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Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

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Abstract

Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g−1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.

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Authors and Affiliations

  1. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China

    Yong-Gang Zheng, Yong Li, Jian-Feng Chen & En-Duo Wang

  2. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

    Wei-Hong Jiang & Guo-Ping Zhao

Authors
  1. Yong-Gang Zheng
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  2. Yong Li
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  3. Jian-Feng Chen
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  4. Wei-Hong Jiang
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  5. Guo-Ping Zhao
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  6. En-Duo Wang
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Zheng, YG., Li, Y., Chen, JF. et al. Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase. Biotechnology Letters 23, 1781–1787 (2001). https://doi.org/10.1023/A:1012452516082

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