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Effect of Chronic Manganese Treatment on Adenosine Tissue Levels and Adenosine A2a Receptor Binding in Diverse Regions of Mouse Brain

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Abstract

In the present study the effects of chronic manganese (Mn) treatment on adenosine A2a receptor binding in mouse brain have been assessed. Male albino mice were divided in two groups: In the Mn-treated group, the animals were injected intraperitoneally (i.p.) with MnCl2 (5 mg/kg/day) five days per week during 9 weeks; in the control group, they were injected likewise with a saline solution. A significant decrease of the Kd without alteration of Bmax in the cerebellum and, an increase of the Kd and Bmax in hippocampus of mice treated with Mn were found. Also, an increase of Kd in frontal cortex was observed. The binding parameters in caudate nucleus, olfactory bulb and hypothalamus were not altered by Mn. A significant decrease in the adenosine concentration in caudate nucleus, olfactory bulb and hypothalamus, without significant changes in hippocampus, frontal cortex and cerebellum was also detected. These findings suggest that chronic administration of Mn could affect adenosine receptor function and turnover, depending on the brain region analyzed.

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REFERENCES

  1. Huang, C. C., Chu, N. S., Lu, C. S., Wang, J. D., Tsai, J. L., Tzeng, J. L., Walters, E. C., and Calne, D. B. 1989. Chronic manganese intoxication. Arch. Neurol. 46:1104–1106.

    Google Scholar 

  2. Bonilla, E. 1987. El manganeso y su importancia biomédica. Ediciones Astro Data Maracaibo, Venezuela. pp. 2–79.

    Google Scholar 

  3. Doherty, J. D., Lourte, C. J., and Trams, E. G. 1983. Mn2+ stimulated ATPase in rat brain. Neurochem. Res. 8:493–500.

    Google Scholar 

  4. Cooper, D. M. F. and Caldwell, K. K. 1990. Signal transduction mechanisms for adenosine. In: Adenosine and adenosine receptors. Williams M. (Eds), The Humana Press, Clifton, New Jersey, pp. 105–141.

    Google Scholar 

  5. Bonilla, E. 1981. Manganismo crónico experimental: Aspectos neuroquímicos. Invest. Clin. 22:159–173.

    Google Scholar 

  6. Mena, I., Marín, O., Fuenzalida, S., and Cotzias, G. C. 1967. Chronic manganese poisoning. Clinical picture and manganese turnover. Neurology 17:128–136.

    Google Scholar 

  7. Bhargava, H. N. 1987. Effect of repeated administration of manganese on striatal cholinergic and dopaminergic receptors in the rat. Toxicol. Lett. 37:135–141.

    Google Scholar 

  8. Chandra, S. V. and Shukla, G. 1981. Effect of manganese on synthesis of brain catecholamines in growing rats. Acta Pharmacol. Toxicol. 48:349–354.

    Google Scholar 

  9. Bonilla, E. and Diez-Ewald, M. 1974. Effect of L-dopa on brain concentration of dopamine and homovanillic acid in rats after chronic manganese chloride administration. J. Neurochem. 22:297–299.

    Google Scholar 

  10. Barbeau, A. 1984. Manganese and extrapyramidal disorders. Neurotoxicol. 5:13–36.

    Google Scholar 

  11. Cano, G., Bonilla, E., Castro, F., Alburges, M., and Wamsley, J. 1992. Intoxicación crónica con manganeso. Cuantificación autorradiográfica de los receptores colinérgicos muscarínicos en el cerebro de ratón. Invest. Clin. 33:69–80.

    Google Scholar 

  12. Villalobos, V., Bonilla, E., Estévez, J., Dávila, J. O., and Castro, F. 1994. Manganese toxicity: Muscarinic receptor binding in the mouse brain. J. Toxicol. Environ. Health 42:185–191.

    Google Scholar 

  13. Díaz, H. L. and Gómez, G. A. 1993. Neurobiología de la intoxicación por manganeso. Rev. Chil. Neuro-Psiquiat. 31:351–358.

    Google Scholar 

  14. Coassin, M., Ursini, F., and Bindoli, A. 1992. Antioxidant effect of manganese Arch. Biochem. Biophys. 299:330–333.

    Google Scholar 

  15. Pugh, S. Y., Guiseppi, J. L., and Fridovich, I. 1984. Induction of superoxide dismutase in Escherichia coli by manganese and iron. J. Bacteriol. 160:137–142.

