Abstract
We showed previously that Epstein–Barr virus (EBV) latency is disrupted and the virus-replicative cycle is activated after expression of EBNA2 in the Burkitt's lymphoma-derived Akata cells. Here, an EBNA2 deletion mutant lacking the amino acid residues 248–382, including the region responsible for association with RBP-J κ, was generated and tested for its ability to activate EBV replication in Akata cells. This mutant was shown clearly deficient in inducing the EBV-replicative cycle, suggesting that association with RBP-J κ is necessary for the EBV activating function of EBNA2. It is thus likely that EBV activation by EBNA2, seemingly in conflict with its involvement in lymphocyte immortalization, is nevertheless based on the standard mechanism of transactivation by the protein.
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Kieff E., Epstein-Barr virus and its replication, in Fields B.N., Knipe D.M., Howley P.M., Chanock R.M., Melnick J.L., Monath T.P., Roizman B., and Straus S.E. (eds), Virology, 2nd edn. Lippincott-Raven, Philadelphia, 1996, pp. 2343–2396.
Rickinson A.B. and Kieff E., Epstein-Barr virus, in Fields B.N., Knipe D.M., Howley P.M., Chanock R.M., Melnick J.L., Monath T.P., Roizman B., and Straus S.E. (eds), Virology, 2nd edn. Lippincott-Raven, Philadelphia, 1996, pp. 2397–2446.
Hammerschmidt W. and Sugden B., Nature 340, 393–397, 1989.
Cohen J., Wang F., Mannick J., and Kieff E., Proc Natl Acad Sci USA 86, 9558–9562, 1989.
Kempkes B., Spitkovsky D., Jansen-Durr P., Ellwart J.W., Kremmer E., Delecluse H.-J., Rottenberger C., Bornkamm G.W., and Hammerschmidt W., EMBO J 14, 88–96, 1995.
Sung N.S., Kenney S., Gutsch D., and Pagano Y.S., J Virol 65, 2164–2169, 1991.
Jin X.W. and Speck S.H., J Virol 66, 2846–2852, 1992.
Walls D. and Perricaudet M., EMBO J 110, 143–151, 1991.
Abbot S.D., Rowe M., Cadwallader K., Ricksten A., Gordon J., Wang F., Rymo L., and Rickinson A.B., J Virol 64, 2126–2134, 1990.
Fahraeus R., Jansson A., Ricksten A., Sjoblom A., and Rymo L., Proc Natl Acad Sci USA 87, 7390–7394, 1990.
Wang F., Tsang S.-F., Kurilla M.G., Cohen J.I., and Kieff E., J Virol 64, 3407–3416, 1990.
Zimber-Strobl U., Suentzenich K.O., Laux G., Eick D., Cordier M., Calender A., Billaud M., Lenoir G.M., and Bornkamm G.W., J Virol 65, 415–423, 1991.
Wang F., Gregory C., Sample C., Row M., Liebowitz D., Murray R., Rickinson A., and Kieff E., J Virol 64, 2309–2318, 1990.
Wang F., Gregory C.D., Rowe M., Rickinson A.B., Wang D., Birkenbach M., Kikutani H., Kishimoto T., and Kieff E., Proc Natl Acad Sci USA 83, 3452–3457, 1987.
Kaiser C., Laux G., Eick D., Jochner N., Bornkamm GW., and Kempkes B., J Virol 73, 4481–4484, 1999.
Knutson J.C., J Virol 64, 2530–2536, 1990.
Grossman S.R., Johannsen E., Tong X., Yalamanchili R., and Kieff E., Proc Natl Acad Sci USA 91, 7568–7572, 1994.
Henkel T., Ling P.D., Hayward S.D., and Petersen M.G., Science 265, 92–95, 1994.
Hsieh J.J.-D. and Hayward S.D., Science 268, 560–563, 1995.
Zimber-Strobl U., Strobl L.J., Meitinger C., Hinrichs R., Sakai T., Furukawa T., Honjo T., and Bornkamm G.W., EMBO J 13, 4973–4982, 1994.
Waltzer L., Logeat F., Brou C., Israel A., Sergeant A., and Manet E., EMBO J 13, 5633–5638, 1994.
Jayachandra S., Low K.G., Thlick A.-E., Yu J., Ling P.D., Chang Y., and Moore P.S., Proc Natl Acad Sci USA 96, 11566–11571, 1999.
Takada K., Horinouchi K., Ono Y., Aya T., Osato T., Takahashi M., and Hayasaka S., Virus Genes 5, 147–156, 1991.
Gossen M. and Bujard H., Proc Natl Acad Sci USA 89, 5547–5551, 1992.
Schokett P., Difilippantonio M., Hellman N., and Schatz D.G., Proc Natl Acad Sci USA 92, 6522–6526, 1995.
Fujiwara S., Nitadori Y., Nakamura H., Nagaishi T., and Ono Y., J Virol 73, 5214–5219, 1999.
Young L., Alfieri C., Hennessy K., Evans H., O'Hara C., Anderson K.C., Ritz J., Shapiro R.S., Rickinson A., Kieff E., and Cohen J.I., N Engl J Med 321, 1080–1085, 1989.
Johannsen E., Koh E., Mosialos G., Tong X., Kieff E., and Grossman S.R., J Virol 69, 253–262, 1995.
Cordier M., Calender A., Billaud M., Zimber U., Rousselet G., Pavlish O., Banchereau J., Tursz T., Bornkamm G., and Lenoir G.M., J Virol 64, 1002–1013, 1990.
Le Roux A., Kerdiles B., Walls D., Dadieu J.F., and Perricaudet M., Virology 205, 596–602, 1994.
Johannsen E., Miller C.L., Grossman S.R., and Kieff E., J Virol 70, 4179–4183, 1996.
Radkov S.A., Bain M., Farrell P.J., West M., Rowe M., and Allday M., J Virol 71, 8552–8562, 1997.
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Fujiwara, S., Liu, E. & Shimizu, K. The Amino Acid Region 248–382 of the Epstein–Barr Virus Nuclear Protein 2 (EBNA2) is Responsible for the EBNA2-Induced EBV Reactivation. Virus Genes 23, 361–365 (2001). https://doi.org/10.1023/A:1012585709642
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DOI: https://doi.org/10.1023/A:1012585709642