Abstract
Under the condition that the longitudinal relaxation time of spin I is shorter than the longitudinal relaxation time of spin S the steady-state magnetization in [S,I]-TROSY-type experiments can be enhanced by intermediate storage of a part of the steady-state magnetization of spin I on spin S with a pulse sequence element during the relaxation delay. It is demonstrated with samples ranging in size from the 1 kDa cyclosporin to the 110 kDa 15N,2H-labeled dihydroneopterin Aldolase that intermediate storage of steady-state magnetization in a [15N,1H]-TROSY experiment yields a signal gain of 10–25%. The method proposed here for intermediate storage of steady-state magnetization can be implemented in any [15N,1H]-TROSY-type experiments.
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Riek, R. Enhancement of the steady-state magnetization in TROSY experiments. J Biomol NMR 21, 99–105 (2001). https://doi.org/10.1023/A:1012431013082
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DOI: https://doi.org/10.1023/A:1012431013082