Abstract
Seven populations collected at different altitudes in northern Italy, two Ladino breeding populations and two control cultivars (AberHerald and Grasslands Huia) of white clover (Trifolium repens L.) were included in a series of experiments analysing: (i) levels of cold tolerance using artificial and field-based methods; (ii) relationships between these measures of cold tolerance; (iii) components of spring yield, various physiological traits, and their relationships with cold tolerance. Rates of seedling and growing point mortality in the populations over winter, assessed in separate field experiments, were closely related (r = 0.85). Grasslands Huia showed the highest death rates, and material originating from high altitudes the lowest. The LT50 value, i.e., the temperature at which 50% of the growing points would die, estimated by an artificial freezing test, was significantly correlated with field-based measures of seedling (r = 0.64) and growing point (r = 0.84) mortality. The existence of these correlations is of potential interest for the development of indirect selection criteria for complex and expensive-to-evaluate traits such as winter survival in field plots. Besides being reliable, in this study the artificial assessment was also sensitive, providing a greater degree of separation of the populations means than field-based measures. Of the several physiological traits (water content,concentrations of water soluble and total non-structural carbohydrate, and water soluble protein content of stolons) measured at a mid-winter sampling date under field conditions, the only character showing significant variation between populations was soluble protein content. There was a slight trend for material with a higher protein content to exhibit greater field-based values of cold tolerance. High altitude populations tended to have low spring yields. The highest spring yield was found in one of the Ladino populations. The study identified two populations which combined, to differing extents, cold tolerance and spring yield characteristics that would be of potential use in breeding for specified agronomic/climatic zones.
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References
Annicchiarico, P., 1993. Variation for dry matter yield, seed yield and other agronomic traits in Ladino white clover landraces and natural populations. Euphytica 71: 131–141.
Annicchiarico, P. & E. Piano, 1995. Variation within and among ladino white clover ecotypes for agronomic traits. Euphytica 86: 135–142.
Annicchiarico, P., E. Piano & I. Rhodes, 1999. Heritability of, and genetic correlations among, forage and seed yield traits in Ladino white clover. Plant Breed 118: 341–346.
Anonymous, 1998. Grass and Clover. Recommended varieties for Northern Ireland 1998/99. Department of Agriculture for Northern Ireland, Crossnacreevy.
Avice, J.C., A. Ourry, G. Lemaire, J.J. Volenec & J. Boucaud, 1997. Root protein and vegetative storage proteins are key organic nutrients for alfalfa shoot regrowth. Crop Sci 37: 1187–1193.
Boller, B. & J. Nösberger, 1983. Effects of temperature and photoperiod on stolon characteristics, dry matter partitioning, and nonstructural carbohydrate concentration of two white clover ecotypes. Crop Sci. 23: 1057–1062.
Bouchart, V., J.H. Macduff, A. Ourry, M.M. Svenning, A.P. Gay, J.C. Simon & J. Boucaud, 1998. Seasonal pattern of accumulation and effects of low temperatures on storage compounds in Trifolium repens. Physiol Plant 104: 65–74.
Bradford, M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
Caradus, J.R. & B.R. Christie, 1998. Winterhardiness and artificial frost tolerance of white clover ecotypes and selected breeding lines. Can J Plant Sci 78: 251–255.
Caradus, J.R., A.C. MacKay, J. van den Bosch, D.H. Greer & G.S. Wewala, 1989. Intraspecific variation for frost hardiness in white clover. J Agric Sci Camb 111: 151–157.
Castonguay, Y. & A. Guckert, 1996. Adaptation of forage legumes to cold climates. In: G. Parente, J. Frame & S. Orsi (Eds.), Proc 16th European Grassl Fed Mtg, pp. 911–917. ERSA, Gorizia, Italy.
Collins, R.P., 2001. The effect of drought stress and winter stress on the persistence of white clover. In: G. Fisher & B.E. Frankow-Lindberg (Eds.), Proc FAO/CIHEAM Lowland Grasslands Sub-Network Research Conference, La Coruña, 1998, (in press).
Collins, R.P. & I. Rhodes, 1995. Stolon characteristics related to winter survival in white clover. J Agric Sci 124: 11–16.
Collins, R.P., M.J. Glendining & I. Rhodes, 1991. The relationships between stolon characteristics, winter survival and annual yields in white clover (Trifolium repens L.). Grass Forage Sci 46: 51–61.
Collins, R.P., J. Connolly, M. Fothergill, B.E. Frankow-Lindberg, A. Guckert, M.-P. Guinchard, A. Lüscher, J. Nösberger, I. Rhodes, C. Robin, B. Stäheli & S. Stoffel, 1996. Variation in the overwintering and spring growth of white clover cultivars in the cool, wet areas of Europe. In: G. Parente, J. Frame & S. Orsi (Eds.), Proc 16th European Grassl Fed Mtg, pp. 201–204, ERSA, Gorizia, Italy.
