Abstract
Ultrasound (US) is commonly used as a physiotherapy aid for a number of types of injury to soft connective tissues and for fracture healing. However, the precise effects of therapeutic US on tissue healing processes are not clearly understood, although they are likely to involve changes in key cellular functions. The present study has therefore examined the effects of several US intensity levels on the activity of two bone-associated proteins, alkaline phosphatase (ALP) and osteopontin (OP) in a human cell line, MG63, using RT-PCR. ALP showed progressively higher expression with increasing US intensities, whereas OP responded differently, showing down-regulation at 120 mW/cm2, the lowest US exposure. OP expression was considerably less affected overall compared with the relative response of ALP to the same US doses. The results show that there is a differential response to therapeutic levels of US, since ALP and OP clearly exhibited gene-specific response profiles. These findings suggest that modifying the parameters of US exposure could be used to improve repair and regeneration processes and enhance the clinical efficacy of implanted biomaterials for tissue engineering.
© 2001 Kluwer Academic Publishers
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
G. Ter Haar, M. Dyson and E. M. Oakley, Ultrasound. Med. Biol. 13 (1987) 659.
N. N. Byl, A. L. Mckenzie, J. M. West, J. D. Whitney, T. K. Hunt and H. A. Scheuenstuhl, Arch. Phys. Med. Rehabil. 73 (1992) 656.
C. S. Enwemeka, O. Rodriguez and S. Mendosa, Ultrasound. Med. Biol. 16 (1990) 801.
F. Johannsen, A. N. Gam and T. Karlsmark, Wound. Repair. Regen. 6 (1998) 121.
A. Binder, G. Hodge, A. M. Greenwood, B. L. Hazleman and D. P. P. Thomas, Brit. Med. J. 290 (1985) 512.
J. D. Heckman, J. P. Ryaby, J. Mccabe, J. J. Frey and R. F. Kilcoyne, J. Bone Joint Surg. 76A (1994) 26.
T. K. Kristiansen, J. P. Ryaby, J. Mccabe, J. J. Fray and R. L Roe, J. Bone Joint Surg. 79A (1997) 961.
A. A. Pilla, M. A. Mont, P. R. Nasser, S. A. Khan, M. Figueiredo, J. J. Kaufman and R. S. Siffert, J. Orthop. Trauma. 4 (1990) 246.
M. Dyson and M. Brookes, Ultrasound. Med. Biol. 9 (1983) 61.
F. H. Lin, C. C. Lin, C. M. Lu, H. C. Liu and C. Y. Wang, Med. Eng. Phys. 17 (1995) 20.
M. Tanzer, E. Harvey, A. Kay, P. Morton and J. D. Bobyn, J. Orthop. Res. 14 (1996) 901.
P. Chomczynski and N. Sacchi. Anal. Res. 162 (1987) 156.
D. J. Rickard, M. Kassem, T. E. Hefferan, G. Sarkar T. C. Spelsberg and B. L. Riggs, J. Bone. Min. Res. 11 (1996) 312.
J. Harle, V. Salih, F. Mayia, J. C. Knowles and I. Olsen, Ultrasound in Med. Biol. 27 (2001) 579.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harle, J., Salih, V., Knowles, J.C. et al. Effects of therapeutic ultrasound on osteoblast gene expression. Journal of Materials Science: Materials in Medicine 12, 1001–1004 (2001). https://doi.org/10.1023/A:1012825519114
Issue Date:
DOI: https://doi.org/10.1023/A:1012825519114