleta N, Shankar AH, Merialdi M. 1998 Potential contribution of maternal zinc supplementation during pregnancy to maternal and child survival. Am J Clin Nutr 68, 499S-508S.

    Google Scholar 

  • Chausmer AB. 1998 Zinc, insulin and diabetes. J Am Coll Nutr 17, 109-115.

    Google Scholar 

  • Chesters JK. 1982 Metabolism and biochemistry of zinc. In: Prasad AS. ed. Clinical, Biochemical, and Nutritional Aspects of Trace Elements. Vol. 6. Current Topics in Nutrition and Disease. New York: Alan R. Liss; 221-238.

    Google Scholar 

  • Chiricolo M, Musa AR, Monti D, Zannotti M, Franceschi C. 1993 Enhanced DNA repair in lymphocytes of Down syndrome patients: the influence of zinc nutritional supplementation. Mutat Res 295, 105-111.

    Google Scholar 

  • Cousins RJ. 1998 A role of zinc in the regulation of gene expression. Proc Nutr Soc 57, 307-311.

    Google Scholar 

  • Cousins RJ, Dunn MA, Leinart AS, Yedinak KC, DiSilvestro RA 1986 Coordinate regulation of zinc metabolism and metallothionein gene expression in rats. Am J Physiol 251, E688-E694.

    Google Scholar 

  • Dalton T, Fu K, Palmiter RD, Andrews GK. 1996 Transgenic mice that overexpress metallothionein-I resist dietary zinc deficiency. J Nutr 126, 825-833.

    Google Scholar 

  • Davies NT, Williams RB. 1977 The effect of pregnancy and lactation on the absorption of zinc and lysine by the rat duodenum in situ. Br J Nutr 38, 417-423.

    Google Scholar 

  • De Lisle RC, Sarras MP, Jr., Hidalgo J, Andrews GK. 1996 Metallothionein is a component of exocrine pancreas secretion: implications for zinc homeostasis. Am J Physiol 271, C1103-C1110.

    Google Scholar 

  • Dijkstra M, Kuipers F, Smit EP, de Vries JJ, Havinga R, Vonk, RJ. 1991 Biliary secretion of trace elements and minerals in the rat. Effects of bile flow variation and diurnal rhythms. J Hepatol 13, 112-119.

    Google Scholar 

  • Dunn MA, Cousins RJ. 1989 Kinetics of zinc metabolism in the rat: effect of dibutyryl cAMP. Am J Physiol 256, E420-E430.

    Google Scholar 

  • Easley D, Krebs N, Jefferson M, Miller L, Erskine J, Accurso F, Hambidge KM. 1998 Effect of pancreatic enzymes on zinc absorption in cystic fibrosis. J Pediatr Gastroenterol Nutr 26, 136-139.

    Google Scholar 

  • Ehrenkranz RA, Gettner PA, Nelli CM, Sherwonit EA, Williams JE, Ting BT, Janghorbani M. 1989 Zinc and copper nutritional studies in very low birth weight infants: comparison of stable isotopic extrinsic tag and chemical balance methods. Pediatr Res 26, 298-307.

    Google Scholar 

  • Finley JW, Johnson PE, Reeves PG, Vanderpool RA, Briske-Anderson M. 1994 Effect of bile/pancreatic secretions on absorption of radioactive or stable zinc. In vivo and in vitro studies. Biol Trace Elem Res 42, 81-96.

    Google Scholar 

  • Fleming CR, Huizenga KA, McCall JT, Gildea J, Dennis R 1981 Zinc nutrition in Crohn's disease. Dig Dis Sci 26, 865-870.

    Google Scholar 

  • Food and Nutrition Board, Institute of Medicine 2001 (pre-print). Dietary Reference Intakes for Vitamin A, Vitamin K, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium and Zinc. Washington, DC: National Academy Press.

    Google Scholar 

  • Fraker PJ, King LE, Laakko T, Vollmer TL. 2000 The dynamic link between the integrity of the immune system and zinc status. J Nutr 130, 1399S-1406S.

