Nutrient Cycling in Agroecosystems

, Volume 60, Issue 1–3, pp 9–14 | Cite as

Nitrification and denitrification derived N2O production from a grassland soil under application of DCD and Actilith F2

  • P. Merino
  • J.M. Estavillo
  • G. Besga
  • M. Pinto
  • C. González-Murua


The relative contribution of nitrification and denitrification to N2O production was investigated by means of soil incubations with acetylene in a mixed clover/ryegrass sown sward 5 days after application of a mineral fertiliser (calcium ammonium nitrate) or an organic one (cattle slurry) with and without the addition of the nitrification inhibitor dicyandiamide (DCD) and the commercial slurry additive Actilith-F2. At this time, maximum field N2O emissions were taking place. N2O production by the slurry amended soil was twice as high as that of the mineral amended one. N2O came in a greater proportion from nitrification rather than from denitrification in the slurry treatment, while for the mineral fertilisation most N2O came from denitrification. The addition of DCD to slurry produced a decrease in N2O production both from nitrification and denitrification. No reduction in N2O losses was observed from addition of DCD to the mineral fertilisation, although DCD resulted effective in reducing the nitrification rate by 53% both in the slurry and the mineral fertilisation. Actilith F2 induced a high nitrification rate and N2O production from denitrification was reduced while that from nitrification was not.

acetylene inhibition calcium ammonium nitrate cattle slurry nitrification inhibitors nitrification rate 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • P. Merino
    • 1
  • J.M. Estavillo
    • 2
  • G. Besga
    • 3
  • M. Pinto
    • 3
  • C. González-Murua
    • 2
  1. 1.Dpto. Biología Vegetal y EcologíaUPV/EHUBilbao, BizkaiaSpain (Corresponding author)
  2. 2.Dpto. Biología Vegetal y EcologíaUPV/EHUBilbao, BizkaiaSpain
  3. 3.NEIKER, BoDerio, BizkaiaSpain

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