Abstract
A novel ‘nano’-tool based on metal clusters enables the transduction of changes of biorecognitive binding as well as conformation quantitatively into an optical signal which can be observed directly as a color change of a sensor-chip surface.
Proteins including various enzymes and serum proteins were spotted via micro-arraying onto the chip surface forming monolayer and thin film dots. Multi-layered (up to 300 nm thick) nano-gel-pads were stabilized by photo crosslinking the protein dots with UV-light. By deposition of metal-nano clusters, synthesized via chemical means or sputter coating on top of photo-crosslinked macromolecules an optical resonance effect was obtained.
The response of the novel surface enhanced absorption cluster sensor-chip was transduced spectroscopically in the visible and IR range of the spectrum via an 8 μm resolution reflectance scanner.
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Mayer, C., Palkovits, R., Bauer, G. et al. Surface Enhanced Resonance of Metal Nano Clusters: A Novel Tool for Proteomics. Journal of Nanoparticle Research 3, 359–369 (2001). https://doi.org/10.1023/A:1012564029052
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DOI: https://doi.org/10.1023/A:1012564029052