    Google Scholar 

  • Frederickson CJ, Suh SW, Silva D, Thompson RB. 2000 Importance of zinc in the central nervous system: the zinc-containing neuron. J Nutr 130, 1471S-1483S.

    Google Scholar 

  • Friel JK, Andrews WL, Simmons BS, Miller LV, Longerich HP 1996 Zinc absorption in premature infants: comparison of two isotopic methods. Am J Clin Nutr 63, 342-347.

    Google Scholar 

  • Fung EB, Ritchie LD, Woodhouse LR, Roehl R, King JC. 1997 Zinc absorption in women during pregnancy and lactation: a longitudinal study. Am J Clin Nutr 66, 80-88.

    Google Scholar 

  • Gabay C, Kushner I. 1999 Acute-phase proteins and other systemic responses to inflammation. N Engl J Med 340, 448-454.

    Google Scholar 

  • Gibson RS. 1994 Zinc nutrition in developing countries. Nutr Res Rev 7, 151-173.

    Google Scholar 

  • Goldenberg RL, Tamura T, Neggers Y, Copper RL, Johnston KE, DuBard MB, Hauth JC. 1995 The effect of zinc supplementation on pregnancy outcome. JAMA 274, 463-468.

    Google Scholar 

  • Hambidge KM, Hambidge C, Jacobs M, Baum JD. 1972 Low levels of zinc in hair, anorexia, poor growth, and hypogeusia in children. Pediatr Res 6, 868-874.

    Google Scholar 

  • Hambidge KM, Krebs NF, Lilly JR, Zerbe GO. 1987 Plasma and urine zinc in infants and children with extrahepatic biliary atresia. J Pediatr Gastroenterol Nutr 6, 872-877.

    Google Scholar 

  • Hambidge KM, Krebs NF, Miller L. 1998 Evaluation of zinc metabolism with use of stable-isotope techniques: implications for the assessment of zinc status. Am J Clin Nutr 68, 410S-413S.

    Google Scholar 

  • Hambidge M, Krebs NF. 2001. Interrelationships of key variables of human zinc homeostasis: relevance to dietary zinc requirements. Annu Rev Nutr 21, 429-452.

    Google Scholar 

  • Hernandez J, Giralt M, Belloso E, Rebollo DV, Romero B, Hidalgo J. 1996. Interactions between metallothionein inducers in rat liver and primary cultures of rat hepatocytes. Chem Biol Interact 100, 27-40.

    Google Scholar 

  • House WA, Wastney ME. 1997 Compartmental analysis of zinc kinetics in mature male rats. Am J Physiol 273, R1117-R1125.

    Google Scholar 

  • Hunt JR, Mullen LK, Lykken GI. 1992 Zinc retention from an experimental diet based on the US FDA Total Diet Study. Nutr Res 127, 1335-1344.

    Google Scholar 

  • Iwata M, Takebayashi T, Ohta H, Alcalde RE, Itano Y, Matsumura T. 1999 Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skin. Histochem Cell Biol 112, 283-290.

    Google Scholar 

  • Jackson MJ, Giugliano R, Giugliano LG, Oliveira EF, Shrimpton R, Swainbank IG. 1988 Stable isotope metabolic studies of zinc nutrition in slum-dwelling lactating women in the Amazon valley. Br J Nutr 59, 193-203.

    Google Scholar 

  • Jackson MJ, Jones DA, Edwards RH, Swainbank IG, Coleman ML 1984 Zinc homeostasis in man: studies using a new stable isotope-dilution technique. Br J Nutr 51, 199-208.

    Google Scholar 

  • Jalla S, Krebs NF, Rodden IDJ, Miller LV. 1997 Zinc homeostasis in very low birth weight infants-a comparison of human milk and formulas. Pediat Res 41, 233A.

    Google Scholar 

  • Johnson PE, Hunt CD, Milne DB, Mullen LK. 1993 Homeostatic control of zinc metabolism in men: zinc excretion and balance in men fed diets low in zinc. Am J Clin Nutr 57, 557-565.

