Abstract
Genetic screening of 1200-palm wine yeasts lead to the selection of fourteen isolates with various genetic and physiological properties. Nine of the isolates were identified as Saccharamyces species, three as Candida species, one as Schizosaccharomyces species and one as Kluyveromyces species. Five of the isolates were wild type parents, two were respiratory deficient mutants (rho) and nine were auxotrophic mutants. Four isolates were heterozygous diploid (αa) and two were homozygous diploid (aaα α) for the mating a mating types were further identified on mating with type loci. Four Mat α and four Mat a types were further identified on mating with standard haploid yeast strains. Forty-five percent sporulated on starvation medium producing tetrads. Fifty-two percent of the four-spored asci contained four viable spores. Maximum specific growth rate [μmax] of the fourteen isolates range from0.13–0.26, five isolates were able to utilize exogenous nitrate for growth. Percentage alcohol production range between 5.8–8.8% for palm wine yeast, 8.5% for bakers’ yeast and 10.4% for brewers yeast. The palm wine yeast were more tolerant to exogenous alcohol but had a low alcohol productivity. Hybridization enhanced alcohol productivity and tolerance in the palm wine yeasts.
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References
Bassir O. Observation on the fermentation of palm wine. J Biol Appl Chem 1962; 6: 21-22.
Faparusi SL, Origin of initial microflora of palm wine of oil palm tree. (Elaeis guineensis). J Appl Bact 1973; 36: 559-565.
Owuama CL, Saunders JR. Physiological variants of Saccharomyces cerevisiae and Klockera apiculata from palm wine and cashew juice. J Appl Bact 1999; 68: 491-494.
Okafor N. Microbiology and biochemistry of oil palm wine. Adv Appl Microbiol 1978; 24: 237-254.
Beech RW, Davenport RR. Isolation purification and maintenance of yeast. In: Booth C. eds. Methods in microbiology.London Academic Press; 1971 Vol. 4; 153-182.
Ezeronye OU, Okerentugba PO. Production of genetic recombinants of yeast for the treatment of effluent from a Nigerian paper recycling plant. J Agric Biotech Env 1999; 1: 53-59 ISSN 1595-0468.
Ogur M, St John RA. A differential and diagnostic plating method for population studies of respiratory deficiency in yeast. J Bact 1956; 72: 500-504.
Howthorne DC, Mortimer R. Chromosome mapping in Saccharomyces centromere-linked genes. Genetics 1960; 45: 1085-1110.
Monod J. The growth of bacterial cultures. Ann Inst Pasteur 1950; 79: 390. Fzeronve & lo
AOAC. Official methods of analysis association of official analytic chemists. Washington DC 198412-25.
Barnett JA, Payne RW, Yarrow D. Yeast: Characteristics and identification. Cambridge University Press. 1983; 352-362.
De Kock SH, du Preez JC, Killian SG. Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures. J Ind Microbiol Biotech 2000; 24: 231-236.
Fowell RR. Factors controlling the sporulation of yeast: II The sporulation phase. J Appl Bact 1967; 30: 450-474.
Anuna MI, Sokari TG, Akpapunam MA. Effect of source of yeast (Saccharomyces sp) on alcohol content and quality of pineapple (Ananas comosus) wine, Discovery & Innov 1990; 2: 80-84.
Jimenez J, Benitez T. Genetic analysis of high ethanol tolerant wine yeast. Curr Genet 1987; 12: 421-428.
Miklos I, Sipiczki M. Breeding of distillers yeast by hybridization with a wine yeast. Appl Microbiol 1991; 35(5): 638-642.
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Ezeronye, O., Okerentugba, P. Genetic and physiological variants of yeast selected from palm wine. Mycopathologia 152, 85–89 (2001). https://doi.org/10.1023/A:1012323721012
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DOI: https://doi.org/10.1023/A:1012323721012