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Metabotropic Glutamate Receptors Stimulate Phospholipase D via Different Pathways in the Adult and Neonate Rat Hippocampus

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Abstract

In order to characterize the ontogenetic profile of metabotropic glutamate (mGlu) receptors coupled to phospholipase D (PLD) we examined the effects of selected mGlu agents on PLD activity in immature and adult rat hippocampus. The group I mGlu receptor agonist 3,5-dihydroxyphenylglycine stimulated PLD in immature tissue, but reduced the PLD response evoked by the nonselective mGlu receptor agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylate [(1S,3R)-ACPD] in adult hippocampus. (2R,1′S,2′R,3′S)-2-(2′-Carboxy-3′-phenylcyclopropyl)glycine (PCCG-13), a recently characterized selective antagonist of PLD-coupled mGlu receptors, displayed a much greater activity in reducing the PLD response to (1S,3R)-ACPD in adult than in neonate hippocampus. Our results lend support to the hypothesis that glutamatergic activation of PLD in the rat hippocampus is developmentally regulated.

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Authors and Affiliations

  1. Dipartimento di Farmacologia Preclinica e Clinica, Universitè di Firenze, Viale Pieraccini 6, 50139, Firenze, Italy

    Sabina Attucci, Serenella Albani-Torregrossa, Flavio Moroni & Domenico E. Pellegrini-Giampietro

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  1. Sabina Attucci
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  2. Serenella Albani-Torregrossa
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  3. Flavio Moroni
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  4. Domenico E. Pellegrini-Giampietro
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Attucci, S., Albani-Torregrossa, S., Moroni, F. et al. Metabotropic Glutamate Receptors Stimulate Phospholipase D via Different Pathways in the Adult and Neonate Rat Hippocampus. Neurochem Res 26, 1151–1155 (2001). https://doi.org/10.1023/A:1012327007733

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