Skip to main content
SpringerLink
Log in

Site-Specific Nickase from Bacillus Species Strain D6

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

Three site-specific endonucleases were found in thermophilic strain Bacillus species D6. One of them, BspD6II, is an isoschizomer of Eco57I. The second, BspD6III, is present in the strain in very small amount; therefore, it has not been characterized. This paper is devoted to the third, BspD6I, which recognizes pentanucleotide site 5'-GAGTC-3' and cleaves only one DNA strand at a distance of 4 nucleotides from the site in the 3'-direction in the chain with the GAGTC sequence, i.e., it behaves as a site-specific nickase. Nickase N.BspD6I cleaves one DNA strand only in double-stranded DNA and does not cleave single-stranded DNA. Site-specific methylase SscL1I (an isohypectomer of M·HinfI) that methylates adenine in the sequence 5'-GANTC-3' prevents DNA hydrolysis by nickase BspD6I.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Abdurashitov, M. A., Belichenko, O. A., Shevchenko, A. V., and Degtyarev, S. H. (1996) Mol.Biol.(Moscow), 30, 1261–1267.

    Google Scholar 

  2. New England Biolabs Catalog (2000/ 01) New England Biolabs Inc., Beverly.

  3. Vasiljeva, L. Yu., Zheleznaya, L. A., and Matvienko, N. I. (2000) Biochemistry (Moscow), 65, 565–570.

    Google Scholar 

  4. Brown, N., and Smith, M. (1980) Meth.Enzymol., 65, 391–404.

    PubMed  Google Scholar 

  5. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc.Natl.Acad.Sci.USA, 74, 5463–5467.

    PubMed  Google Scholar 

  6. Promega fmol DNA Sequencing System Technical Manual (1992) Promega Corporation, Madison.

  7. Maniatis, T., Fritsch, E., and Sambrook, J. (1984) Molecular Cloning [Russian translation] Mir, Moscow.

    Google Scholar 

  8. Shapovalova, N. I., Zheleznaya, L. A., Matvienko, N. N., and Matvienko, N. I. (1994) Biochemistry (Moscow), 59, 485–493 (Russ.).

    Google Scholar 

  9. Smith, H. O., and Nathans, D. A. (1973) J.Mol.Biol., 81, 419–432.

    PubMed  Google Scholar 

  10. Vasiljeva, L. Yu., Zheleznaya, L. A., and Matvienko, N. I. (1998) Biochemistry (Moscow), 63, 212–218.

    Google Scholar 

  11. Dunn, J. J., and Studier, F. W. (1983) J.Mol.Biol., 166, 477–535.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Russian Academy of Sciences, Institute of Theoretical and Experimental Biophysics, Pushchino, Moscow Region, 142290, Russia

    L. A. Zheleznaya, T. A. Perevyazova, D. V. Alzhanova & N. I. Matvienko

Authors
  1. L. A. Zheleznaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. A. Perevyazova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. V. Alzhanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. I. Matvienko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zheleznaya, L.A., Perevyazova, T.A., Alzhanova, D.V. et al. Site-Specific Nickase from Bacillus Species Strain D6. Biochemistry (Moscow) 66, 989–993 (2001). https://doi.org/10.1023/A:1012369525809

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1012369525809

Search

Navigation