Abstract
Resistance to rice virus diseases is an important requirement in many Southeast Asian rice breeding programs. Inheritance of resistance to rice tungro spherical virus (RTSV) in TW5, a near-isogenic line derived from Indonesian rice cultivar Utri Merah, was compared to that in TKM6, an Indian rice cultivar. Both TKM6 and Utri Merah are cultivars resistant to RTSV infections. Crosses were made between TKM6 and TN1, a susceptible cultivar, and between TW5 and TN1, and F3 lines were evaluated for their resistance to RTSV using two RTSV inoculum sources and a serological assay (ELISA). In TKM6, the resistance to the mixture of RTSV-V + RTBV inoculum source was controlled by a single recessive gene, whereas in TW5, the resistance was controlled by two recessive genes. A single recessive gene, however, controlled the resistance in TW5 when another RTSV variant, RTSV-VI, was used, suggesting that the resistance in TW5 depends on the nature of the RTSV inoculum used. RT-PCR, sequence, and phylogenetic analyses confirmed that RTSV-VI inoculum differs from RTSV-V inoculum and accurate phenotyping of the resistance to RTSV requires the use of a genetic marker.
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Azzam, O., L. Ma, M. Yambao, M. Muhsin, K. McNally & K. Umadhay, 2000a. Genetic diversity of rice tungro spherical virus in tungro-endemic provinces of the Philippines and Indonesia. Arch of Virol 145: 1183-1197.
Azzam, O., R. Cabunagan & T.C.B. Chancellor (Eds.), 2000b. Methods for Evaluating Resistance to Rice Tungro Disease. IRRI Discussion Paper 38.
Bajet, N. B., R.D. Daquiaoag & H. Hibino, 1985. Enzyme-linked immunosorbent assay to diagnose rice tungro. J Plant Prot Trop 2: 25-129.
Cabauatan P.Q., R.C. Cabunagan & H. Koganezawa, 1995. Biological variants of rice tungro viruses in the Philippines. Phytopathology 85: 77-81.
Cabunagan, R.C., E.R. Angeles, S. Villareal, O. Azzam, P.S. Teng, G.S. Khush, T.C.B. Chancellor, E.R. Tiongco, X.H. Truong, S. Mancao, I.G.N. Astika, A. Muis, A.K. Chowdhury, V. Narasimhan, T. Ganapathy & N. Subramanian, 1999. Multilocation evaluation of advanced breeding lines for resistance to rice tungro viruses. In: T.C.B. Chancellor, O. Azzam & K.L. Heong (Eds.), Rice Tungro Disease Management, pp 45-55. Manila, Philippines: International Rice Research Institute.
Dahal G., H. Hibino, R.C. Cabunagan, E.R. Tiongco, Z.M. Flores & V.M. Aguiero, 1990. Changes in cultivar reactions to tungro due to changes in the ‘virulence’ of the leafhopper vector. Phytopathology 80: 659-665.
Dahal G., I. Dasgupta, G. Lee & R. Hull, 1992. Comparative transmission of, and varietal reaction to, three isolates of rice tungro disease. Ann Appl Biol 120: 287-300.
Ebron, L.A., R.R. Yumol, R. Ikeda & T. Imbe, 1994. Inheritance of resistance to rice tungro spherical virus in some rice cultivars. Int Rice Res Notes 19: 10-11.
Fraser, R.S.S., 1992. The genetics of plant-virus interactions: implications for plant breeding. Euphytica 63: 175-185.
Hibino, H., 1983. Relations of rice tungro bacilliform and rice tungro spherical virus with their vector Nephotettix virescens. Ann Phytopathol Soc Jpn 49: 545-553.
Hibino, H., 1995. Rice tungro-associated viruses and their relationships to host plants and vector leafhoppers. In: P. Rudra, Uma S. Singh, & K. Kohmoto (Eds.), Pathogenesis and Host Specificity in Plant Diseases: Histopathological, Biochemical, and Molecular Bases. Vol. III. 1st ed, pp 393-402. Elsevier Science Ltd, Oxford, U.K.
Hibino H., E.R. Tiongco, R.C. Cabunagan & Z.M. Flores, 1987. Resistance to rice tungro-associated viruses in rice under experimental and natural conditions. Phytopathology 77: 871-875.
Imbe, T., 1995a. Genetic Analysis for Resistance to Tungro. Program Report for 1994. IRRI, Los Baños, Philippines.
Imbe, T., 1995b. The Development of Stabilization Technology for Rice Double Cropping in the Tropics. Final Report. IRRI-Government of Japan Collaborative Research Project (December 1989–November 1994).
International Network for Genetic Evaluation of Rice (INGER), 1996. Standard Evaluation System for Rice. 4th edition. Los Baños, Laguna. International Rice Research Institute, The Philippines.
Inoue H. & S. Ruay-Aree, 1977. Bionomics of green leafhopper and epidemics of yellow orange leaf virus disease in Thailand. Trop Agric Res Ser 10: 117-121.
Isogai, M., P.Q. Cabauatan, C. Masuta, I. Uyeda & O. Azzam, 2000. Complete nucleotide sequence of rice tungro spherical virus genome of a highly virulent strain Vt6. Virus Genes 20(1): 75-81.
Khush G.S. & S.S. Virmani, 1985. Breeding rice for disease resistance. In: G.E. Russell (Ed.), Progress in Plant Breeding I, pp. 239-279. Blackwell Scientific Publications, Oxford.
Koganezawa, H. & R.C. Cabunagan, 1997. Resistance to rice tungro virus disease. In: T.C.B. Chancellor & J.M. Thresh (Eds.), Epidemiology and Management of Rice Tungro Disease. Chatham, UK: Natural Resources Institute.
Manwan I., S. Sama & S.A. Rizvi, 1985. Use of varietal rotation in the management of tungro disease in Indonesia. Indonesian Agric Res Dev J 7: 43-48.
Mayo M.A. & C.R. Pringle, 1998. Virus taxonomy -- 1997. J Gen Virol 79: 649-657.
Nemoto, H. & H. Habibuddin, 1992. Studies on breeding for resistance to rice tungro virus in Malaysia. Tropical Agriculture Research Center, Japan/Malaysian Agricultural Research and Development Institute, Malaysia.
Shen P., M. Kaniewska, C. Smith & R.N. Beachy, 1993. Nucleotide sequence and genomic organization of rice tungro spherical virus. Virology 193: 621-630.
Suh, S.K., S.K. Green & H.G. Park, 1995. Genetics of resistance to five strains of turnip mosaic virus in Chinese cabagge. Euphytica 81: 71-77.
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Azzam, O., Imbe, T., Ikeda, R. et al. Inheritance of resistance to rice tungro spherical virus in a near-isogenic line derived from Utri Merah and in rice cultivar TKM6. Euphytica 122, 91–97 (2001). https://doi.org/10.1023/A:1012605218343
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DOI: https://doi.org/10.1023/A:1012605218343