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Ovine Lentivirus is Aetiologically Associated with Chronic Respiratory Disease of Sheep on the Laikipia Plateau in Kenya

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Abstract

A study was undertaken to investigate the occurrence of ovine lentivirus (OvLV) infection in sheep with chronic respiratory disease on the Laikipia Plateau, Kenya. All seven Merino crossbred sheep with chronic dyspnoea and emaciation examined for gross and microscopic lesions had lymphoid interstitial pneumonia (LIP), and one also had pulmonary abscesses. Two of the sheep with LIP also had lesions of ovine pulmonary carcinoma (OPC, jaagsiekte). Using in situ hybridization, OvLV DNA localized to a high proportion of pulmonary macrophages in lungs with lesions of LIP. Lung tissue samples from six of these sheep were positive for a syncytium-inducing virus in cultures of lamb testis cells. Thin-section electron microscopy of infected cells showed virions with morphogenesis typical of lentiviruses. In a western blotting assay, monoclonal antibodies to the OvLV capsid (CA, p27) and matrix (MA, p15) proteins of a North American OvLV isolate reacted with similar-sized bands of the virus, and serum from six of the sheep were reactive with CA from the Kenyan viral isolate. Using an OvLV agar gel immunodiffusion (AGID) test, all seven sheep were positive for serum antiviral antibody, as were 29% of 63 clinically normal sheep from Laikipia District. However, when sera from the healthy sheep were tested in a western blot assay, only 52% had IgG reactive to the OvLV CA, indicating a high rate of false negative reactions with the AGID test. Serum samples from 87 Red Maasai or Dorper crossbred sheep from two farms in other parts of Kenya were OvLV seronegative by both the AGID test and the western blot assay. These results document the first identification of OvLV as a cause of chronic respiratory disease in sheep in Kenya and show a high rate of infection in sheep flocks, with a high prevalence of chronic respiratory disease.

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REFERENCES

  • Brahic, M., Fillipi, P. and Haase, A.T., 1977. Visna virus RNA synthesis. Journal of Virology, 24, 74-81

    Google Scholar 

  • Brodie, S.J., Marcom, K.A., Pearson, L.D., Anderson, B.C., de la Concha-Bermejillo, A., Ellis, J.A. and DeMartini, J.C., 1992. Effects of virus load in the pathogenesis of lentivirus-induced lymphoid interstitial pneumonia. Journal of Infectious Diseases, 166, 531-541

    Google Scholar 

  • Brodie, S.J., Pearson, L.D., Snowder, G.D. and DeMartini, J.C., 1993. Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep. Archives of Virology, 130, 413-428

    Google Scholar 

  • Brodie, S.J., Pearson, L.D., Zink, M.C., Bickle, H.M., Anderson, B.C., Marcom, K.A. and DeMartini, J.C., 1995. Ovine lentivirus expression and disease ‘virus replication, but not entry, is restricted to macrophages of specific tissues.’ American Journal of Pathology, 146, 250-263

    Google Scholar 

  • Brodie, S.J., de la Concha-Bermejillo, A., Snowder, G.D. and DeMartini, J.C., 1998. Current concepts in the epizootiology, diagnosis, and economic importance of ovine progressive pneumonia in North America: a review. Small Ruminant Research, 27, 1-17

    Google Scholar 

  • Brodie, S.J., de la Rosa, C., Howe, G., Travis, W.D. and Diemn, K., 1999. Pediatric AIDS-associated lymphocytic interstitial pneumonia and pulmonary arterio-occlusive disease: role of VCAM-1/VLA-4 adhesion pathway and human herpesviruses. American Journal of Pathology, 154, 1453-1464

    Google Scholar 

  • Coffin, J.M., 1992. Structure and classification of retroviruses. In: J.A. Levy (ed.), The Retroviridae, vol. 1, (Plenum Press, New York), 19-49

    Google Scholar 

  • De Boer, G.F., Terpstra, C., Houwers, D.J., and Hendriks, J., 1979. Studies in epidemiology of maedi/visna in sheep. Research in Veterinary Science, 26, 202-208

    Google Scholar 

  • DeMartini, J.C. and York, D.F., 1997. Retrovirus-associated neoplasms of the respiratory system of sheep and goats. Ovine pulmonary carcinoma and enzootic nasal tumour. Veterinary Clinics of North America Food Animal Practice, 13, 55-70

    Google Scholar 

  • DeMartini, J.C., Rosadio, R.H., Sharp, J.M., Russell, H.I. and Lairmore, M.D., 1987. Experimental coinduction of type D retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs. Journal of the National Cancer Institute, 79, 167-177

    Google Scholar 

  • DeMartini, J.C., Rosadio, R.H. and Lairmore, M.D., 1988. The aetiology and pathogenesis of ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Veterinary Microbiology, 17, 219-236

    Google Scholar 

  • DeMartini, J.C., Brodie, S.J., de la Concha-Bermejillo, A., Ellis, J.A. and Lairmore, M.D., 1993. Pathogenesis of lymphoid interstitial pneumonia in natural and experimental ovine lentivirus infection. Clinical Infectious Diseases, 17, 236-242

    Google Scholar 

  • DeMartini, J.C., de la Concha-Bermejillo, A., Carlson, J.O. and Bowen, R.A., 1999. Diseases caused by maedi-visna and other ovine lentiviruses. In: R.F.E. Axford, S.C. Bishop, F.W. Nicholas and J.B. Owen (eds), Breeding for Disease Resistance in Farm Animals, (CAB International, Oxford), 301-324

    Google Scholar 

  • DeMartini, J.C., Halsey, W., Boshoff, C., York, D. and Howell, M.D., 2000. Comparison of a maedi-visna CA-TM fusion protein ELISA with other assays for detecting sheep infected with North American ovine lentivirus strains. Veterinary Immunology and Immunopathology, 71, 29-40

