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Immunohistochemical expression of the α5 integrin subunit in the normal adult rat central nervous system

  • Published:
Journal of Neurocytology

Abstract

We investigated the distribution of the α5 integrin subunit in the normal adult rat CNS using immunohistochemical methods. Results indicated that the α5 integrin subunit was expressed on the vast majority of neurons throughout the brain and spinal cord. In general, neurons showed diffuse cytoplasmic labelling, although many cortical neurons in layers 4 and 5 did show punctate labelling on the cell surface. In addition, axons within the white matter of the brainstem and caudal CNS areas were labelled, with the most intense labelling seen within the white matter of the spinal cord. In addition, labelling of astrocytes was seen throughout white matter, with particularly heavy astrocyte labelling in the spinal cord. The widespread distribution of the α5 subunit suggests a general function for the α5β1 integrin receptor (the only integrin receptor that includes the α5 subunit) in the adult CNS. The increased expression of fibronectin, the only known ligand for the α5β1 integrin receptor, known to occur around the site of a CNS lesion suggests a possible role for the α5β1 receptor in the response of neurons in the vicinity of a CNS injury.

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Authors and Affiliations

  1. Department of Neuroscience, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK

    Von R. King, Allistair McBride & John V. Priestley

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  1. Von R. King
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  2. Allistair McBride
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  3. John V. Priestley
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Correspondence to Von R. King.

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King, V.R., McBride, A. & Priestley, J.V. Immunohistochemical expression of the α5 integrin subunit in the normal adult rat central nervous system. J Neurocytol 30, 243–252 (2001). https://doi.org/10.1023/A:1012753808599

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