Abstract
Sugar cane bagasse was subjected to a mixed culture, solid substrate fermentation with Trichoderma reesei QM9414 and Aspergillus terreus SUK-1 to produce cellulase and reducing sugars. The highest cellulase activity and reducing sugar amount were obtained in mixed culture. The percentage of substrate degradation achieved employing mixed culture was 26% compared to 50% using separate cultures of the two molds. This suggests that the synergism of enzymes in mixed culture solid substrate fermentation have lower synergism than in pure culture.
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Castillo MR, Gutierrez-Correa M, Linden JC, Tengerdy RP (1994) Mixed culture solid substrate fermentation for cellulolytic enzyme production. Biotechnol. Lett. 16: 967–972.
Duenas R, Tengerdy RP, Gutierrez-Correa M (1995) Cellulase production by fungi in solid-substrate fermentation of bagasse. World J. Microbiol. Biotechnol. 11: 333–337.
Duff SJB, Cooper DG, Fuller ON (1987) Effect of media composition and growth conditions on production of cellulase and β-glucosidase by a mixed fungal fermentation. Enzyme Microb. Technol. 9: 47–52.
Gutierrez-Correa M, Tengerdy RP (1997) Production of cellulase on sugar cane bagasse by fungal mixed culture solid substrate fermentation. Biotechnol. Lett. 19: 665–667.
Mandels M, Andreotti R, Roche C (1976) Measurement of saccharifying cellulase. Biotechnol. Bioeng. Symp. 6: 21–33.
Muniswaran PKA, Charyulu NCLN (1994) Solid substrate fermentation of coconut coir pith for cellulase production. Enzyme Microb. Technol. 16: 436–439.
Ryu DY, Mandels M (1980) Cellulases: biosynthesis and applications. Enzyme Microb. Technol. 2: 91–102.
Sternberg D (1976) Production of cellulase by Trichoderma. Biotechnol. Bioeng. 6: 35–53.
Wan Mohtar WY, Thayan R (1991) Synergism of β-glucosidase and cellulase in mixed culture fermentations. Malays Appl. Biol. 20: 215–222.
Wan Mohtar WY, Muhannad IM, Othman O, Jalil K (2000) Sugar cane baggase degradation by mixed culture of T. reesei and A. terreus in solid substrate fermentation. Pakistan J. Biol. Sci. 3: 1758–1761.
Wood TM, Bhat KM (1988) Methods of measuring cellulase activities. Meth. Enzymol. 160: 87–112.
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Massadeh, M.I., Mohtar Wan Yusoff, W., Omar, O. et al. Synergism of cellulase enzymes in mixed culture solid substrate fermentation. Biotechnology Letters 23, 1771–1774 (2001). https://doi.org/10.1023/A:1012448401369
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DOI: https://doi.org/10.1023/A:1012448401369