Skip to main content
SpringerLink
Log in

Production of gluconic acid by immobilized in pumice stones mycelium of Aspergillus niger using unconventional oxygenation of culture

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Gluconic acid was produced in repeated batch processes with Aspergillus niger AM-11, immobilized in pumice stone particles using an unconventional oxygenation of culture media based on the addition of H2O2, decomposed by catalase to O2 and water. The highest gluconic acid productivity of 8.2 g l−1 h−1 was reached with 30 g immobilized mycelium per 150 ml, 10% (w/v) glucose, at 24 °C and pH 6.5, with O2 at 100% saturation. The immobilized mycelium was successfully reused up to 8 times in 1-h batches with only a slight loss (11%) of gluconic acid productivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Attwood MM, Van Dijken JP, Pronk JT (1991) Glucose metabolism and gluconic acid production by Acetobacter diazotrophicus. J. Ferment. Bioeng. 72: 101–105.

    Google Scholar 

  • Bailey JE, Ollis DF (1986) Biochemical Engineering Fundamentals, 2nd edn. New York: McGraw-Hill.

    Google Scholar 

  • Dronawat SN, Shihla CK, Hanley TR (1995) The effect of agitation and aeration on the production of gluconic acid by Aspergillus niger. Appl. Biochem. Biotechnol. 51/52: 347–354.

    Google Scholar 

  • Fiedurek J, Gromada A (2000) Production of catalase and glucose oxidase using unconventional oxygenation of culture. J. Appl. Microbiol. 89: 85–89.

    PubMed  Google Scholar 

  • Fiedurek J, Ilczuk Z (1991) Glucose oxidase biosynthesis using immobilized mycelium of Aspergillus niger. World J. Microbiol. Biotechnol. 7: 379–384.

    Google Scholar 

  • Fiedurek J, Pielecki J (1999) Apparatus for controlling dissolved oxygen concentration in the medium. Polish Patent No. P331–193, 3.02.1999.

  • Gromada A, Fiedurek J (1997) Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production. J. Appl. Microbiol. 82: 648–652.

    PubMed  Google Scholar 

  • Lloyd JB, Whelan W (1969) An improved method for enzymatic determination of glucose in the presence of maltose. Anal. Biochem. 30: 467–470.

    PubMed  Google Scholar 

  • Nidetzky B, Fuerlinger M, Gollhofer D, Scopes RK, Haltrich D, Kulbe KD (1997) Improved operational stability of cell-free glucose-fructose oxidoreductase from Zymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor. Biotechnol. Bioeng. 53: 623–630.

    Google Scholar 

  • Oosterhuis NMG, Kossen NWF (1983) Oxygen transfer in a production scale bioreactor. Chem. Eng. Res. Des. 61: 308–312.

    Google Scholar 

  • Röper M (1991) Selective oxidation of D-glucose. In: Lichtenthaler FW, ed. Chiral Intermediates for Industrial Utilization in Carbohydrates as Organic Raw Materials. Weinheim: VCH Verlagsgesellschaft GmhB, pp. 273–274.

    Google Scholar 

  • Träger M, Qazi GN, Buse R, Onken U (1992) Influence of constant and oscillating dissolved oxygen concentration on Aspergillus niger. J. Ferment. Bioeng. 74: 282–287.

    Google Scholar 

  • Träger M, Qazi GN, Onken U, Chopra CL (1991) Contribution of endo and exocellular glucose oxidase to gluconic acid production at increased dissolved oxygen concentrations. J. Chem. Technol. Biotechnol. 50: 1–11.

    Google Scholar 

  • Velizarow S, Beschkov V (1994) Production of free gluconic acid by cells of Gluconobacter oxydans. Biotechnol. Lett. 16: 715–720.

    Google Scholar 

  • Witteveen CFB, Vondervoort P, Swart K, Visser J (1990) Glucose oxidase overproducing and negative mutants of Aspergillus niger. Appl. Microbiol. Biotechnol 33: 683–686.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Jan Fiedurek

Authors
  1. Jan Fiedurek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jan Fiedurek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fiedurek, J. Production of gluconic acid by immobilized in pumice stones mycelium of Aspergillus niger using unconventional oxygenation of culture. Biotechnology Letters 23, 1789–1792 (2001). https://doi.org/10.1023/A:1012461200152

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1012461200152

Search

Navigation