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Increased α3-fucosylation of α1-acid glycoprotein in Type I diabetic patients is related to vascular function

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Abstract

Diabetic mellitus is attended by the development of endothelial dysfunction which is suggested to be accompanied with a chronic low-degree of inflammation. During a chronic hepatic inflammatory response, specific changes in glycosylation of the acute phase protein α1-acid glycoprotein (AGP) can be detected. In this report we studied the changes in glycosylation of AGP in more detail and evaluated the relation between a change in glycosylation of AGP and urinary albumin secretion in Type I diabetic patients. The glycosylation of AGP, studied by crossed affinity immunoelectrophoresis (CAIE) and high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD), showed an increase in α3-fucosylation. Staining with an antibody against sialyl Lewisx (sLex) implied that part of the α3-fucosylation was present in a sLex-conformation. In the group of Type I diabetic patients with increased urinary albumin excretion, a significant increase in α3-fucosylation of AGP (p[emsp4 ]<[emsp4 ]0.0005) could be detected. Therefore, the increased α3-fucosylation of AGP can be used as an additional marker for the development of vascular complications in Type I diabetic patients.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, Glycoimmunology Group, Institute for Immunology and Inflammatory diseases, Faculty of Medicine, VU Medical Center, Amsterdam, The Netherlands

    Dennis C. W. Poland, Casper G. Schalkwijk, Carolien A. M. Koeleman, Bert van het Hof & Willem van Dijk

  2. Internal Medicine, Institute for Cardiovascular Research, VU Medical Center, Amsterdam, The Netherlands

    Coen D. A. Stehouwer

Authors
  1. Dennis C. W. Poland
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  2. Casper G. Schalkwijk
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  3. Coen D. A. Stehouwer
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  4. Carolien A. M. Koeleman
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  5. Bert van het Hof
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  6. Willem van Dijk
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Poland, D.C.W., Schalkwijk, C.G., Stehouwer, C.D.A. et al. Increased α3-fucosylation of α1-acid glycoprotein in Type I diabetic patients is related to vascular function. Glycoconj J 18, 261–268 (2001). https://doi.org/10.1023/A:1012412908983

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