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Studies on the molecular ecology of Blastomyces dermatitidis

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Abstract

The microecology of Blastomyces dermatitidis, the dimorphic etiologic agentof the potentially fatal systemic fungal infection, blastomycosis, is not well defined.Blastomyces dermatitidis may occur periodically at natural sites, perhaps aided by rotting organic material, animal droppings and physical changes. Semi-quantitative growth studies of B. dermatitidis on 2% agar plates determined the ability toutilize or tolerate a variety of substrates including simple and complex molecules as carbon source, and organic and inorganic nitrogen sources. Allantoin, creatinine, quanidoacetic acid, guanidine and cysteine may be used as sole nitrogen source. Allantoin in combination with dextrose, glycerol, lichenen, celloboise and other wood by-products support growth of B. dermatitidis at room temperature. The nutritional conversion of the fungus to the yeast form at room temperature, well demonstrated on allantoin/glycerol/yeast extract media, appears to be affected by certain inorganic compounds. The organism tolerates low to moderate levels of alpha-pinene, tannic acid, shikimic acid, veratryl alcohol, vanillic acid, and polyethyleneglycol-200. There are significant differences among isolates regarding growth on various substances at 20° and 37° centigrade. It appears that a variety of wood by-products and animal waste substrates, in combination, support the growth of B. dermatitidis. Their role in the ecological niche of B. dermatitidis, and the importance of nutritional dimorphism in the natural environment warrants further investigation.

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Authors and Affiliations

  1. Department of Family Medicine, University of Wisconsin Medical School, Milwaukee, WI, USA

    Dennis J. Baumgardner

  2. University of Wisconsin Medical School, Madison, WI, USA

    Bryan Laundre

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  1. Dennis J. Baumgardner
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Baumgardner, D.J., Laundre, B. Studies on the molecular ecology of Blastomyces dermatitidis . Mycopathologia 152, 51–58 (2001). https://doi.org/10.1023/A:1012438029997

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