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Targeted gene disruption of the avermectin B O-methyltransferase gene in Streptomyces avermitilis

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Abstract

The biological activity of avermectin B components is superior to that of avermectin A components, which are derived from avermectin B by avermectin B 5-O-methyltransferase. Gene disruption, targeting avermectin B 5-O-methyltransferase gene in Streptomyces avermitilis, was carried out to obtain a strain of avermectin B producer. Phenotype analysis of the mutant with the disrupted O-methyltransferase gene showed that only avermectin B components were produced with a significant increase in production

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Authors and Affiliations

  1. Korea Research Institute of Bioscience and Biotechnology, P.O. Box, 115, Yusong, Taejon, 305-600, Korea

    Young-Soo Hong, Jeong Hyung Lee, Hang Sub Kim & Jung Joon Lee

  2. Department of Molecular Biology, Pusan National University, Pusan, 609-735, Korea

    Kyu-Won Kim

Authors
  1. Young-Soo Hong
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  2. Jeong Hyung Lee
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  3. Hang Sub Kim
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  4. Kyu-Won Kim
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  5. Jung Joon Lee
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Hong, YS., Lee, J.H., Kim, H.S. et al. Targeted gene disruption of the avermectin B O-methyltransferase gene in Streptomyces avermitilis. Biotechnology Letters 23, 1765–1770 (2001). https://doi.org/10.1023/A:1012496317299

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