    Google Scholar 

  16. Shiffmann, S. N., Jacobs, O. P., and Vanderhaeghen, J. J. 1991. The striatal restricted adenosine A2 receptor (RDC) is expressed by enkephalin but not by substance P neurons. An in situ hibridization histochemistry study. J. Neurochem. 57:1062–1067.

    Google Scholar 

  17. Wojcik W. J. and Neff, N. H. 1983. Differential location of adenosine A1 and A2 receptors in striatum. Neurosci. Lett. 41:55–60.

    Google Scholar 

  18. Jarvis, M. F. and Williams, M. 1990. Adenosine in Central Nervous System. In: Adenosine and adenosine receptors. Williams M. (Eds), The Humana Press, Clifton, New Jersey, pp. 423–474.

    Google Scholar 

  19. Jarvis., M. F. and Williams, M. 1989. Direct autoradiographic localization of adenosine A2 receptors in the rat brain using the selective A2-selective agonist, [3H]CGS 21680. Eur. J. Pharmacol. 168:243–246.

    Google Scholar 

  20. Jarvis, M. F., Schulz, R., Hutchison, A. J., Do, U. H., Sills, M. A., and Williams, M. 1989. [3H]CGS 21680, a selective A2 adenosine receptor agonist, directly labels A2 receptors in rat brain. J. Pharmacol. Exp. Ther. 251:888–893.

    Google Scholar 

  21. Johansson, B., Parkinson, F. E., and Fredholm, B. 1972. Effects of mono-and divalent ions on the binding of the adenosine analogue CGS 21680 to adenosine A2 receptors in rat striatum. Biochem. Pharmacol. 44:2365–2370.

    Google Scholar 

  22. Latini, S., Pazzagli, M., Pepeu, G., and Pedata, F. 1996. A2 adenosine receptors. Their presence and neuromodulatory role in the central nervous system. Gen. Pharmacol. 27:925–933.

    Google Scholar 

  23. Fink, J. K., Weaver, D. R., Rivkess, S. A., Peterfrenund, R. A., Pollack, A., Adler, E. M., and Reppert, S. M. 1992. Molecular cloning of the rat A2 adenosine receptor: Selective coexpression with D2 dopamine receptors in rat striatum. Mol. Res. 14:186–195.

    Google Scholar 

  24. Albin, R. L. Young, A. B., and Penney, B. 1989. The functional anatomy of basal ganglia disorders. Trends Neurosci. 12:366-375.

    Google Scholar 

  25. Glowisnski, J. and Iversen, L. 1966. Regional studies of catecholamines in the rat brain. I. The disposition of 3H-norepinephrine, 3H-dopamine and 3H-DOPA in various regions of the brain. J. Neurochem. 13:655–669.

    Google Scholar 

  26. Bonilla, E., Arrieta, A., Castro, F., Dávila, J. O., and Quiroz, I. 1994. Manganese toxicity: Free aminoacids in striatum and olfactory bulb of the mouse. Invest. Clin. 35:175–181.

    Google Scholar 

  27. Lowry, O. H., Rosebrouh, N. J., Farr, A. L., and Randall, R. L. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  28. Bonilla, E. 1978. Flameless atomic absorption spectrophotometric determination of manganese in rat brain and other tissues. Clin. Chem. 24:471–474.

    Google Scholar 

  29. Munson, P. J. and Rodbar, D. 1980. Ligand. A versatile computarized approach for characterization of ligand bindingsystems. Anal. Biochem. 107:220–239.

    Google Scholar 

  30. Betto, P., Popoli, P., Ricciarello, G., Coporali, M. G., and Antonini, R. 1994. Simultaneous high-performance liquid chromatographic determination of adenosine and dopamine in rat striatal tissue with combined ultraviolet absorbance and electrochemical detection. J. Cromatogr. B. 662:21–25.

    Google Scholar 

  31. Turbochrom 4 Tutorial 1995. S270-0604-C PE Nelson Division. Perkin Elmer, USA.

  32. Barbeau, A. 1984. Manganese and extrapyramidal disorders. Neurotoxicol. 5:13–36.

    Google Scholar 

  33. Cardozo, J. and Bonilla, E. 1985. The neuropathology of experimental chronic manganese poisoning in rats. A preliminary report. Invest. Clin. 26:117–124.