    Google Scholar 

  • Kelly EJ, Quaife CJ, Froelick GJ, Palmiter RD. 1996 Metallothionein I and II protect against zinc deficiency and zinc toxicity in mice. J Nutr 126, 1782-1790.

    Google Scholar 

  • King J. 2000 Determinants of maternal zinc status during pregnancy. Am J Clin Nutr 71 (suppl), 1334S-1343S.

    Google Scholar 

  • King J, Turnlund J. 1989 Human Zinc Requirements. In: Mills C. ed. Zinc in Human Biology. London: Human Nutrition Reviews. Springer-Verlag; 335-350.

    Google Scholar 

  • King JC, Shames DM, Woodhouse LR. 2000 Zinc homeostasis in humans. J Nutr 130, 1360S-1366S.

    Google Scholar 

  • Krebs N, Westcott J, Miller L, Herrmann T, Hambidge K. 2000a Exchangeable zinc pool (EZP) in normal infants: correlates with parameters of zinc homeostasis. FASEB J 14, A205.

    Google Scholar 

  • Krebs NF, Hambidge KM. 1986 Zinc requirements and zinc intakes of breast-fed infants. Am J Clin Nutr 43, 288-292.

    Google Scholar 

  • Krebs NF, Reidinger CJ, Miller LV, Hambidge KM. 1996 Zinc homeostasis in breast-fed infants. Pediatr Res 39, 661-665.

    Google Scholar 

  • Krebs NF, Sontag M, Accurso FJ, Hambidge KM. 1998a Low plasma zinc concentrations in young infants with cystic fibrosis. J Pediatr 133, 761-764.

    Google Scholar 

  • Krebs NF, Westcott J. 2001 Zinc and breastfed infants: If and when is there a risk of deficiency? In: Proceedings of 10th International Conference, International Society for Research in Human Milk and Lactation, Vol. in press. Plenum Press.

  • Krebs NF, Westcott J, Miller LV. 1999 Localization of secretion and reabsorption of endogenous zinc by compartmental modeling of intestinal data. FASEB J 13, A214.

    Google Scholar 

  • Krebs NF, Westcott JE, Arnold TD, Kluger BM, Accurso FJ, Miller LV, Hambidge KM. 2000b Abnormalities in zinc homeostasis in young infants with cystic fibrosis. Pediatr Res 48, 256-261.

    Google Scholar 

  • Krebs NF, Westcott JE, Huffer JW, Miller LV. 1998b Absorption of exogenous zinc (Zn) and secretion of endogenous Zn in the human small intestine. FASEB J 12, A345.

    Google Scholar 

  • Krebs NF, Westcott JE, Sian L, Miller LV. 2001 Effect of dietary zinc (Zn) intake restriction on net secretion of intestinal endogenous zinc. FASEB J 14, A402.

    Google Scholar 

  • Lee DY, Prasad AS, Hydrick-Adair C, Brewer G, Johnson PE 1993 Homeostasis of zinc in marginal human zinc deficiency: role of absorption and endogenous excretion of zinc. J Lab Clin Med 122, 549-556.

    Google Scholar 

  • Lee HH, Hill GM, Sikha VK, Brewer GJ, Prasad AS, Owyang C 1990 Pancreaticobiliary secretion of zinc and copper in normal persons and patients with Wilson's disease. J Lab Clin Med 116, 283-288.

    Google Scholar 

  • Lehman-McKeeman LD, Andrews GK, Klaassen CD. 1988 Induction of hepatic metallothioneins determined at isoprotein and messenger RNA levels in glucocorticoid-treated rats. Biochem J 249, 429-433.

    Google Scholar 

  • Lei S, Hambidge KM, Westcott JL, Miller LV, Fennessey PV 1993 Influence of a meal and incremental doses of zinc on changes in zinc absorption. Am J Clin Nutr 58, 533-536.

    Google Scholar 

  • Lei S, Krebs NF, Westcott JE, Miller LV, Hambidge KM. 2001 Zinc homeostasis during lactation in a population with low zinc intakes. Am J Clin Nutr, in press.

