Abstract
The effect of cell density (1–4.5 g L-1) and light intensity (44 and 82 μmol m-2 s-1) on fatty acid composition andeicosapentaenoic acid (EPA, 20:5 ω3) production was studied ina semi-continuous culture of Monodus subterraneus grown in a helicaltubular photobioreactor (`Biocoil') under laboratory conditions. Under lowlight, the highest proportion of EPA (31.5% of total fatty acids) and EPAcontent (3.5% of dry weight), biomass productivity (1.3 g L-124 h-1) and EPA productivity (44 mg L-1 24 h-1)occurred at optimal cell density of about 1.7 g L-1. Cell densityhad no effect on the total fatty acid (TFA) content and was maintained atca. 11% of dry weight. Under high light, the highest proportion ofEPA to fatty acids (31.8%), the total fatty acids content (13.4%) andEPA content (4.3% of dry weight) occurred at cell density of about 3.4gL-1. But the highest biomass productivity (1.7 g L-124 h-1) and EPA productivity (56 mg L-1 24 h-1) wereobtained at a cell density of 1.6 and 2.6g L-1, respectively. Ourresults suggest that manipulating the cell density and light intensity canmodify the composition of fatty acid and production of eicosapentaenoicacid (EPA) in M. subterraneus.
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Lu, C., Rao, K., Hall, D. et al. Production of eicosapentaenoic acid (EPA) in Monodus subterraneus grown in a helical tubular photobioreactor as affected by cell density and light intensity. Journal of Applied Phycology 13, 517–522 (2001). https://doi.org/10.1023/A:1012515500651
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DOI: https://doi.org/10.1023/A:1012515500651