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Overproduction of pyoverdins by Fur mutants of Pseudomonas aeruginosa strains PAO1 and Fe10 in stirred bioreactors

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Abstract

Fur mutants FPA12 and FF13 of strains Pseudomonas aeruginosa PAO1 and Fe10, respectively, were prepared and their production of pyoverdin evaluated. The strains were cultivated in stirred bioreactor in iron-deficient and iron-supplemented medium containing Casamino acids (CA) or succinate as a source of carbon and energy. When the pyoverdin production rate reached its maximum, the demand of iron-depleted cultures for O2 was decreased. Mutant FF13 overproduced pyoverdin in both iron-depleted (862 mg l−1) and iron-supplemented (428 mg l−1) CA medium and could also be used to produce pyoverdin when grown in a conventional stirred tank fermenter.

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Authors and Affiliations

  1. Laboratory of Enzyme Technology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic

    Andrea Johnová, Magda Dobišová & Pavel Kyslík

  2. Laboratoire de Chimie Microbienne, Departement des Recepteurs et Proteines Membranaires, Ecole Superieure de Biotechnologie de Strasbourg, UPR 9050 C.N.R.S., 67400, Illkirch, France

    Mohamed A. Abdallah

Authors
  1. Andrea Johnová
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  2. Magda Dobišová
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  3. Mohamed A. Abdallah
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  4. Pavel Kyslík
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Johnová, A., Dobišová, M., Abdallah, M.A. et al. Overproduction of pyoverdins by Fur mutants of Pseudomonas aeruginosa strains PAO1 and Fe10 in stirred bioreactors. Biotechnology Letters 23, 1759–1763 (2001). https://doi.org/10.1023/A:1012444300461

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