  • Lei S, Mingyan X, Miller LV, Tong L, Krebs NF, Hambidge KM 1996 Zinc absorption and intestinal losses of endogenous zinc in young Chinese women with marginal zinc intakes. Am J Clin Nutr 63, 348-353.

    Google Scholar 

  • Licastro F, Chiricolo M, Mocchegiani E, Fabris N, Zannoti M, Beltrandi E, Mancini R, Parente R, Arena G, Masi M. 1994a Oral zinc supplementation in Down's syndrome subjects decreased infections and normalized some humoral and cellular immune parameters. J Intellect Disabil Res 38, 149-162.

    Google Scholar 

  • Licastro F, Morini MC, Davis LJ. 1994b Neuroendocrine immune modulation induced by zinc in a progeroid disease-Down's syndrome. Ann N Y Acad Sci 717, 299-306.

    Google Scholar 

  • Lowe NM, Bremner I, Jackson MJ. 1991 Plasma 65Zn kinetics in the rat. Br J Nutr 65, 445-455.

    Google Scholar 

  • Lowe NM, Hall EJ, Anderson RS, Batt RM, Jackson MJ. 1995 A stable isotope study of zinc kinetics in Irish setters with glutensensitive enteropathy. Br J Nutr 74, 69-76.

    Google Scholar 

  • Lowe NM, Jackson MJ. 2000 Kinetic studies of whole-body traceelement metabolism. In: Lowe NM, Jackson MJ. eds. Advances in Isotope Methods for the Analysis of Trace Elements in Man. London: CRC Press; 81-91.

    Google Scholar 

  • Lowe NM, Shames DM, Woodhouse LR, Matel JS, Roehl R, Saccomani MP, Toffolo G, Cobelli C, King JC. 1997 A compartmental model of zinc metabolism in healthy women using oral and intravenous stable isotope tracers. Am J Clin Nutr 65, 1810-1819.

    Google Scholar 

  • Manary MJ, Hotz C, Krebs NF, Gibson RS, Westcott JE, Arnold T, Broadhead RL, Hambidge KM. 2000 Dietary phytate reduction improves zinc absorption in Malawian children recovering from tuberculosis but not in well children. J Nutr 130, 2959-2964.

    Google Scholar 

  • Matseshe JW, Phillips SF, Malagelada JR, McCall JT. 1980 Recovery of dietary iron and zinc from the proximal intestine of healthy man: studies of different meals and supplements. Am J Clin Nutr 33, 1946-1953.

    Google Scholar 

  • McClain CJ. 1990 The pancreas and zinc homeostasis [editorial]. J Lab Clin Med 116, 275-276.

    Google Scholar 

  • McCormick CC, Menard MP, Cousins RJ. 1981 Induction of hepatic metallothionein by feeding zinc to rats of depleted zinc status. Am J Physiol 240, E414-E421.

    Google Scholar 

  • McMahon RJ, Cousins RJ. 1998 Mammalian zinc transporters. J Nutr 128, 667-670.

    Google Scholar 

  • Melichar B, Jandik P, Tichy M, Malir F, Mergancova J, Voboril Z. 1994 Urinary zinc excretion and acute phase response in cancer patients. Clin Investig 72, 1012-1014.

    Google Scholar 

  • Miller LV, Hambidge KM, Naake VL, Hong Z, Westcott JL, Fennessey PV. 1994 Size of the zinc pools that exchange rapidly with plasma zinc in humans: alternative techniques for measuring and relation to dietary zinc intake. J Nutr 124, 268-276.

    Google Scholar 

  • Miller LV, Krebs NF, Hambidge KM. 1998 Human zinc metabolism: advances in the modeling of stable isotope data. Adv Exp Med Biol 445, 253-269.

    Google Scholar 

  • Miller LV, Krebs NF, Hambidge KM. 2000 Development of a compartmental model of human zinc metabolism: identifiability and multiple studies analyses. Am J Physiol 279, R1671-R1684.

    Google Scholar 

  • Miller LV, Krebs NF, Jefferson M, Easley D, Hambidge KM 1997 Compartmental modeling of human zinc metabolism: evaluation of a method for estimating the size of the rapidly exchanging pool of zinc. In: Proceedings of Trace Element Metabolism in Man and Animals-9. Ottawa: NRC Research Press; 144-145.

