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Effect of dietary ghee – the anhydrous milk fat on lymphocytes in rats

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Abstract

Lymphocytes are important components of the immune system. Dietary lipids affect the functioning of the immune system. Changes in the lipid composition of the lymphocyte membrane is a case in point. Membrane structural changes are reflected in the altered function of the cell. Lymphocyte proliferation and lymphocyte rosetting are membrane associated phenomena. Ghee, is a clarified butter product, commonly used in the Indian diet. It is rich in saturated fatty acids and also contain oxysterols which are generated on prolonged heating of ghee. Male weanling rats were fed 2.5% (of the total fat levels) of fresh or thermally oxidized ghee for a period of 8 weeks. The control rats were fed groundnut oil. Lipid composition of lymphocytes in ghee fed rats showed changes. In vitro lipid peroxidation of lymphocyte membranes increased by 26% in oxidized ghee fed rats. Na+K+ ATPase activity was decreased in oxidized ghee fed rats (18%). Lymphocyte proliferation was reduced in ghee fed rats (32%), compared to the controls, irrespective of the mitogens used (Con‐A or PHA), or the tissue (splenocytes or peripheral blood lymphocytes). Oxysterols present in oxidized ghee are the likely agents inhibiting lymphoproliferation. Rosetting of lymphocytes decreased in the fresh ghee fed rats by 16% and in oxidized ghee fed rats by 25%. Membrane fluidity declined in the oxidized ghee fed rats. It is concluded that feeding ghee results in decreased proliferation of lymphocytes. Also, feeding oxidised ghee results in decreased proliferation of lymphocytes through alterations in the structure of the lymphocyte membranes in the rat.

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References

  1. Hummel DS: Dietary lipids and immune function. Prog Food Nutr Sci 17: 287–329, 1993

    Google Scholar 

  2. Calder PC: Effects of fatty acids and dietary lipids on cells of the immune system. Proc Nutr Soc 55: 127–150, 1996a

    Google Scholar 

  3. Wiseman H: Dietary influences on membrane functions: Importance in protection against oxidative damage and disease. J Nutr Biochem 7: 2–15, 1996

    Google Scholar 

  4. Kelley DS, Daundu PA: Fat intake and immune response. Prog Food Nutr Sci 17: 41–63, 1993

    Google Scholar 

  5. Yaqoob P, Newsholme EA, Calder PC: The effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation. Immunology 82: 603–610, 1994

    Google Scholar 

  6. Calder Pc, Costa-Rosa LFBP, Curi R: Effects of feeding lipids of different fatty acid compositions upon rat lymphocyte proliferation. Life Sci 56: 455–463, 1995

    Google Scholar 

  7. Yaqoob P, Newsholme EA, Calder PC: The effect of fatty acids on leucocyte subsets and proliferation in rat whole blood. Nutr Res 15: 279–287, 1995

    Google Scholar 

  8. Jeffery NM, Sanderson P, Sherrington EJ, Newsholme EA, Calder PC: The ratio of n-6 to n-3 polyunsaturated fatty acids in the rat diet alters serum lipid levels and lymphocyte functions. Lipids 31: 737–745, 1996a

    Google Scholar 

  9. Niranjan TG, Vijay Kumar M, Lokesh BR, Krishnakantha TP: Influence of unsaponifiable matter from ghee on lymphocyte proliferation and erythrocyte fragility in rats. Nutr Res 19: 1671–1682, 1999

    Google Scholar 

  10. Christ M, Luu B, Mejia JE, Moosbrugger I, Bischoff P: Apoptosis induced by oxysterols in murine lymphoma cells and in normal thymocytes. Immunology 78: 455–460, 1993

    Google Scholar 

  11. Kucuk O, Stoner-Picking J, Yachnin S, Leo IG, Williams RM, Lis LJ, Maxwell PW: Inhibition of cytolytic T-lymphocyte activity by oxysterols. Lipids 29: 657–660, 1994

    Google Scholar 

  12. Jacobson MS: Cholesterol oxides in Indian ghee: Possible causes of unexplained high risk of atherosclerosis in Indian immigrant populations. Lancet (ii): 8560–8656, 1987

    Google Scholar 

  13. Krishnakantha TP, Bischoff P: Immunosuppressive agent in heated ghee? Third International Food Conventions Abstracts, 1993, 91 pp

  14. Hubbel RB, Mendel LB, Wakeman AJ: A new salt mixture for use in experimental diets. J Nutr 14: 273–278, 1937

    Google Scholar 

  15. Kaplay SS: Erythrocyte membrane Na+ and K+ activated adenosine triphosphatase in PCM. J Clin Nutr 31: 579–584, 1978

    Google Scholar 

  16. Ames BN: Assay of inorganic phosphate, total phosphate, and phosphatases. Meth Enzymol 8: 115–118, 1966

    Google Scholar 

  17. Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911–917, 1959

    Google Scholar 

  18. Searcy WM, Bergquist LM: A new colour reaction for the quantitation of serum cholesterol. Clin Chem Acta 5: 192–208, 1960

    Google Scholar 

  19. Stewart JCM: Colorimetric determination of phospholipid with ammonium ferrothiocyanate. Anal Biochem 104: 10–14, 1980