    Google Scholar 

  34. Donaldson, J. 1987. The physiopathologic significance of manganese in brain. Its relation to schizophrenia and neurodegenerative disorders. Neurotoxicol. 8:451–462.

    Google Scholar 

  35. Gerfen, C. R., Engber, T. M., Mahan, L. C., Susel, Z., Chase, T. N., Monsma, F. J., and Sibley D. R. 1990. D1 and D2 dopamine receptor-regulated gene expression of striatonigral and striatopallidal neurons. Science 250:1429–1432.

    Google Scholar 

  36. Le Moine, C., Normand, E., Guitteny, A. F., Fouque, B., Teoule, R., and Bloch, B. 1990. Dopamine receptor gene expression by enkephalin neurons in rat forebrain. Proc. Natl. Acad. Sci. USA 87:230–234.

    Google Scholar 

  37. Gerfen, C. R. 1992. The neostriatal mosaic: Multiple levels of compartmental organization. Trends Neurosci. 15:133–139.

    Google Scholar 

  38. Pedata, F., Pazzagli, M., Tilii, S., and Pepeu, G. 1990. Regional differences in the electrically stimulated release of endogenous and radioactive adenosine and purine derivatives from rat brain slices. Naunyn. Schmiedeberg's Arch. Pharmacol. 342:447–453.

    Google Scholar 

  39. Ferré, S., Herrera-Marschitz, M., Grabowska-Ande, M., Ungerstedt, U., Casas, M., and Anden, N. E. 1991. Postsynaptic dopamine/adenosine interaction: Adenosine analogues inhibit a D2 mediated behaviour in short-term reserpined mice. Eur. J. Pharmacol. 192:30–35.

    Google Scholar 

  40. Ferré, S., Herrera-Marschitzz, M., Grabowska-Ande, M., Ungerstedt, U., Casas, M., and Anden, N. E. 1991. Postsynaptic dopamine/adenosine interaction: II Postsynaptic dopamine agonism and adenosine antagonism of methylxantines in short-term reserpined mice. Eur. J. Pharmacol. 192:36–42.

    Google Scholar 

  41. Richardson, P. J., Kase, H., and Jenner, P. G. 1980. Adenosine A2a receptor antagonists as new agents for the treatment of Parkinson's disease. Trends Pharmacol. Sci. 18:338–344.

    Google Scholar 

  42. Bonilla, E. 1980. L-Tyrosine hydroxylase activity in the rat brain after chronic oral administration of manganese chloride. Neurobehav. Toxicol. 2:37–41.

    Google Scholar 

  43. Cotzias, G. C., Papavasiliou, P. S., Mena, I., Tang, L. C., and Miller, S. T. 1974. Manganese and catecholamines. Adv. Neurol. 5:235–243.

    Google Scholar 

  44. Ferré, S., Rubio, A., and Fuxe, K. 1991. Stimulation of adenosine A2 receptors induces catalepsy. Neurosci. Lett. 130:162–164.

    Google Scholar 

  45. Johansson, B., Georgiev, V., Parkinson, F. E., and Fredholm, B. B. 1993. The binding of the adenosine A2 receptor selective agonist [3H]CGS 21680 to rat cortex differs from its binding to rat striatum. Eur. J. Pharmacol. 247:103–110.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, Venezuela

    Virginia Villalobos, Jacqueline Suárez & Jesús Estévez

  2. Instituto de Investigaciones Clínicas “Dr. Américo Negrette”, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela

    Ernesto Bonilla

  3. Instituto de Investigaciones, Facultad de Odontología, Universidad del Zulia, Maracaibo, Venezuela

    Ernesto Novo

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  1. Virginia Villalobos
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  4. Ernesto Novo
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  5. Ernesto Bonilla
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Villalobos, V., Suárez, J., Estévez, J. et al. Effect of Chronic Manganese Treatment on Adenosine Tissue Levels and Adenosine A2a Receptor Binding in Diverse Regions of Mouse Brain. Neurochem Res 26, 1157–1161 (2001). https://doi.org/10.1023/A:1012379024571

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