    Google Scholar 

  • Moser-Veillon PB, Patterson KY, Veillon C. 1996 Zinc absorption is enhanced during lactation. FASEB J 10, A729.

    Google Scholar 

  • Napolitano G, Palka G, Grimaldi S, Giuliani C, Laglia G, Calabrese G, Satta MA, Neri G, Monaco F. 1990 Growth delay in Down syndrome and zinc sulphate supplementation. Am J Med Genet Suppl 7, 63-65.

    Google Scholar 

  • Narkewicz MR, Krebs N, Karrer F, Orban-Eller K, Sokol RJ 1999 Correction of hypozincemia following liver transplantation in children is associated with reduced urinary zinc loss. Hepatology 29, 830-833.

    Google Scholar 

  • Onosaka S, Min KS, Fujita Y, Tanaka K, Iguchi S, Okada Y 1988 High concentration of pancreatic metallothionein in normal mice. Toxicology 50, 27-35.

    Google Scholar 

  • Palmiter RD, Findley SD. 1995 Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc. EMBO J 14, 639-649.

    Google Scholar 

  • Prasad A. 1993 Biochemistry of Metallothionein. In: Biochemistry of Zinc Biochemistry of the Elements. New York and London: Plenum Press; 77-92.

    Google Scholar 

  • Prasad AS, Halsted JA, Nadimi M. 1961 Syndrome of iron defi-ciency anemia, hepatosplenomegaly, hypogonadism, dwarfism and geophagia. Am J Med 31, 532-546.

    Google Scholar 

  • Quinones SR, Cousins RJ. 1984 Augmentation of dexamethasone induction of rat liver metallothionein by zinc. Biochem J 219, 959-963.

    Google Scholar 

  • Rink L, Kirchner H. 2000 Zinc-altered immune function and cytokine production. J Nutr 130, 1407S-1411S.

    Google Scholar 

  • Rofe AM, Winters N, Hinskens B, Philcox JC, Coyle P. 1999 The role of the pancreas in intestinal zinc secretion in metallothionein-null mice. Pancreas 19, 69-75.

    Google Scholar 

  • Sandstrom B. 1997 Bioavailability of zinc. Eur J Clin Nutr 51 (Suppl 1), S17-S19.

    Google Scholar 

  • Sandstrom B, Lonnerdal B. 1989 Promotors and Antagonists of Zinc Absorption. In: Mills CF. ed. Zinc in Human Biology. London: ILSI Human Nutrition Reviews. Springer-Verlag; 57-78.

    Google Scholar 

  • Sandstrom B, Sandberg AS. 1992 Inhibitory effects of isolated inositol phosphates on zinc absorption in humans. J Trace Elem Electrolytes Health Dis 6, 99-103.

    Google Scholar 

  • Schroeder JJ, Cousins RJ. 1990 Interleukin 6 regulates metallothionein gene expression and zinc metabolism in hepatocyte monolayer cultures. Proc Natl Acad Sci USA 87, 3137-3141.

    Google Scholar 

  • Scott ME, Koski KG. 2000 Zinc deficiency impairs immune responses against parasitic nematode infections at intestinal and systemic sites. J Nutr 130, 1412S-1420S.

    Google Scholar 

  • Shankar AH, Prasad AS. 1998 Zinc and immune function: the biological basis of altered resistance to infection. Am J Clin Nutr 68, 447S-463S.

    Google Scholar 

  • Stabile A, Pesaresi MA, Stabile AM, Pastore M, Sopo SM, Ricci R, Celestini E, Segni G. 1991 Immunodeficiency and plasma zinc levels in children with Down's syndrome: a long-term follow-up of oral zinc supplementation. Clin Immunol Immunopathol 58, 207-216.

    Google Scholar 

  • Sturniolo GC, Mestriner C, Irato P, Albergoni V, Longo G, D'Inca R. 1999 Zinc therapy increases duodenal concentrations of metallothionein and iron in Wilson's disease patients. Am J Gastroenterol 94, 334-338.

