Abstract
The Rubus species R. parviflorus, R. spectabilis and R. strigosus interfere with conifer seedling establishment on forest regeneration sites in Canada and the United States. As a first step towards microbial metabolite-based control, callus and cell suspension cultures of the Rubus species were developed as a bioassay system to detect phytotoxic compounds that may have relevance in a vegetation control context. Rapidly growing friable callus and suspension cultures were obtained from leaf disks of the three weedy Rubus species using similar culture media conditions (modified Murashige and Skoog) but required different plant growth regulators (R. parviflorus, 4.5 μM 2,4-D; R. spectabilis, 26.9 μM NAA/0.5 μM zeatin; and R. strigosus, 12.4 μM picloram). Cell growth and health attributes including callus circumference, degree of browning and suspension culture cell viability as measured by the TTC vital stain assay were developed and were rapid and convenient to use. We have established Rubus tissue culture systems that will make it possible for large scale screening of phytotoxic metabolites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson WC (1980) Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta Hortic. 112: 13–20
Borejsza-Wysocki W & Hrazdin G (1994) Establishment of callus and cell suspension cultures of raspberry (Rubus ideaeus cv. Royalty). Plant Cell Tiss. Org. Cult. 37: 213–216
Cantoni L, Berardi G & Rosati P (1993) Callus induction from endosperm culture of blackberry. Acta Hort 352: 145–149
Coates D, Haeussler S & Mather J (1990) A Guide to the response of common plants in British Columbia to management treatments (pp 120–130). FRDA Handbook 008, BC Ministry of Forests & Forestry Canada
Deboo RF & Prasad R (1997) Pesticide use in forest management: The British Columbia experience since 1946. Pestic. Outlook. December. 9–14
Furmanowa M, Glownail K & Syklowska-Baranek K (1997) Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus culture of Taxus media var. Hatfieldii.Plant Cell Tiss. Org. Cult. 49: 75–79
Heller R (1953) Recherche sur la nutrition minerale des tissus vegetaux cultives in vitro. Ann Sci. Nat. Paris 14: 1–223
Hogan ME & Manners GD (1990) Allelopathy of small ever-lasting (Antennaria microphylla): Phytotoxicity to leafy spurge (Euphorbia esula) in tissue culture. J. Chem. Ecol. 16: 931–939
Maier FJ & Oettler G (1992) Selection for the Fusarium toxin deoxynivalenol in callus cultures of triticale. Third European Fusarium Seminar. IHAR Radzikow, September 22–24. 43–49
Mezzetti B, Savini G, Carnevali F & Mott D (1997) Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry. Biol. Plant. 39: 139–150
Mumma RO & Davidonis GH (1983) Plant tissue culture and pesti-cide metabolism. In: Huston DH & Roberts TR (eds) Progress in Pesticide Biochemistry and Toxicology (pp 255–280) John Wiley & Sons, New York
McNicol RJ & Graham J (1990) In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tiss. Org. Cult. 21: 45–50
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nyange NE, Williamson B, McNicol RJ, Lyon GD & Hacket C (1995) In vitro selection of Coffea arabica callus for resistance to partially purified phytotoxic culture filtrates from Colletotrichum kahawae. Ann. Appl. Biol. 127: 425–439
Oleskevich C, Shamoun SF, Vesonder RF & Punja ZK (1998) Evaluation of Fusarium avenaceum and other fungi for potential as biocontrol agents of invasive Rubus species in British Columbia. Can. J. Plant Pathol. 20: 12–18
Owens Y, De Novoa C & Conner AJ (1992) Comparison of in vitro shoot regeneration protocols from Rubus leaf explants. New Zealand J. Crop Hort. Sci. 20: 471–476
Smeda RJ & Weller SC (1991) Plant cell and tissue culture techniques for weed science research. Weed Sci. 39: 497–504
Song HS, Lim SM & Widholm JM (1994) Selection and regener-ation of soybeans resistant to the pathotoxic culture filtrates of Septoria glycines. Phytopathology 84: 948–951
Souissi T & Kremer RJ (1998) A rapid microplate callus bioassay for assessment of Rhizobacteria for biocontrol of leafy spurge. Biocontrol Sci. Tech. 8: 83–92
Taylor HJ, Edwards M, Kiernan RJ, Davey CDM, Blakesley D & Henshaw GG (1996) Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz). N. Biotechnology 14: 726–730
Towill LE & Mazur P (1975) Studies on the reduction of 2,3,5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures. Can. J. Bot. 53: 1097–1102
Wall RE, Prasad R & Shamoun SF (1992) The development and potential role of mycoherbicides for forestry. For. Chronicle. 68: 736–741
Walstad JC & Kuch PJ (ed) (1987) Forest Vegetation Management for Conifer Production. John Wiley & Sons Inc. New York
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hollmann, P.J., Lohbrunner, G.K., Shamoun, S.F. et al. Establishment and characterization of Rubus tissue culture systems for in vitro bioassays against phytotoxins from Rubus fungal pathogens. Plant Cell, Tissue and Organ Culture 68, 43–48 (2002). https://doi.org/10.1023/A:1012915118227
Issue Date:
DOI: https://doi.org/10.1023/A:1012915118227