Abstract
The glutaminase (EC 3.5.1.2) isolated from seedlings of triticale (Triticalesp.) had a pH optimum of about 8, was inhibited with excess substrate (glutamine), and reaction products (glutamate and NH+ 4). A monocharged anion (Cl–) and a multicharged anion (phosphate) were shown to activate the glutaminase. Some features of the glutaminase from triticale were similar to those of animal glutaminase activated by phosphate and were different from features of the enzyme from Escherichia coli.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Evstigneeva, Z.G., Glutaminsintetaza: rol' v azotnom metabolizme rastenii, regulyatsiya i struktura. 41-oe Bakhovskoe chtenie (Glutamine Synthetase: Role in Nitrogen Metabolism in Plants, Regulation and Structure, 41st Bach Reading), Kretovich, V.L., Ed., Moscow: Nauka, 1988.
Prusiner, S., The Enzymes of Glutamine Metabolism, Prusiner, S. and Stadtman, E.R., Eds., New York: Academic, 1973, pp. 293-318.
Hartman, S.C., The Enzymes of Glutamine Metabolism, Prusiner, S. and Stadtman, E.R., Eds., New York: Academic, 1973, pp. 319-330.
Sayre, F. and Roberts, E., J. Biol. Chem., 1958, vol. 233, no. 5, pp. 1128-1134.
Ramadan, M., Asmar, F., and Greenberg, D.M., Arch. Biochem. Biophys., 1964, vol. 108, no. 1, pp. 143-149.
Evseev, L.P., Nikolaev, A.Ya., Eremenko, V.V., et al., Biokhimiya (Moscow), 1967, vol. 32, no. 4, pp. 837-875.
Streeter, J.G., Plant Physiol., 1977, vol. 60, no. 2, pp. 235-239.
Kretovich, V.L., Sidel'nikova, L.I., Kazakova, O.V., et al., Prikl. Biokhim. Mikrobiol., 1981, vol. 17, no. 6, pp. 821-825.
Kretovich, V.L., Sidel'nikova, L.I., Ivanushkin, A.G., and Karyakina, T.I., Prikl. Biokhim. Mikrobiol., 1984, vol. 20, no. 4, pp. 445-447.
Roberts, E., The Enzymes, Boyer, P.D., Ed., New York: Academic, 1960, vol. 4, pp. 285-295.
Katunuma, N., Katsunuma, N.,, and Tomino Towatary I., The Enzymes of Glutamine Metabolism, Prusiner, S. and Stadtman, E.R., Eds., New York: Academic, 1973, pp. 227-258.
Klingman, J.D. and Handler, P., J. Biol. Chem., 1958, vol. 232, no. 1, pp. 369-380.
Abdumalikov, A.Kh. and Nikolaev, A.Ya., Biokhimiya (Moscow), 1967, vol. 32, no. 4, pp. 859-866.
Lyubimov, V.I., L'vov, N.P., and Kirshteine, B.E., Prikl. Biokhim. Mikrobiol., 1968, vol. 4, no. 1, pp. 120-123.
Pryanishnikov, D.N., Azot v zhizni rastenii i zemledelii SSSR (Nitrogen in Plants Metabolism and Agriculture), Moscow: USSR Academy of Sciences, 1945.
Kretovich, V.L., Evstigneeva, Z.G., and Makarenko, M.N., Biokhimiya zerna (Grain Biochemistry), Kretovich, V.L., Ed., Moscow: USSR Academy of Sciences, 1954, vol. 2, pp. 161-178.
Kretovich, W.L., Adv. Enzymol., 1958, vol. 20, pp. 319-340.
Kretovich, V.L., Evstigneeva, Z.G., and Makarenko, M.N., Biokhimiya zerna (Grain Biochemistry), Kretovich, V.L., Ed., Moscow: USSR Academy of Sciences, 1954, vol. 2, pp. 45-111.
Glover, C.T. and Chibnall, A.C., Biochem. J., 1927, vol. 21, no. 2, pp. 857-860.
Solov'eva, N.A., Sidel'nikova, L.I., Shaposhnikov, G.L., Evstigneeva, Z.G., and Shatilov, V.R., Prikl. Biokhim. Mikrobiol., 1998, vol. 34, no. 2, pp. 189-192.
Chumikina, L.V., Solov'eva, N.A., Arabova, L.I., Sidel'nikova, L.I., Shaposhnikov, G.L., Malikov, S.F., Evstigneeva, Z.G., and Shatilov, V.R., Prikl. Biokhim. Mikrobiol., 1999, vol. 35, no. 6, pp. 695-703.
Kretovich, V.L. and Evstigneeva, Z.G., Dokl. Akad. Nauk SSSR, 1953, vol. 93, no. 1, pp. 6-8.
Dixon, M. and Webb, E., Enzymes, London, 1964. Translated under the title Fermenty, Dixon, M., Eds., Moscow: Mir, 1966, pp. 72-77.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sidel'nikova, L.I., Evstigneeva, Z.G., Solov'eva, N.A. et al. Characterization of Glutaminase from Triticale. Applied Biochemistry and Microbiology 37, 569–573 (2001). https://doi.org/10.1023/A:1012394832053
Issue Date:
DOI: https://doi.org/10.1023/A:1012394832053