    Google Scholar 

  • Sullivan JF, Lankford HG. 1965 Zinc metabolism and chronic alcoholism. Am J Clin Nutr 17, 57-63.

    Google Scholar 

  • Sullivan JF, O'Grady J, Lankford HG. 1965 The zinc content of pancreatic secretion. Gastroenterology 48, 438-443.

    Google Scholar 

  • Sustrova M, Strbak V. 1994 Thyroid function and plasma immunoglobulins in subjects with Down's syndrome (DS) during ontogenesis and zinc therapy. J Endocrinol Invest 17, 385-390.

    Google Scholar 

  • Taylor CM, Bacon JR, Aggett PJ, Bremner I. 1991 Homeostatic regulation of zinc absorption and endogenous losses in zincdeprived men. Am J Clin Nutr 53, 755-763.

    Google Scholar 

  • Turnlund JR, Durkin N, Costa F, Margen S. 1986 Stable isotope studies of zinc absorption and retention in young and elderly men. J Nutr 116, 1239-1247.

    Google Scholar 

  • Turnlund JR, King JC, Keyes WR, Gong B, Michel MC. 1984 A stable isotope study of zinc absorption in young men: effects of phytate and alpha-cellulose. Am J Clin Nutr 40, 1071-1077.

    Google Scholar 

  • Van Wouwe JP, Uijlenbroek JJ. 1994 The role of the pancreas in the regulation of zinc status. Biol Trace Elem Res 42, 143-149.

    Google Scholar 

  • Wada L, Turnlund JR, King JC. 1985 Zinc utilization in young men fed adequate and low zinc intakes. J Nutr 115, 1345-1354.

    Google Scholar 

  • Walravens PA, Hambidge KM. 1976 Growth of infants fed a zinc supplemented formula. Am J Clin Nutr 29, 1114-1121.

    Google Scholar 

  • Wapnir RA. 2000 Zinc deficiency, malnutrition and the gastrointestinal tract. J Nutr 130, 1388S-1392S.

    Google Scholar 

  • Wastney ME. 1989 Zinc absorption in humans determined using in vivo tracer studies and kinetic analysis. Adv Exp Med Biol 249, 13-25.

    Google Scholar 

  • Wastney ME, Aamodt RL, Rumble WF, Henkin RI. 1986 Kinetic analysis of zinc metabolism and its regulation in normal humans. Am J Physiol 251, R398-R408.

    Google Scholar 

  • Wastney ME, Ahmed S, Henkin RI. 1992 Changes in regulation of human zinc metabolism with age. Am J Physiol 263, R1162-R1168.

    Google Scholar 

  • Wastney ME, Angelus P, Barnes RM, Subramanian KN. 1996 Zinc kinetics in preterm infants: a compartmental model based on stable isotope data. Am J Physiol 271, R1452-R1459.

    Google Scholar 

  • Wastney ME, Angelus PA, Barnes RM, Subramanian KN. 1999 Zinc absorption, distribution, excretion, and retention by healthy preterm infants. Pediatr Res 45, 191-196.

    Google Scholar 

  • Wastney ME, Gokmen IG, Aamodt RL, Rumble WF, Gordon GE, Henkin RI. 1991 Kinetic analysis of zinc metabolism in humans after simultaneous administration of 65Zn and 70Zn. Am J Physiol 260, R134-R141.

    Google Scholar 

  • Weigand E. 1983 Absorption of trace elements: zinc. Int J Vitam Nutr Res Suppl 25, 67-81.

    Google Scholar 

  • WHO. 1996 Trace Elements in Human Nutrition and Health.: Geneva.

  • Williams RJP. 1989 An introduction to the biochemistry of zinc. In: Mills CF. ed. Zinc in Human Biology, Vol. 4. London: Springer-Verlag; 15-31.

    Google Scholar 

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Krebs, N.F., Hambidge, K.M. Zinc metabolism and homeostasis: The application of tracer techniques to human zinc physiology. Biometals 14, 397–412 (2001). https://doi.org/10.1023/A:1012942409274

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