Collins, R.P., Á. Helgadóttir & I. Rhodes, 2000. The effects of stolon morphology on the winter survival of white clover in a cool climate. In: Crop development for the cool and wet regions of Europe. Proc Mtg. COST 814 Programme, Pordenone, Italy, (in press).
Davies, W.E. & N.R. Young, 1967. The characteristics of European, Mediterranean and other populations of white clover (Trifolium repens L.). Euphytica 16: 330–340.
Frankow-Lindberg, B.E. & J. Frame, 1996. Developments and challenges in sustainable grassland production. In: G. Parente, J. Frame & S. Orsi (Eds.), Proc 16th European Grassl Fed Mtg, pp. 337–345, ERSA, Gorizia, Italy.
Frankow-Lindberg, B.E. & H.A. von Fircks, 1998. Population fluctuations in three white clover cultivars under cutting, with particular reference to overwintering properties. J Agric Sci Camb 131: 143–153.
Fuller, M.P. & C.F. Eagles, 1978. A seedling test for cold hardiness in Lolium perenne L. J Agric Sci Camb 91: 217–222.
Harris, W., 1987. Population dynamics and competition. In: M.J. Baker & W.M. Williams (Eds.), White Clover, pp. 203–297. CAB International, Wallingford, UK.
Harris, W., I. Rhodes & S.S. Mee, 1983. Observations on environmental and genotypic influences on the overwintering of white clover. J Appl Ecol 20: 609–624.
Junttila, O., M.M. Svenning & B. Solheim, 1990. Effects of temperature and photoperiod on frost resistance of white clover (Trifolium repens) ecotypes. Physiol Plant 79: 435–438.
Li, R., J.J. Volenec, B.C. Joern & S.M. Cunnigham, 1996. Seasonal changes in nonstructural carbohydrates, protein, and macronutrients in roots of alfalfa, red clover, sweetclover, and birdsfoot trefoil. Crop Sci 36: 617–623.
McHugh, S.,M.O. Humphreys, T. McNeilly & M.S. Johnson, 1988. Variation in cold tolerance in Festuca rubra L. Grass Forage Sci 43: 305–312.
Negri, V. & F. Veronesi, 1987. Possible role of natural populations of white clover for reclamation purposes in sub-humid and humid Mediterranean environments. Agronomie 7: 703–708.
Ollerenshaw, J.H. & R. Haycock, 1984. Variation in the low temperature growth and frost tolerance of natural genotypes of Trifolium repens L. from Britain and Norway. J Agric Sci Camb 102: 11–21.
Rhodes, I., R.P. Collins, & D.R. Evans, 1994. Breeding white clover for tolerance to low temperature and grazing stress. Euphytica 77: 239–242.
Robin, C., G. Corbel, M.P. Guinchard, C. Bazard & A. Guckert, 1999. Comportement hivernal et repousse printanière du trèfle blanc: importance du cultivar. Fourrages 157: 21–32.
Røsnes, K., O. Junttila, A. Ernsten & N. Sandli, 1993. Development of cold tolerance in white clover (Trifolium repens L.) in relation to carbohydrate and free amino acid content. Acta Agric Scand 43: 151–155.
Ross, G.J.S., 1987. Maximum Likelihood Program, Release 3.08. Numerical Algorithms Group, Oxford.
Stäheli, B., A. Lüscher & J. Nösberger, 1996. Growth of white clover plants in relation to temperature in winter and spring. In: G. Parente, J. Frame & S. Orsi (Eds.), Proc 16th European Grassl Fed Mtg, pp. 309–312. ERSA, Gorizia, Italy.
Svenning M.M., K. Røsnes & O. Junttila, 1997. Frost tolerance and biochemical changes during hardening and dehardening in contrasting white clover populations. Physiol Plant 101: 31–37.
Turner, L.B. & C.J. Pollock, 1998. Changes in stolon carbohydrates during the winter in four varieties white clover (Trifolium repens L.) of contrasting hardiness. Ann Bot 81: 97–107.
Volenec J.J., A. Ourry & B.C. Joern, 1996. A role for nitrogen reserves in forage regrowth and stress tolerance. Physiol Plant 97: 185–193.
Williams, W.M., 1987. Adaptive variation. In: M.J. Baker & W.M. Williams (Eds.),White Clover, pp. 299–321. CAB International, Wallingford, UK.
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Annicchiarico, P., Collins, R.P., Fornasier, F. et al. Variation in cold tolerance and spring growth among Italian white clover populations. Euphytica 122, 407–416 (2001). https://doi.org/10.1023/A:1012918100232
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DOI: https://doi.org/10.1023/A:1012918100232