    Google Scholar 

  20. Bina J, Lokesh BR: Prophylactic and therapeutic effects of n-3 polyunsaturated fatty acids, capsaicin, and curcumin on adjuvant induced arthritis in rats. J Nutr Biochem 8: 397–407, 1997

    Google Scholar 

  21. Miller DM, Aust SD: Studies on ascorbate dependent iron catalyzed lipid peroxidation. Arch Biochem Biophys 271: 113–119, 1989

    Google Scholar 

  22. Sedlak J, Lindsay RN: Estimation of total protein band and non protein band sulfhydryl group in tissues with Ellman's reagent. Anal Biochem 25: 192–205, 1968

    Google Scholar 

  23. Yasna S, Yoshiie K, Oda H, Sugano M, Imaizumi K: Dietary Curcuma xanthorrhiza Roxb. increases mitogenic responses of splenic lymphocytes in rats and alters population of the lymphocytes in mice. J Nutr Sci Vitaminol 39: 345–354, 1993

    Google Scholar 

  24. Boyum A: Separation of leukocytes from blood and bone marrow. Can J Clin Lab Invest 21: 77, 1968

    Google Scholar 

  25. Hayam I, Cogan U, Mokady S: Dietary oxidized oil enhances the activity of (Na+K+) ATPase and acetylcholinesterase and lowers the fluidity of rat erythrocyte membrane. J Nutr Biochem 4: 463–568, 1993

    Google Scholar 

  26. Hartree EF: Determination of protein A modification of Lowry method that gives a linear photometric response. Anal Biochem 48: 422–427, 1972

    Google Scholar 

  27. Snedecor GW, Cochran WG: Statistical Methods. The Iowa University Press, Ames, 1977, pp 100–110

    Google Scholar 

  28. Ghafoorunissa, Reddy V, Sesikaran B: Palmolein and groundnut oil have comparable effects on blood lipids and platelet aggregation in healthy Indian subjects. Lipids 30: 1163–1169, 1995

    Google Scholar 

  29. Srivastava S, Joshi CS, Sethi PPS, Agrawal AK, Srivastava SK, Seth PK: Altered platelet functions in non-insulin dependent diabetes mellitus (NIDDM). Thromb Res 76: 451–461, 1994

    Google Scholar 

  30. Hayam I, Uri C, Shoshana M: Dietary oxidized oil enhances the activity of (Na+K+) ATPase and acetylcholinesterase and lowers the fluidity of rat erythrocyte membrane. J Nutr Biochem 4: 563–568, 1993

    Google Scholar 

  31. Kaplan JG: Membrane cation transport and the control of proliferation of mammalian cells. Ann Rev Physiol 40: 19–41, 1978

    Google Scholar 

  32. Jeffery NM, Sanderson P, Newsholme EA, Calder PC: Effects of varying the types of saturated fatty acid in the rat diet upon serum lipid levels and spleen lymphocyte functions. Biochim Biophys Acta 1345: 233–236, 1997

    Google Scholar 

  33. Vijay Kumar M, Sambaiah K, Lokesh BR: Effect of ghee, the anhydrous milk fat on serum and liver lipids in rats. J Nutr Biochem 10: 96–104, 1997

    Google Scholar 

  34. Calder PC, Newsholme EA: Unsaturated fatty acids suppress interleukin-2 production and transferrin receptor expression by concanavalin-A stimulated rat lymphocytes. Mediators Inflamm 1: 107–115, 1992a

    Google Scholar 

  35. Calder PC, Bevan SJ, Newsholme EA: The inhibition of T-lymphocyte proliferation by fatty acids is via an eicosanoid-independent mechanism. Immunology 75: 108–115, 1992

    Google Scholar 

  36. Jolly CA, Jiang YH, Chapkin RS, McMurray DN: Dietary (n-3) polyunsaturated fatty acids suppress murine lymphoproliferation, interleukin-2 secretion, and the formation of diacylglycerol and ceramide. J Nutr 127: 37–43, 1997

    Google Scholar 

  37. Levin G, Cogan U, Mokady S: Riboflavin deficiency and the function and fluidity of rat erythrocyte membranes. J Nutr 120: 857–861, 1990

    Google Scholar 

  38. Stubbs CD, Smith AP: The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim Biophys Acta 779: 89–137, 1984

    Google Scholar 

  39. Hochgraf E, Mokady S, Cogan U: Dietary Oxidized linoleic acid modifies lipid composition of rat liver microsomes and increases their fluidity. J Nutr 127: 681–686, 1997

    Google Scholar 

  40. Balasubramanya NN, Krishnakantha TP: Influence of lyophilized lactic microbes on rosette forming ability of T-lymphocytes from murine blood. Ind J Dairy Sci 5: 12–15, 1994

    Google Scholar 

  41. Hanjan SNS, Talwar GP, Kidwai Z, Nath I: Delineation and quantitation of human peripheral blood lymphocyte subpopulation by electrophoretic mobility and role of surface charge in cell to cell interaction. J Immunol 118: 235–241, 1977

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570 013, India

    T.G. Niranjan & T.P. Krishnakantha

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  1. T.G. Niranjan
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  2. T.P. Krishnakantha
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Correspondence to T.P. Krishnakantha.

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Niranjan, T., Krishnakantha, T. Effect of dietary ghee – the anhydrous milk fat on lymphocytes in rats. Mol Cell Biochem 226, 27–38 (2001). https://doi.org/10.1023/A:1012721332221

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