Abstract
The sensory hair cells of the inner ear are coated with a variety of glycoproteins and glycolipids which can be identified by the binding of specific lectins. The present study examined the binding patterns of three lectins–Wheat Germ Agglutinin, Peanut Agglutinin, and lectin from Griffonia simplicifolia (Isoform B4)–in the avian utricle. Each of the lectins exhibited a distinct pattern of hair cell labeling. Wheat Germ Agglutinin (WGA) appeared to label the ciliary bundles of all sensory hair cells. In contrast, the binding of Peanut Agglutinin (PNA) was mainly confined to the ciliary bundles of extrastriolar hair cells. Finally, lectin from Griffonia simplicifolia (GS-IB4) labeled a subpopulation of hair cells in all regions of the chick utricle. Those bundles were much smaller than the majority of ciliary bundles labeled by either WGA or PNA, and the density of GS-IB4-labeled bundles in the normal mature utricle was relatively low. Increased densities of GS-IB4-labeled hair cells were observed in the embryonic utricle and during the process of hair cell regeneration. The observations suggest that GS-IB4 labels a glycoprotein that is expressed preferentially on the ciliary bundles of immature hair cells.
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Baird, R. A., Schuff, N. R. & Bancroft, J. (1993) Regional differences in lectin binding patterns of vestibular hair cells. Hearing Research 65, 151–163.
de Groot, J. C. M. J. & Veldman, J. E. (1988) Early effects of gentamicin on inner ear glycocalyx cytochemistry. Hearing Research 35, 39–46.
Endo, S. T., Sekitani, H., Yamashita, T., Kido, Y., Masumitsu, M., Ogata, M. & Mirua, M. (1991) Glycoconjugates in the otolithic organ of the developing chick embryo. Acta Otolaryngologica Supplement 481, 116–120.
Forge, A., Souter, M. & Denman-Johnson, K. (1997) Structural development of sensory cells in the ear. Seminars in Cell and Developmental Biology 8, 225–237.
Gil-Loyzaga, P., Raymond, J. & Gabrion, J. (1985) Carbohydrates detected by lectins in the vestibular organs. Hearing Research 18, 269–272.
Gil-loyzaga, P. & Brownell, W. E. (1988) Wheat germ agglutinin and Helix pomatia agglutinin lectin binding on cochlear hair cells. Hearing Research 34, 149–156.
Goodyear, R. J., Gates, R., Lukashkin, A. N. & Richardson, G. P. (1999) Hair-cell numbers continue to increase in the utricular macula of the early post hatch chick. Journal of Neurocytology 28, 863–876.
Goodyear, R. & Richardson, G. P. (1994) Differential glycosylation of auditory and vestibular hair bundle proteins revealed by peanut agglutinin. Journal of Comparative Neurology 345, 267–278.
Hashino, E. & Salvi, R. J. (1996) Regenerated hair cells exhibit a transient resistance to aminoglycoside antibiotics. Brain Research 720, 172–182.
Jacobs, R. A. & Hudspeth, A. J. (1991) Ultrastructural correlates of mechanoelectrical transduction in hair cells of the bullfrog's internal ear. Cold Spring Harbor Symposium on Quantitative Biology 55, 547–561.
JØrgensen, J. M. (1991) Regeneration of lateral line and inner ear vestibular hair cells. In Regeneration of Vertebrate Sensory Receptor Cells (Ciba Foundation Symposium 160, edited by Bock, G. R. & Whelan, J.) pp. 151–163. Chichester: Wiley.
JØrgensen, J. M. (1989) Number and distribution of hair cells in the utricular maculae of some avian species. Journal of Morphology 201, 187–204.
JØrgensen, J. M. & Matheisen, C. (1988) The avian inner ear: Continuous production of hair cells in vestibular sensory organs, but not in the auditory papilla. Naturwissenschaften 75, 319–320.
JØrgensen, J. M. & Andersen, T. (1973) On the structure of the avian maculae. Acta Zoologica 54, 121–130.
Kil, J., Warchol, M. E. & Corwin, J. T. (1997) Ongoing and aminoglycoside-induced hair cell death in the vestibular sensory epithelia of the chicken. Hearing Research 114, 117–126.
Maraen, G. C., Cunningham, D., Burt, J. M., Beecher, M. D. & Rubel, E. W. (1995) Regenerated hair cells in the European starling: Are they more resistant to kanamycin ototoxicity than original hair cells? Hearing Research 82, 267–276.