    Google Scholar 

  • Haase, A.T. and Varmus, H.E., 1973. Demonstration of DNA provirus in the lytic growth of visna virus. Nature (London), 245, 237-239

    Google Scholar 

  • Houwers, D.J., Visscher, A.H. and Defize, P.R., 1989. Importance of ewe/lamb relationship and breed in the epidemiology of maedi-visna virus infections. Research in Veterinary Science, 46, 5-8

    Google Scholar 

  • Katoh, I., Yoshinaka, Y., Rein, A., Shibuya, M., Odaka, T. and Oroszian, S., 1985. Murine leukaemia virus maturation: protease region required for conversion of ‘immature’ to ‘mature’ core form and for virus infectivity. Virology, 145, 280-292

    Google Scholar 

  • Lin, F.H. and Thomas, H., 1970. Characterisation of ribonucleic acid from visna virus. Journal of Virology, 7, 582-587

    Google Scholar 

  • Malmquist, W.A., Krauss, H.H., Moulton, J.E. and Wandera, J.G., 1972. Morphologic study of virus infected lung cell cultures from sheep pulmonary adenomatosis (jaagsiekte). Laboratory Investigation, 26, 528-533

    Google Scholar 

  • Marcom, K.A., Pearson, L.D., Chung, C.S., Poulson, J.M. and DeMartini, J.C., 1991. Epitope analysis of capsid and matrix proteins of North American ovine lentivirus field isolates. Journal of Clinical Microbiology, 29, 1472-1479

    Google Scholar 

  • Marcom, K.A., Brodie, S.J., Pearson, L.D. and DeMartini, J.C., 1992. Analysis of ovine lentivirus infectivity and replication by using a focal immunoassay and an antigen-capture enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 30, 2852-2858

    Google Scholar 

  • Narayan, O., 1989. Lentiviruses are etiological agents of chronic diseases in animals and acquired immunodeficiency syndrome in humans. Canadian Journal of Veterinary Research, 54, 42-48

    Google Scholar 

  • Narayan, O. and Clements, J.E., 1989. Biology and pathogenesis of lentiviruses. Journal of General Virology, 70, 1617-1639

    Google Scholar 

  • Shirlaw, J.F., 1959. Studies on jaagsiekte in Kenya. Bulletin of Epizootic Diseases of Africa, 1, 287-302

    Google Scholar 

  • Sigurdson, B., 1954. Observations on three slow infections of sheep: maedi, paratuberculosis, rida, a slow encephalitis of sheep with general remarks on infections which develop slowly, and some of their special characteristics. British Veterinary Journal, 110, 255-270

    Google Scholar 

  • Sigurdson, B., Thormar, H. and Palsson, P.A., 1960. Cultivation of visna virus in tissue culture. Archives of Virology, 10, 368-381

    Google Scholar 

  • Snyder, S.P., DeMartini, J.C., Ameghino, E. and Caletti, E., 1983. Coexistence of pulmonary adenomatosis and progressive pneumonia in sheep in the Central Sierra of Peru. American Journal of Veterinary Research, 44, 1334-1338

    Google Scholar 

  • Stamatatos, L. and Cheng-Mayer, C., 1993. Evidence that the structural conformation of the envelope gp 120 affects human immunodeficiency virus type 1 infectivity, host range, and syncytium-forming ability. Journal of Virology, 67, 5635-5639

    Google Scholar 

  • Towbin, H., Staehelin, T. and Gordon, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitro-cellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the USA, 76, 4350-4354

    Google Scholar 

  • Vigne, R., Fillipi, P., Querat, G., Sauze, N., Vitu, C., Russo, P. and Delori, P., 1982. Precursor polypeptides to structural proteins of visna virus. Journal of Virology, 42, 1046-1056

    Google Scholar 

  • Wandera, J.G., 1967. Pathology of pneumonia of sheep in Kenya. II. Pathology of chronic progressive pneumonia. Bulletin of Epizootic Diseases of Africa, 15, 393-407

    Google Scholar 

  • Wandera, J.G., 1968. Experimental transmission of sheep pulmonary adenomatosis (jaagsiekte). The Veterinary Record, 83, 478-482

    Google Scholar 

  • Wandera, J.G., 1970. Progressive interstitial pneumonia (maedi) of sheep in Kenya. The Veterinary Record, 86, 434-438

    Google Scholar 

  • Whitworth, S.H., 1926. Observations on a lung affection of lambs and sheep, (Annual Report, Department of Agriculture, Kenya), 102-110

    Google Scholar 

  • Woodward, T.M., Carlson, J.O., de la Concha-Bermejillo, A. and DeMartini, J.C., 1995. Biological and genetic changes in ovine lentivirus strains following passage in isogeneic twin lambs. Journal of Acquired Immune Deficiency Syndromes, 8, 124-133

    Google Scholar 

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Authors and Affiliations

  1. Global Livestock Collaborative Research Support Program (GL-CRSP), Kenya Agricultural Research Institute, Nairobi, Kenya

    P.M. Rwambo

  2. Department of Laboratory Medicine, University of Washington, Seattle, WA, 98195, USA

    S.J. Brodie

  3. Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA

    J.C. DeMartini

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  1. P.M. Rwambo
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  2. S.J. Brodie
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  3. J.C. DeMartini
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Rwambo, P., Brodie, S. & DeMartini, J. Ovine Lentivirus is Aetiologically Associated with Chronic Respiratory Disease of Sheep on the Laikipia Plateau in Kenya. Tropical Animal Health and Production 33, 471–487 (2001). https://doi.org/10.1023/A:1012728528924

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