Matsui, J. I., Ogilvie, J. M. & Warchol, M. E. (2000a) A causative relationship between ongoing hair cell death and supporting cell proliferation in avian vestibular organs. Society for Neuroscience Abstracts 26, 55–15.
Matsui, J. I., Oesterle, E. C., Stone, J. S. & Rubel, E. W. (2000b) Characterization of damage and regeneration in cultured avian utricles. Journal of the Association for Research in Otolaryngology 1, 46–63.
Molliver, D. C., Wright, D. E., Leitner, M. L., Parsadanian, A. S., Doster, K., Wen, D., Yan, D. & Snider, W. D. (1997) IB4-binding DRG neurons switch from NGF to GDNF dependence in early postnatal life. Neuron 19, 849–861.
Neugebauer, D.-Ch. (1986) Interconnections between the stereovilli in the fish inner ear. III. Indications for developmental changes. Cell and Tissue Research 246, 447–453.
Neugebauer, D.-Ch. & Thurm, U. (1985) Interconnections between the stereocilia of the fish ear. Cell and Tissue Research 240, 449–453.
Pickles, J. O., von Perger, M., Rouse, G. W. & Brix, J. (1991) The development of links between steroecilia in hair cells of the chick basilar papilla. Hearing Research 54, 153–163
Pickles, J. O., Comis, S. D. & Osborne, M. P. (1984) Cross-links between stereocilia in the guinea pig organ of Corti, and their possible relation to sensory transduction. Hearing Research 15, 103–112.
Prieto, J. J., Rueda, J., Sada, M. L. & Merchan, J. A. (1990) Lectin staining of saccharides in the normal and hypothyroid developing organ of Corti. Developmental Brain Research 52, 141–149.
Roberson, D. W., Weisleder, P., Bohrer P. S. & Rubel, E. W. (1992) Ongoing production of sensory cells in the vestibular epithelium of the chick. Hearing Research 57, 166–174.
Santi, P. A. & Anderson, C. B. (1987) A newly identified surface coat on cochlear hair cells. Hearing Research 27, 47–65.
Streit, W. J. (1990) An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4). Journal of Histochemistry and Cytochemistry 38, 1683–1686.
Slepecky, N. & Chamberlain, S. C. (1985) The cell coat of inner ear sensory and supporting cells as demonstrated by ruthenium red. Hearing Research 17, 281–288.
Suzuki, H., Katori, Y., Ikeda, K. & Takasaka, T. (1995) Carbohydrate distribution in the living utricular macula of the guinea pig. Hearing Research 87, 32–40.
Takumida, M., Urquiza, R., Bagger-Sjoback, D. & Wersall, J. (1989) Effect of gentamicin on the carbohydrates of the vestibular end organs: An investigation by the use of FITC-lectins. Journal of Laryngology and Otology 103, 357–362.
Takumida, M., Wersall, J. & Bagger-Sjoback, D. (1988) Stereociliary glycocalyx and interconnections in the guinea pig vestibular organs. Acta Otolaryngologica 106, 130–139.
Tilney, L. G., Tilney, M. S. & De Rosier, D. J. (1992) Actin filaments, stereocilia, and hair cells: How cells count and measure. Annual Review of Cell Biology 8, 257–274.
Warchol, M. E. & Corwin, J. T. (1993) Supporting cells in avian vestibular organs proliferate in serum-free medium. Hearing Research 71, 28–36.
Warchol, M. E., Lambert, P. R., Goldstein, B. J., Forge, A. & Corwin, J. T. (1993) Regenerative proliferation in inner ear sensory epithelia from adult guinea pigs and humans. Science 259, 1619–1622.
Warchol, M. E. (1996) Differential lectin binding reveals a subpopulation of hair bundles in avian vestibular organs. Society for Neuroscience Abstracts 22, 636.4.
Weisleder, P. & Rubel, E. W. (1993) Hair cell regeneration after streptomycin toxicity in the avian vestibular epithelium. Journal of Comparative Neurology 331, 97–110.
Wilkins, H. R., Presson, J. C. & Popper, A. N. (1999) Proliferation of vertebrate inner ear supporting cells. Journal of Neurobiology 39, 527–535.
Zheng, J. L. & Gao, W. Q. (1999) Concanavalin A protects hair cells against gentamicin ototoxicity in rat cochlear cultures. Journal of Neurobiology 39, 29–40.
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Warchol, M.E. Lectin from Griffonia simplicifolia identifies an immature-appearing subpopulation of sensory hair cells in the avian utricle. J Neurocytol 30, 253–264 (2001). https://doi.org/10.1023/A:1012705925437
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DOI: https://doi.org/10.1023/A:1012705925437